Abstract Photothermal therapy (PTT) is of particular importance as a highly potent therapeutic modality in cancer therapy. However, critical challenge still remains the exploration effective strategy to maximize PTT efficiency due tumor thermoresistance and thus frequent recurrence. Here, rational fabrication micelles that can achieve mutual synergy molecularly targeted (MTT) for ablation reported. The generate both distinct photothermal effect from Cypate through enhanced conversion...

Psoriasis is a chronic inflammatory skin disease that involves the induction of T-helper 1 (Th1) and 17 (Th17) cell responses aberrant expression proinflammatory cytokines, including IL-1β. Copious evidence suggests abnormal activation Toll-like receptors (TLRs) contributes to initiation maintenance psoriasis. We have evaluated an antagonist TLR7, 8, 9 as therapeutic agent in IL-23-induced psoriasis model mice. Psoriasis-like lesions were induced C57BL/6 mice by intradermal injection IL-23...

Bacterial DNA and synthetic oligomers containing CpG dinucleotides activate the immune system through Toll-like receptor (TLR) 9. Here, we compare immunostimulatory activity of three immunomers with different nucleotide sequences a cytosine-phosphate-2'-deoxy-7-deazaguanosine dinucleotide (CpR), called immunomodulatory oligonucleotides (IMOs), in mouse, human, monkey systems. IMOs induced IL-12 IFN-gamma secretion more than control non-CpG IMO mice. All activated HEK293 cells expressing TLR9...

Oligonucleotides containing an immune-stimulatory motif and immune-regulatory act as antagonists of Toll-like receptor (TLR)7 TLR9. In the present study, we designed synthesized oligonucleotide-based TLR7, 8 9 a 7-deaza-dG or arabino-G modification in 2′-O-methylribonucleotides motif. We evaluated biological properties these novel synthetic oligoribonucleotides TLRs 7, murine human cell-based assays vivo mice non-human primates. HEK293, mouse assays, antagonist compounds inhibited signaling...

Cutaneous dermatophyte infections, dermatophytosis, are common worldwide, affecting various age groups and body parts. Despite advancements in antifungal therapies, the prevalence of dermatophytosis continues to increase, posing significant challenges for diagnosis treatment. This review provides an overview prevalence, clinical features, diagnostic methods Korea. A comprehensive domestic reports research on Korea was conducted using data from KoreaMed. study covers presentations,...

Bacterial DNA and synthetic oligonucleotides containing unmethylated CpG dinucleotides (CpG DNA) activate the vertebrate immune system promote Th1-like responses. Several DNAs are currently being tested in clinical trials as either alone or combination with vaccines, antibodies, allergens separately conjugates for a number of disease indications including cancers, allergies, asthma. In this paper, we show that conjugation an oligonucleotide through their 5'-ends (5'-5'-linked significantly...

Viral and synthetic single-stranded RNAs are the ligands for Toll-like receptor (TLR)7 TLR8. However, RNA is rapidly degraded by ubiquitous RNases, studies reported to date have used with lipid carriers. To overcome nuclease susceptibility of RNA, we synthesized several incorporating a range chemical modifications. The present study describes one pool compounds, referred as stabilized immune modulatory (SIMRA) in which two segments attached through their 3' ends. SIMRA compounds showed...

Oligodeoxynucleotides (ODNs) containing unmethylated CpG dinucleotides act as agonists of TLR9 and induce Th1-type immune responses. In the present study, we synthesized ODNs in which C or G was substituted with 2'-O-methylribonucleotides, 5-methyl-dC, 2'-O-methyl-5-methyl-C studied their stimulatory activity alone combination TLR agonists. mouse human primary cell-based assays, modified did not stimulate responses but inhibited agonist-induced activity. mice, cytokines induced by TLR7 TLR9....

Abstract BACKGROUND MDM2 oncogene is overexpressed in many human cancers including prostate cancer and levels are associated with poor prognosis. This study was undertaken to investigate the functions of growth value as a drug target for therapy by inhibiting expression. METHODS Antisense anti‐human‐MDM2 mixed‐backbone oligonucleotide its mismatch control were tested vitro vivo models (LNCaP, DU 145, PC‐3) anti‐tumor activity. Targeted gene products related proteins analyzed activity...

Hypoxia is widely accepted as a fundamental biological phenomenon, which strongly associated with tissue damage and cell viability under stress conditions. Insulin-like growth factor‑1 (IGF‑1) known to protect tissues from multiple types of damage, cells apoptosis. regulatory factor the IGF system, however role IGF-1 receptor (IGF‑1R) in hypoxia‑induced apoptosis remains unclear. The present study investigated potential mechanisms IGF‑1R‑associated hypoxic Mouse embryonic fibroblasts...

Bacterial and synthetic DNAs containing CpG dinucleotides in specific sequence contexts activate the vertebrate immune system through Toll-like receptor 9 (TLR9). In present study, we used a nucleoside with bicyclic heterobase [1-(2′-deoxy-β- d -ribofuranosyl)-2-oxo-7-deaza-8-methyl-purine; R] to replace C CpG, resulting an RpG dinucleotide. The dinucleotide was incorporated mouse- human-specific motifs oligodeoxynucleotides (oligos) 3′-3-linked oligos, referred as immunomers. Oligos motif...

Abstract Purpose: The MDM2 oncogene, amplified or overexpressed in many human cancers, has been suggested to be a novel target for cancer therapy. We have demonstrated second-generation antisense antihuman-MDM2 oligonucleotide antitumor activity when administered alone combination with chemotherapeutic agents. In the present study, we investigated effect of on radiation Experimental Design: vitro radiosensitization was determined cell lines cancers prostate (LNCaP and PC3), breast (MCF-7...

Oligodeoxyribonucleotides containing unmethylated CpG motifs act as TLR9 agonists. In this study, we evaluated oligonucleotides an motif in which two nucleotides adjacent to the dinucleotide were substituted with 2'-O-methylribonucleotides, resulting TLR7 and antagonists. mouse human cell cultures, antagonists did not stimulate immune activation but inhibited agonist-induced activity. mice, responses induced by agonists for up several days, inhibition was dose-dependent. Antagonists also...

Abstract Viral and synthetic single-stranded RNAs are the ligands for Toll-like receptors 7 8 (TLR7 TLR8). We have reported a novel class of oligoribonucleotides, referred to as stabilized immune-modulatory RNA compounds, which act agonists TLR7, TLR8, or both TLR7 TLR8 depending on sequence composition presence specific chemical modifications. In present study, we evaluated antitumor activity dual TLR7/8 agonist in tumor-bearing mice with peritoneal disseminated CT26.CL25 colon 3LL-C75 lung...

We evaluated the recent trend in incidence of premalignant and malignant skin lesions between 1991 2006. Among 571,057 newly registered dermatology out-patients from our 8 affiliated university hospitals, 2,598 were diagnosed with a (899, 0.16%) or (1,699, 0.30%). Of 899 cases, 71.2% actinic keratosis (AK), 24.6% Bowen's disease. 1,699 46.2% basal cell carcinoma, followed by squamous carcinoma (19.1%) melanoma (7.1%). This 16-yr survey was divided equally into two time periods to compare at...

Ultraviolet radiation causes skin damage due to increased production of reactive oxygen species (ROS) and inflammatory intermediates direct attack DNA cells. Astaxanthin is a reddish pigment that belongs group chemicals called carotenoids has protective effects as an antioxidant.To determine the beneficial astaxanthin on damaged human after exposure ultraviolet radiation.Normal epidermal keratinocytes (NHEKs) were pre-treated with for 24 hours exposed B (UVB) irradiation. After hours, Cell...

The MDM2 oncogene is amplified or overexpressed in many human cancers and levels are associated with poor prognosis. not only serves as a negative regulator of p53 but also has p53-independent activities. This study investigates the functions colon cancer growth potential value drug target for therapy, by inhibiting expression an antisense anti-human-MDM2 oligonucleotide.The selected mixed-backbone oligonucleotide was evaluated its vitro vivo antitumor activity models: LS174T cell line...

Background: Balneotherapy, although not a well-established dermatological treatment, is thought to have therapeutic properties for psoriasis and used as an alternative treatment modality throughout the world.Objective: To evaluate mechanism underlying immunologic effects of thermomineral water.Methods: A murine model imiquimod-induced psoriasis-like skin inflammation was evaluating balneotherapy with Hae-Un-Dae hot spring mineral water.The clinical improvements were evaluated by...

A 64-year-old female presented with facial hyperpigmentation. She had dyed her hair monthly pure henna powder for the past seven months. After patch tests, patient was diagnosed as post-inflammatory hyperpigmentastion due to allergic contact dermatitis that has rarely been reported. The underwent Q-switched Nd:YAG laser treatment and treated oral tranexamic acid 10 weeks. hyperpigmentation on forehead demonstrated substantial improvement.