A5065171794- Allergic Rhinitis and Sensitization
- Tracheal and airway disorders
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Airway Management and Intubation Techniques
- Foreign Body Medical Cases
- Respiratory and Cough-Related Research
- Sarcoidosis and Beryllium Toxicity Research
- IL-33, ST2, and ILC Pathways
- Single-cell and spatial transcriptomics
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Sinusitis and nasal conditions
- TGF-β signaling in diseases
- Meningioma and schwannoma management
- Monoclonal and Polyclonal Antibodies Research
- Cervical Cancer and HPV Research
- Tumors and Oncological Cases
- Vascular Malformations Diagnosis and Treatment
- Telemedicine and Telehealth Implementation
- Pregnancy and Medication Impact
- Diabetes Management and Research
- Nasal Surgery and Airway Studies
- Esophageal and GI Pathology
- Neurofibromatosis and Schwannoma Cases
Royal London Hospital
2020-2023
Queen Mary University of London
2023
Barts Health NHS Trust
2020-2021
Imperial College London
2009-2020
Asthma UK
2015-2020
Medical Research Council
2015-2020
Royal Ottawa Mental Health Centre
2020
Harefield Hospital
2020
Charing Cross Hospital
2016-2017
Wexham Park Hospital
2013
Integrated single-cell transcriptomic and BCR analyses reveal how antibody class switching influences human B cell fate function.
BackgroundChronic rhinosinusitis with nasal polyposis (CRSwNP) in Western countries is characterized by eosinophilia, IgE production, and TH2 cytokine expression. Type 2 innate lymphoid cells from polyps produce IL-5 IL-13 response to IL-25 IL-33, although the relevance of this axis local mucosal T-cell responses unknown.ObjectiveWe sought investigate role IL-25/IL-33 patients CRSwNP.MethodsPolyp tissue blood were obtained undergoing polypectomy. Control biopsy specimens healthy volunteers....
The germinal center (GC) response is critical for both effective adaptive immunity and establishing peripheral tolerance by limiting autoreactive B cells. Dysfunction in these processes can lead to defective immune responses infection or contribute autoimmune disease. To understand the gene regulatory principles underlying GC response, we generated a single-cell transcriptomic epigenomic atlas of human tonsil, widely studied representative lymphoid tissue. We characterize diverse cell...
Rationale: Increases in airway smooth muscle, extracellular matrix, and vascularity are prominent features of remodeling asthma, whereas the extent such patients with persistent allergic rhinitis (PAR) is unknown.Objectives: To test hypothesis that upper a feature PAR.Methods: Total nasal symptoms scores, biopsies, Th1 Th2 cytokines from lavage were assessed subjects severe PAR (n = 46) healthy control 19). Angiolymphangiogenesis was examined using immunohistochemistry staining against CD31...
Local tissue eosinophilia and Th2 cytokines are characteristic features of seasonal allergic rhinitis. Airway remodelling is a feature asthma whereas evidence for in rhinitis (AR) conflicting.By use novel human repetitive nasal allergen challenge (RAC) model, we evaluated the relationship between inflammation AR.Twelve patients with moderate-severe AR underwent 5 alternate day challenges diluent which after 4 weeks were followed by grass pollen extract. Nasal symptoms, Th1/Th2 secretion...
Pregnancy
Abstract In response to antigen challenge, B cells clonally expand, undergo selection and differentiate produce mature cell subsets high affinity antibodies. However, the interplay between dynamic states their antibody-based is challenging decipher in primary human tissue. We have applied an integrated analysis of bulk single-cell antibody repertoires paired with transcriptomics undergoing maturation. define unique gene expression known novel states, including a pre-germinal centre state...
Abstract The germinal center (GC) response is critical for both effective adaptive immunity and establishing peripheral tolerance by limiting auto-reactive B cells. Dysfunction in these processes can lead to defects immune pathogens or contribute autoimmune disease. To understand the gene regulatory principles underlying GC response, we generated a single-cell transcriptomic epigenomic atlas of human tonsil, widely studied representative lymphoid tissue. We characterize diverse cell subsets...
Discovery of insulin in 1921 changed the lives patients with type 1 diabetes (T1DM) forever. What had been a death sentence became manageable, albeit chronic, disease. Insulin did not cure disease, as it address actual disease process, but instead treated its sequelae, namely elevated blood sugars. Importantly, administration fails to ensure normoglycaemia. Even most sophisticated ‘near closed-loop’ methods, glucose homeostasis is restored normal. T1DM face complications, both short-term,...
We describe the importance of collaboration between multiple surgical specialties in managing a complex case sight-threatening severe proptosis young woman with type 2 neurofibromatosis (NF2) complicated by pre-existing contralateral blindness. Trans-nasal and lateral orbital approaches were aided stereotactic navigation to debulk large frontal/sphenoid wing meningioma, which had been exerting pressure onto right globe optic nerve. The patient made an excellent postoperative recovery along...
In response to antigen challenge, B cells clonally expand, undergo selection and differentiate produce mature cell subsets high affinity antibodies. However, the interplay between dynamic states their antibody-based is challenging decipher in primary human tissue. We have applied an integrated analysis of bulk single-cell antibody repertoires paired with transcriptomics undergoing maturation. define unique gene expression known novel states, including a pre-germinal centre state primed class...
A 14-month-old girl initially presented to the Accident and Emergency (A&E) department following a choking episode subsequent vomiting. The child left before being seen but re-presented morning with stridor, drooling, increased work of breathing. chest lateral neck soft tissue X-ray performed in A&E revealed an ingested button battery oesophagus. oesophagoscopy was 22 mm removed from oesophagus at level cricopharyngeus muscle, no immediate complications. Following extubation, patient...
Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | 1479-6848 (online)