A5069897350- Mitochondrial Function and Pathology
- RNA Research and Splicing
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- Adipose Tissue and Metabolism
- Cancer, Hypoxia, and Metabolism
- Pancreatic function and diabetes
- Genomics and Phylogenetic Studies
- Cancer-related gene regulation
- Autophagy in Disease and Therapy
- ATP Synthase and ATPases Research
- Ubiquitin and proteasome pathways
- Peptidase Inhibition and Analysis
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- RNA and protein synthesis mechanisms
- Protease and Inhibitor Mechanisms
- Circadian rhythm and melatonin
- Cell Adhesion Molecules Research
- Chemical Synthesis and Analysis
- Lipid metabolism and biosynthesis
- Invertebrate Immune Response Mechanisms
- Advanced Breast Cancer Therapies
- Plant Genetic and Mutation Studies
Zhejiang University-University of Edinburgh Institute
2018-2025
University of Edinburgh
2021-2025
Zhejiang University
2018-2025
University of Helsinki
2003-2023
Institute for Molecular Medicine Finland
2008-2023
Public Health Institute
2023
Second Affiliated Hospital of Zhejiang University
2021
University of Dundee
2013-2018
Helsinki University Hospital
2010
Aarhus University Hospital
2010
Overexpression screens are used to explore gene functions in Drosophila, but this strategy suffers from the lack of comprehensive and systematic fly strain collections efficient methods for generating such collections. Here, we present a that could be efficiently generate large numbers transgenic Drosophila strains, collection 1149 UAS-ORF lines were created with site-specific ΦC31 integrase method. For collection, set 655 genes cloned as two variants, either an open reading frame (ORF)...
Highlights•Mitochondrial functionality is highest in intermediate-sized cells a population•Mitochondrial membrane potential changes with cell size, not cycle•Evidence for an optimal whereby and fitness are maximized•Mitochondrial dynamics mevalonate pathway required the sizeSummaryEukaryotic attempt to maintain resulting size homeostasis. While cellular content scales isometrically allometric laws indicate that metabolism per mass unit should decline increasing size. Here we use elutriation...
BackgroundRegulation of cell size requires coordination growth and proliferation. Conditional loss cyclin-dependent kinase 1 in mice permits hepatocyte without division, allowing us to study vivo using transcriptomics metabolomics.ResultsLarger cells displayed increased expression cytoskeletal genes but unexpectedly repressed many involved mitochondrial functions. This effect appears be autonomous because cultured Drosophila induced increase a similar gene-expression pattern. Larger...
Article18 April 2018Open Access Source DataTransparent process Thermal proteome profiling of breast cancer cells reveals proteasomal activation by CDK4/6 inhibitor palbociclib Teemu P Miettinen Division Cell and Developmental Biology, University Dundee, UK MRC Protein Phosphorylation Ubiquitylation Unit, Laboratory for Molecular College London, Koch Institute Integrative Cancer Research, Massachusetts Technology, Cambridge, MA, USA Search more papers this author Julien Peltier Biosciences,...
N6-methyladenosine (m6A) is a critical regulator in the fate of RNA, but whether and how m6A executes its functions different tissues remains largely obscure. Here we report downregulation crucial reader, YTHDF2, leading to tissue-specific programmed cell deaths (PCDs) upon fluorene-9-bisphenol (BHPF) exposure. Currently, Bisphenol A (BPA) substitutes are widely used plastic manufacturing. Interrogating eight common BPA substitutes, detected BHPF 14% serum samples pregnant participants. In...
The analgesic and antipyretic compound acetaminophen (paracetamol) is one of the most used drugs worldwide. Acetaminophen overdose also common cause for acute liver toxicity. Here we show that many structurally related compounds bind quinone reductase 2 (NQO2) in vitro live cells, establishing NQO2 as a novel off-target. modulates levels derived reactive oxygen species, more specifically superoxide anions, cultured cells. In humans, highly expressed kidney, main sites We suggest mediated...
Ribosome profiling identifies ribosome positions on translated mRNAs. A prominent feature of published datasets is the near complete absence ribosomes in 3′ untranslated regions (3′UTR) although substantial density can be observed non-coding RNAs. Here we perform cultured Drosophila and human cells show that different features translation are revealed depending nuclease digestion conditions used. Most importantly, observe high abundance protected fragments 3′UTRs thousands genes without...
Balance between cell growth and proliferation determines size homeostasis, but little is known about how metabolic pathways are involved in the maintenance of this balance. Here, we perform a screen with library clinically used drug molecules for their effects on size. We find that statins, inhibitors mevalonate pathway, reduce increase cellular protein density various types, including primary human cells. Mevalonate pathway mediated through geranylgeranylation small GTPase RAB11, which...
The circadian clock regulates daily variations in physiologic processes. CLOCK acts as a regulator the apparatus controlling expression of other genes, including PER1. Clock genes have been implicated cancer-related functions; this work, we investigated possible target somatic mutations microsatellite unstable colorectal cancers. Combining microarray gene data and public sequence information, identified 1 790 putative novel instability (MSI) genes. A total 101 MSI carcinomas (CRC) were...
Transcription factors achieve specificity by establishing intricate interaction networks that will change depending on the cell context. Capturing these interactions in live condition is however a challenging issue requires sensitive and non-invasive methods. We present set of fly lines, called ‘multicolor BiFC library’, which covers most Drosophila transcription for performing Bimolecular Fluorescence Complementation (BiFC). The multicolor library can be used to probe two different binary...
The PML/RARα fusion protein is the oncogenic driver in acute promyelocytic leukemia (APL). Although most APL cases are cured by PML/RARα-targeting therapy, relapse and resistance can occur due to drug-resistant mutations. Here we report that thermal stress destabilizes protein, including clinically identified mutants. AML1/ETO TEL/AML1 oncofusions show similar heat shock susceptibility. Mechanistically, mild hyperthermia stimulates aggregation of complex with nuclear receptor corepressors...
Abstract Senescent cells typically have an enlarged cell size but the reason for this has not been fully elucidated. As abnormal may alter protein concentrations and cellular functionality, we used proteomic data from 59 unperturbed human lines to systematically characterize cell-size dependent changes in intracellular organelle content. Increase leads ubiquitous transcriptionally post-transcriptionally regulated reorganization dilution of proteome. Many known senescence proteins display...