<i>Background:</i> Previous reports related the presence of mouse mammary tumor virus (MMTV)-like gene sequences to human breast carcinoma. The aim this study was determine whether MMTV-like <i>env</i> are present in cancer samples Mexican women and lung cell lines. <i>Methods:</i> Using specific primers for MMTV, we tested 3 lines, 4 non-small lines 119 from women. <i>Results:</i> were amplified INER-51, but not MCF-7 line that has been used...

The global incidence of melanoma is increasing. Mortality from influenced primarily by metastasis in advanced stages the disease. Current treatments are largely ineffective; thus, novel gene delivery approaches that target tumor-specific markers may be useful for treatment melanoma. Systemic administration encapsulated RNA‑interference plasmids targeted against tumor cells a potential alternative therapy cancer. Formulations transferrin (Tf)-conjugated polyethylene glycol (PEG) liposomes...

The Wilms' tumor protein 1 (WT1) is essential for cell proliferation and highly expressed in various hematological solid malignancies including human malignant melanoma. We investigated whether WT1 expression growth the B16F10 murine melanoma line. Toward this end, we examined isoforms (17AA+/17AA-, KTS+/KTS-) was silenced by two RNA interference constructs, designated WT1-1 WT1-2. interference-mediated reduction of significantly inhibited viability. Loss also increased caspase-3...

Cuphea aequipetala (C. aequipetala) has been used in Mexican traditional medicine since prehispanic times to treat tumors. In this paper, we evaluated the antiproliferative and apoptotic effect of methanolic aqueous extracts C. on several cancer cell lines including B16F10 line murine melanoma carried a model assay. vitro assay analyzed cellular cycle indicators apoptosis, such as caspase-3 activity, DNA fragmentation, phosphatidylserine exposure (Annexin-V), induction membrane...

Background: Skin cancers are common, and there has recently been a dramatic increase in their incidence, particularly the occurrence of melanoma. Furthermore, relapse after curative surgical treatment melanoma remains significant clinical challenge accounts for most mortality this disease. Objective: The aim study was to determine whether IMMUNEPOTENT CRP affects B16F10 cells tumors growth vascular endothelial factor (VEGF) production vivo vitro.Methods: B16F10-inoculated mice were treated...

Acalypha monostachya (A. monostachya) is a plant that used in traditional medicine as cancer treatment; however, its effect has not been validated. In this study, the potential cytotoxic effects and morphological changes of A. were evaluated human tumor cell lines. The aqueous (AE), methanolic (ME), hexane (HE) extracts obtained, flavonoid-type phenolic compounds detected, which indicates an antineoplastic effect. We observed time-dependent concentration-selective toxicity cells....

Prostate apoptosis response 4 (PAR4), a tumour-suppressor protein, selectively induces of cancer cells without affecting normal cells. Its soluble form is induced by secretagogues (e.g., chloroquine), and it interacting with the receptor glucose-regulated protein 78, which overexpressed in In this study, curcumin was analyzed as an inducer PAR4 expression 4T1 murine breast cell. its ability to induce secretion Balb/c mice. addition, cisplatin sensitizing effect analyzed.The cell line treated...

Background and Objectives: Nutritional deficiencies are one of the main triggers for development gastrointestinal diseases, such as ulcerative colitis (UC). Therefore, objective present work consisted determining nutrients in bone broth (BB) evaluating their anti-inflammatory properties a murine model UC, induced by intrarectal administration 2, 4, 6-trinitrobenzene sulfonic acid (TNBS), acetic (AcOH). The BB was prepared from femur bovine cattle cooked distilled water 8 h at 100 ± 2 °C....

Free amino acids and acylcarnitines circulating in the blood can be used for diagnosis metabolic illness imbalances. To date, normal reference ranges of horse peripheral have not been established. In this study, concentrations 12 26 were determined by tandem mass spectrometry complete from 100 healthy horses (50 Quarter (QH) [23 males 27 females] 50 American Miniature (AMH) [15 35 females]) with no signs disease. The means standard deviations data statistically analyzed. Concentrations...

Human papillomavirus (HPV) is an epitheliotropic, double-stranded DNA virus, and its high-risk genotypes are associated with human cancer. HPV genome has been detected in lung carcinomas certain places around the world, including Mexico; however, prevalence of this unclear. In study, we examine frequency 16/18 cancer tissues from a Mexican population.39 specimens were analyzed by polymerase chain reaction (PCR) using GP5+/GP6+ primers then genotyped specific to 16/18. Additionally, situ...

OBJETIVO: Detección molecular del virus de la hepatitis E (VHE) en hígado cerdo para consumo humano Nuevo León, México. MATERIAL Y MÉTODOS Se analizaron 127 hígados (87 obtenidos rastros TIF, y 40 carnicerías) mediante RT-PCR semianidado amplificar un fragmento 212 pb gen ORF2 VHE. RESULTADOS: El 19.5% (17) los 22.5% (9) carnicerías fueron positivos. La secuenciación mostró 94-95% homología con el genotipo 3. CONCLUSIONES: Los resultados indican que VHE circula granjas porcinas estado, lo...

Previous studies have reported on the presence of Murine Mammary Tumor Virus (MMTV)-like gene sequences in human cancer tissue specimens. Here, we search for MMTV-like lung diseases including carcinomas specimens from a Mexican population. This study was based our previous reporting that INER51 cell line, pleural effusion patient, contains env sequences.The been detected three out 18 studied, by PCR using specific set primers. The identified sequences, which were assigned as INER6, HZ101,...

Our main focus was to evaluate the efficacy of silver and sodium dichloroacetate as dual-function agents be used in melanoma treatment. This strategy is designed increase activity these two compounds that affect DNA integrity mitochondria at different levels. Furthermore, we evaluated if cell death mechanism induced by our treatments immunogenic death. To antitumor efficacy, assessed tumor volume production necrosis factor-α, nuclear factor κ B (both ELISA), nitric oxide levels...

The Wilms' tumour gene (WT1) has previously been described as an oncogene in several neoplasms of humans, including melanoma, and its expression increases cancer cell proliferation. Recent reports associate the PPARβ/δ (peroxisome proliferator-activated receptor beta/delta) with downregulation WT1 human melanoma murine lines. aim this work was to analyse association samples healthy melanoma-affected skin horses by immunohistochemistry. protein detected skin, mainly epidermis, hair follicle,...

The increased incidence of cancer in recent years is associated with a high rate mortality. Numerous types have low percentage CD133(+) cells, which similar features to stem cells. CD133 molecule involved apoptosis and cell proliferation. aim this study was determine the biological effect suppression its role chemosensitization lines. RT-PCR immunocytochemical analyses indicated that expressed lines B16F10, MCF7 INER51. Downregulation by transfection an antisense sequence (As-CD133) resulted...

Objective. To identify, describe and determine the frequency of species genus Eimeria from goat feces state Nuevo Leon, Mexico. Materials methods. From 403 samples excreta 13 municipalities Subsequently, after sporulation parasite dispersal forms (Oocysts), identification was made according to their morphology morphometry by means histograms linear regression analysis. Results. A total 60.29% animals sampled were positive for presence oocysts spp. The in which this most frequently detected...

Veterinary oncology is very important nowadays to get a better understanding of human carcinogenesis. Estrogen receptor, progesterone receptor and Human Epidermal Growth Factor 2 are frequently evaluated by immunohistochemistry (HIC) in breast tumor. WT1 an oncogene, its overexpression has been detected leukemia diverse solid tumors like cancer, lung cancer mesothelioma humans. expression was 15 canine (CBT) diagnosed histopathological analysis find relationship with neoplasia malignancy....

Blood samples were obtained from 16 high-risk heifers; eight newly arrived a 40 h road trip (0 days post-arrival (DPA)), whereas the other heifers had been in feedlot at 25 DPA. Both groups transported southeast tropical region of Mexico to northeast and sampled on same day. The complete blood count, chemistry, cytokine gene expression analyzed. Gene was analyzed using specific primers amplify quantify cDNA reverse transcribed mRNA transcripts for tumor necrosis factor (TNF)-α, interferon...

Malassezia species are yeasts that can be present on healthy human skin but also associated with external otitis and other diseases. The aim of this work was the identification -lipophilic- recovered from infants media. Microbiological culture, polymerase chain reaction (PCR) sequencing were performed for detection in samples obtained two cases We identified strains lipid-dependent species: M. furfur restricta. This is first report regarding presence media Monterrey, México. Key...