A5015726567- Viral Infections and Vectors
- Cytomegalovirus and herpesvirus research
- Herpesvirus Infections and Treatments
- Pneumonia and Respiratory Infections
- Viral-associated cancers and disorders
- Respiratory viral infections research
- Viral Infections and Outbreaks Research
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- Leptospirosis research and findings
- Animal Virus Infections Studies
- Microbial infections and disease research
- Congenital Anomalies and Fetal Surgery
- Vector-Borne Animal Diseases
- Mosquito-borne diseases and control
- Fire effects on ecosystems
- Systemic Lupus Erythematosus Research
- Viral Infections and Immunology Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Animal Disease Management and Epidemiology
- Enterobacteriaceae and Cronobacter Research
- Immunodeficiency and Autoimmune Disorders
- Supply Chain Resilience and Risk Management
- Complementary and Alternative Medicine Studies
- Antibiotic Use and Resistance
Rubber Research Institute of Sri Lanka
2023-2025
University of Kelaniya
2003-2025
Sri Lanka Institute of Nanotechnology
2023
Swedish University of Agricultural Sciences
2022
Swedish Society for Nature Conservation
2022
Umeå University
2022
Colombo North Teaching Hospital
2019-2020
University of Peradeniya
1994-1996
University of Cambridge
1992-1994
Cancer Research UK Manchester Institute
1994
Murine gammaherpesvirus 68 (MHV-68) is able to persist in spleen cells of infected mice. To determine the cell type harbouring persistent virus, from animals were separated into immunoglobulin (Ig)-positive (B cell-enriched), Ig-negative (T cell-enriched) and plastic-adherent (macrophage-enriched) fractions. These co-cultivated with permissive BHK-21 an infectious centre assay. The consistent recovery enrichment centres Ig-positive fraction clearly demonstrates that B are a major site virus...
The primary infection of BALB/c mice with murine herpesvirus 68 (MHV-68) was investigated. When the virus introduced intranasally, lung main tissue infected, being associated alveolar epithelium and mononuclear cells. A productive lasted for 10 days, after which viral DNA could be detected by in situ hybridization up to 30 days infection. At that time lymphoproliferative accumulations were also observed lung, formation germinal centres. Virus recovered from heart, kidney, adrenal gland...
Murine gammaherpesvirus is a natural pathogen of wild mice. The virus infects alveolar cells and spleen during the primary infection establishes latent/persistent in B lymphocytes. Little known about immunological response to gammaherpesviruses infection. To address this issue, we investigated pathogenesis murine herpesvirus 68 (MHV-68) mice deficient CD4 or CD8 T-cell populations. Infection lung were greatly exacerbated CD8-deficient mice, reflected by elevated titers an increase number...
<b>Background:</b> The association between herpes simplex virus type 2 (HSV-2) and human immunodeficiency (HIV) the development of HSV vaccines have increased interest in study epidemiology. <b>Objectives:</b> To estimate age sex specific seroprevalence HSV-1 HSV-2 infections selected populations Brazil, Estonia, India, Morocco, Sri Lanka. <b>Methods:</b> Serum samples were collected from various including children, antenatal clinic attenders, blood donors, hospital inpatients, HIV sentinel...
Human Cytomegalovirus (HCMV) spreads through direct contact with blood, and other body fluids such as urine, saliva breast milk. It is transmitted sexually can also be passed from a pregnant mother to fetus via the placenta. While often asymptomatic in healthy individuals, HCMV causes symptomatic infections immunocompromised people congenital fetuses. common global infection lead morbidity occasional mortality newborns. Latent may reactivate immunosuppressed individuals. This study aimed...
Two global (re-)emerging zoonoses, leptospirosis and hantavirus infections, are clinically indistinguishable. Thirty-one patients, hospitalized in Sri Lanka for acute severe leptospirosis, were after exclusion of other potentially involved pathogens, prospectively screened with IgM ELISA both pathogens. Of these, nine (29·0%) positive only, one (3·2%) seven (22·5%) pathogens concomitantly, whereas 13 (41·9%) remained negative both. Moreover, a retrospective study 23 former serologically...
Murine gammaherpesvirus 68 (MHV-68) is a natural pathogen of murid rodents and displays similar pathobiological characteristics to those the human Epstein-Barr virus (EBV). However, in contrast EBV, MHV-68 will replicate epithelial cells vitro. It has therefore been proposed that may be use as model for study gammaherpesviruses, EBV particular, both vitro vivo. The homolog herpes simplex glycoprotein B (gB), termed gp110, somewhat unusual compared with many other herpesviruses. We decided...
Mass vaccination programs should employ effective strategies to design a resilient vaccine supply chain for immunizing populations quickly and efficiently. The need more flexible responsive is highlighted during the pandemic, where authorities are required effectively execute distribution. Our study proposes scientifically driven approach identify suitable distribution, enhancing effectiveness of mass vaccination. We propose two-stage identifying best strategy that supports faster rollouts,...
Extended-spectrum β-lactamase producing Enterobacteriaceae (ESBL-PE) and carbapenem-resistant (CRE) are disseminated worldwide posing a serious public health concern. Although, the presence of ESBL-PE CRE in Sri Lanka has been reported, prevalence is unknown. This study aimed to provide up-to-date epidemiological data on multidrug-resistant characterize molecular determinants carbapenemase-producing (CPE) Lanka.A prospective cross-sectional was conducted at tertiary care hospital between...
Chronic Kidney disease of uncertain etiology (CKDu) has become a significant burden, affecting farming community Sri Lanka and the exact etiology, which could be multifactorial, is not hitherto established. This study aimed to determine association past hantavirus infection leptospirosis with occurrence CKDu. A cohort ( n = 179) known CKDu patients living in high-CKDu prevalent areas Anuradhapura district was compared group 49 healthy, sex-matched younger blood relatives (control-1) another...
Abstract Asthma is a significant public health concern, particularly in children. Exacerbation of asthma (EOA) life-threatening and respiratory infections (RIs) play crucial role. Though viruses role EOA, patients are empirically treated with antibiotics; particular, macrolides prescribed for unconfirmed M. pneumoniae infections. Although EOA have been reported many countries, no published data Sri Lanka. The present study aimed to identify common viruses, typical bacterial pathogens,...
Infections with Campylobacter spp. and Salmonella are the most frequently reported causes of human bacterial enteritis. Warm-blooded animals, including livestock, pets, wildlife, can be carriers bacteria may contaminate environment food products. The present study investigated occurrence in fecal pat samples from free-ranging toque macaques (Macaca sinica) tufted gray langurs (Semnopithecus priam) collected March–May 2015 Sri Lanka. In 58 macaques, jejuni was isolated 10 (17%), coli four...
Respiratory tract infections are a major cause of global morbidity and mortality. Pneumonia is the ninth leading mortality in Sri Lanka. Atypical pathogens about one-fifth community-acquired pneumonia, while Mycoplasma pneumoniae accounts for 50 %. This study aimed to determine seroprevalence M. respiratory Lanka attempting understand relationships between serology PCR.Paired sera from 418 adult patients (pneumonia, n=97; bronchitis, n=183; pharyngitis, n=138) 87 healthy controls were...
Mice inoculated intranasally with murine gammaherpesvirus-68 were used to evaluate the efficacy of acyclovir (ACV) in treatment acute and latent infections. Effectiveness was measured by infectious virus assay lung (site active replication) centre spleen cells latency). Intraperitoneal administration ACV at 6-h intervals starting soon after inoculation more effective reducing than 12-hourly injections commencing 3 days post-infection. Further, during infection resulted an approximately...
Rabies is a fatal viral disease that affects all mammals. It causes an estimated 59,000 human deaths worldwide annually. Dogs are the main reservoir and transmitter of rabies to in Sri Lanka. Prevention control measures include mass vaccination dogs post-exposure treatment. While these appear be homogeneous across country, there need identify community-level gaps due decentralization regional health veterinary services conducting such activities. A community-based questionnaire survey was...
Asthma is a significant public health concern, particularly in children with severe symptoms. Exacerbation of asthma (EOA) life-threatening and respiratory infections (RIs) play crucial role. Though viruses role EOA, patients are empirically treated antibiotics which contribute to the development antibiotic resistance. Although there widely reported association EOA viral or M. pneumoniae infections, no published data Sri Lanka. The present study aimed identify common viruses, typical...
Asthma is a significant public health concern, particularly in children with severe symptoms. Exacerbation of asthma (EOA) life-threatening and respiratory infections (RIs) play crucial role. Though viruses role EOA, patients are empirically treated antibiotics which contribute to the development antibiotic resistance. Although there widely reported association EOA viral or M. pneumoniae infections, no published data Sri Lanka. The present study aimed identify common viruses, typical...
Asthma is a significant public health concern, particularly in children with severe symptoms. Exacerbation of asthma (EOA) life-threatening and respiratory infections (RIs) play crucial role. Though viruses role EOA, patients are empirically treated antibiotics which contribute to the development antibiotic resistance. Although there widely reported association EOA viral or M. pneumoniae infections, no published data Sri Lanka. The present study aimed identify common viruses, typical...