A5069082513- Biofuel production and bioconversion
- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Nerve injury and regeneration
- Fungal and yeast genetics research
- Animal Genetics and Reproduction
- Neuroscience and Neuropharmacology Research
- Microbial Metabolic Engineering and Bioproduction
- Congenital heart defects research
- Plant nutrient uptake and metabolism
- Signaling Pathways in Disease
- Zebrafish Biomedical Research Applications
- MicroRNA in disease regulation
- Genomics and Phylogenetic Studies
- Aquaculture disease management and microbiota
- RNA Interference and Gene Delivery
- Growth Hormone and Insulin-like Growth Factors
- Muscle Physiology and Disorders
- Developmental Biology and Gene Regulation
- Tissue Engineering and Regenerative Medicine
- Receptor Mechanisms and Signaling
- Neuroscience of respiration and sleep
- Phagocytosis and Immune Regulation
- Photochromic and Fluorescence Chemistry
- Silk-based biomaterials and applications
National Institute of Advanced Industrial Science and Technology
2009-2023
Ministry of Economy, Trade and Industry
2011
University of Fukui
1997-2002
Osaka Medical and Pharmaceutical University
1997
Neuroblasts produced in the ventricular zone of neocortex migrate radially and form cortical plate, settling an inside-out order. It is also well known that tangential cell migration not negligible embryonic neocortex. To have a better understanding cortex, we disturbed by making cut neocortex, labeled migrating cells with 1,1'-dioctodecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) vivo vitro. We determined birth dates cells. Disturbance caused accumulation disappearance...
The pentose phosphate pathway (PPP) plays an important role in the synthesis of ribonucleotides and aromatic amino acids. During bioethanol production from cellulosic biomass composed mainly d-glucose d-xylose, PPP is also involved xylose metabolism by engineered Saccharomyces cerevisiae. Although activities thermostabilities four enzymes (transaldolase: TAL1, transketolase: TKL1, ribose-5-phosphate ketol-isomerase: RKI1 d-ribulose-5-phosphate 3-epimerase: RPE1) can affect efficiency ethanol...
Abstract The ganglionic eminence (GE) supplies neurons containing γ‐aminobutyric acid (GABA) to the pallium of telencephalon. We investigated molecular guidance mechanisms GE cell migration in neocortex and found neuropilin‐1 (Npn‐1) or neuropilin‐2 (Npn‐2) on cells. Ectopic Sema3A ‐3F expression by COS1 clusters placed embryo neocortical slices reduced but did not block it completely. However, was almost completely blocked expressing both ‐3F. direction could be reversed placing Sema3A‐...
Expression of d-xylose isomerase having high catalytic activity in Saccharomyces cerevisiae (S. cerevisiae) is a prerequisite for efficient and economical production bioethanol from cellulosic biomass. Although previous studies demonstrated functional expression several xylose isomerases (XI) S. cerevisiae, identification XIs higher needed. Here, we report new strategy to improve fermentation the strain IR-2 that involves an evolutionary engineering select top-performing eight previously...
The behavior of single molecules neurotrophins on growth cones was observed by the use fluorescent conjugate nerve factor (NGF), Cy3-NGF. After application 0.4 nm Cy3-NGF, chick dorsal root ganglion responded within 1 min adding stimulus expanding their lamellipodia. Only 40 which occupied <5% estimated total binding sites a cone, were required to initiate motile responses. high-affinity receptor, Cy3-NGF displayed lateral diffusion membrane with constant 0.3 microm2 s(-1). shifted...
microRNAs (miRNAs) in fish have not been as extensively studied those mammals. The species Takifugu rubripes is an intensively model organism whose genome has sequenced. T. approximately eight times smaller than the human genome, but a similar repertoire of protein-coding genes. Therefore, it useful for identifying non-coding genes, including miRNA To identify expression patterns different organs and give fundamental information to aid understanding populations this species, we extracted...
The onset of Hex expression and its role in early murine development was analyzed using situ hybridization. mRNA first detected the chorion ectoplacental cavity weakly at visceral endoderm future yolk sac embryonic age (E) 7.5. Expression tissues exclusively hepatic anlage thyroid primordium E 9.5. At 12.5 15.5, persisted fetal liver thyroid, also lung. These results suggest that has differentiation and/or organogenesis several tissues.
Aspergillus oryzae has been utilized for over 1000 years in Japan the production of various traditional foods, and a large number A. strains have isolated and/or selected effective fermentation food ingredients. Characteristics genetic alterations among used are particular interest studies oryzae. Here, we sequenced whole genome an industrial fungal isolate, RIB326, by using next-generation sequencing system compared data with those RIB40, wild-type strain 2005. The aim this study was to...
Little is known about the role of integrin-associated protein (IAP, or CD47) in neuronal development and its function central nervous system. We investigated responses IAP-overexpressing cortical neurons using a virus-gene transfer found that dendritic outgrowth was significantly enhanced IAP (form 4)-transfected neurons. Furthermore, synaptic proteins including synaptotagmin, syntaxin, synapsin I, SNAP25 (25-kDa synaptosomal associated protein) were up-regulated. In accordance with this...
In the telencephalon, corticospinal (CS), callosal, and hippocampal commissural neurons are major types of that have axons crossing midline brain. To understand mechanisms involved in structure to examine whether expression patterns L1 TAG-1 similar those spinal cord, we investigated these rats by using a double-labeling technique involving retrograde labeling situ hybridization. Expression messenger RNA was detected retrogradely labeled CS projection 1,1;-dioctadecyl-3,3,...
Heat shock protein promoters (hsp promoters) are powerful tools for investigating gene functions, as the expression of targeted genes can be controlled simply by heating. However, there have been no reports utilization an endogeneous medaka (Oryzias latipes) hsp promoter to induce exogenous in medaka. We identified and cloned a functional (olphsp70.1) verified its ability act inducible both vitro vivo. The efficiently induced cultured cells, developing embryos, also adult fishes. When used...
Feline McDonough Sarcoma (FMS)-like tyrosine kinase 4 (FLT4) is a marker for lymphatic vessels and some high endothelial venules in human adult tissues. We generated transgenic medaka fish which the blood are visible vivo by transferring promoter of flt4 driving expression enhanced green fluorescent protein (EGFP) using see-through line. To do this, we identified cloned construct was 4-kb region upstream translation initiation site. The signal EGFP could be observed with little background,...
We sequenced the genome of Saccharomyces cerevisiae IR-2, which is a diploid industrial strain with flocculation activity and ability to efficiently produce bioethanol. The approximately 11.4-Mb draft information provides useful insights into metabolic engineering for production bioethanol from biomass.
Abstract Background Pmp22 , a member of the junction protein family Claudin/EMP/PMP22, plays an important role in myelin formation. Increase pmp22 transcription causes peripheral neuropathy, Charcot-Marie-Tooth disease type1A (CMT1A). The pathophysiological phenotype CMT1A is aberrant axonal myelination which induces reduction nerve conduction velocity (NCV). Several model rodents have been established by overexpressing . Thus, it thought that expression must be tightly regulated for correct...
REGENERATION of injured axons at neuromuscular junctions has been assumed to be regulated by extracellular factors that promote neurite outgrowth. We report here the cloning a novel outgrowth factor, designated neurocrescin, from chick denervated skeletal muscle. A recombinant neurocrescin promoted cultured neurons spinal cord and telencephalon embryo. It was expressed predominantly in neural tissue muscle, secreted extracellularly after intramolecular cleavage. This truncated form detected...
Abstract Microtubule‐associated protein 2 (MAP2) occurs in developing mammalian neuronal tissue as both high‐ and low‐molecular‐weight forms with temporally regulated expression. We studied the MAP2 expression rat telencephalon monoclonal antibodies that recognized of variants or specifically high‐molecular‐weight variants. Differences staining patterns these reflected differences distribution MAP2s. The immunoreactive sites had a more widespread embryonic than those MAP2. Many bipolar cells...
The long-term effects of the 77-kDa muscle-derived protein (MDP77) on motor and sensory nerve regeneration were examined in vivo. Fourteen-millimeter bridge grafts right sciatic SD rats carried out with silicone tubes containing a solution type I collagen together 0, 5, 10, or 20 microg/ml recombinant human MDP77 (N = 10 each group). Recovery function was evaluated monthly by maximal toe-spread index (TSI) hot-plate test, respectively, for 6 months after operation. Electrophysiology (nerve...
We report the draft genome sequence of Monascus purpureus GB-01, an industrial strain used as a food colorant. De novo assembly long reads resulted in 121 chromosomal contigs and 1 mitochondrial contig, sequencing errors were corrected by paired-end short reads. This will provide useful information for azaphilone pigments mycotoxin citrinin biosynthesis.