A5026463889- Mosquito-borne diseases and control
- Insect Pest Control Strategies
- Insect Resistance and Genetics
- Malaria Research and Control
- Insect-Plant Interactions and Control
- Insect symbiosis and bacterial influences
- Viral Infections and Vectors
- Dengue and Mosquito Control Research
- Insect and Pesticide Research
- Entomopathogenic Microorganisms in Pest Control
- Pesticide Exposure and Toxicity
- Cholinesterase and Neurodegenerative Diseases
- Vector-borne infectious diseases
- Trypanosoma species research and implications
- Education and Teacher Training
- Insect and Arachnid Ecology and Behavior
- Plant and animal studies
- Peptidase Inhibition and Analysis
- Animal testing and alternatives
- Immunotoxicology and immune responses
- Phytoplasmas and Hemiptera pathogens
- Latin American and Latino Studies
- Toxic Organic Pollutants Impact
- Neuroendocrine Tumor Research Advances
- Spanish Literature and Culture Studies
Universidad Autónoma de Nuevo León
2014-2023
Laboratoire Physiologie Cellulaire & Végétale
2017
Universidad Nacional Autónoma de México
1989-2016
Colorado State University
2014
Centre Paul Broca
1992
Inserm
1992
Background Aedes aegypti, the ‘yellow fever mosquito’, is primary vector to humans of dengue and yellow flaviviruses (DENV, YFV), a known chikungunya alphavirus (CV). Because vaccines are not yet available for DENV or CV inadequately distributed in developing countries (YFV), management Ae. aegypti remains option prevent control outbreaks diseases caused by these arboviruses. Permethrin one most widely used active ingredients insecticides suppression adult aegypti. In 2007, we documented...
Abstract Aedes aegypti is the primary urban mosquito vector of viruses causing dengue, Zika and chikungunya fevers –for which vaccines effective pharmaceuticals are still lacking. Current strategies to suppress arbovirus outbreaks include removal larval-breeding sites insecticide treatment larval adult populations. Insecticidal control Ae. challenging, due a recent rapid global increase in knockdown-resistance (kdr) pyrethroid insecticides. Widespread, heavy use space-sprays has created an...
Abstract BACKGROUND The V1016I and F1534C mutations in the voltage‐gated sodium channel gene have been associated with resistance to pyrethroids DDT Aedes aegypti mosquitoes. A study was carried out determine frequency of I1016 C1534 by real‐time PCR five natural populations Ae. Venezuela during 2008, 2010 2012, as well a strain selected 0.14 µg deltamethrin for 15 generations. RESULTS In populations, frequencies varied between 0.01 0.37, 0.35 1.0. Pampanito strain, increased from 0.02 F 1...
Potential insecticide-resistance mechanisms were studied with the use of biochemical assays in Aedes aegypti (L.) collected from 5 municipalities representing north part Quintana Roo: Benito Juarez, Cozumel, Isla Mujeres, Lazaro Cardenas, and Solidaridad. The activities α β esterases, mixed-function oxidases (MFO), glutathione-S-transferase (GST), acethylcholinesterase (AChE), insensitive (iAChE) assayed microplates. Three replicates performed for each enzyme 60 males females analyzed...
Seven F1 strains of Aedes aegypti (L.) were evaluated by bottle bioassay for resistance to the pyrethroids d-phenothrin, permethrin, deltamethrin, λ-cyalothrin, bifenthrin, cypermethrin, α-cypermethrin, and z-cypermethrin. The New Orleans strain was used as a susceptible control. Mortality rates after 1 h exposure 24 recovery period determined. ratio between 50% knockdown values (RRKC50) indicated high levels resistance. RRKC50 with α-cypermethrin varied from 10 100 among indicating Most had...
The mosquito Aedes aegypti is the major vector of four serotypes dengue virus (DENV1–4). Previous studies have shown that Ae. in Mexico a high effective migration rate and gene flow occurs among populations are up to 150 km apart. Since 2000, pyrethroids been widely used for suppression cities Mexico. In Yucatan State particular, applied around case households creating an opportunity local selection evolution resistance. Herein, we test evidence adaptation by comparing patterns variation 27...
Background ZIKV is a new addition to the arboviruses circulating in New World, with more than 1 million cases since its introduction 2015. A growing number of studies have reported vector competence (VC) Aedes mosquitoes from several areas world for transmission. Some used World disparate regions and concluded that these variable but relatively low Asian lineage ZIKV. Methodology/Principal findings Ten aegypti (L) three Ae. albopictus (Skuse) collections made 2016 throughout Mexico were...
Aedes aegypti control programs require more sensitive tools in order to survey domestic and peridomestic larval habitats for dengue other arbovirus prevention areas. As a consequence of the COVID-19 pandemic, field technicians have faced new occupational hazard during their work activities surveillance control. Safer strategies monitor populations, addition minimum householder contact, are undoubtedly urgently needed. Drones can be part solution urban rural areas that dengue-endemic....
We analyzed 790 Aedes aegypti from 14 localities of Mexico in 2009 to update information on the frequency Ile1016 allele voltage-gated sodium channel gene that confers resistance pyrethroids and DDT. The mutation was present all 17 collections, close fixation Acapulco (frequency = 0.97), Iguala (0.93), San Nicolas (0.90). Genotypes at 1016 locus were not Hardy-Weinberg proportions collections Panuco, Veracruz, Cosoleacaque, Coatzacoalcos, Tantoyuca, Monterrey due every case an excess...
Resistance to the insecticides deltamethrin and malathion enzymes associated with metabolic resistance mechanisms were determined in four field populations of Aedes aegypti (L.) from western Venezuela during 2008 2010 using bottle assay microplate biochemical techniques. For deltamethrin, mortality rates after 1 h exposure a 24-h recovery period calculate 50% knock-downconcentration (KC50) lethal concentration (LC50), respectively. malathion, was recorded at 24 determine LC50. ratios...
Permethrin resistance is widespread in Aedes aegypti (L.), the main dengue, zika, and chikungunya virus vector Latin America Caribbean. A common mechanism of to pyrethroids-knockdown (kdr)-is conferred through mutations insect's voltage-dependent sodium channel. In this mosquito, around 10 replacement substitutions voltage-gated channel gene (vgsc) have been reported pyrethroid-resistant strains. Two these mutations, named Ile1,016 Cys1,534, are mosquito populations from This study assessed...
Abstract BACKGROUND The control of Aedes aegypti (L.), the main urban vector that causes arboviral diseases such as dengue, Chikungunya and Zika, has proved to be a challenge because rapid increase in insecticide resistance. Therefore, adequate monitoring resistance is an essential element Ae. it transmits. We estimated frequency intensity (Resistance Frequency Rapid Diagnostic Test [F‐RDT] Resistance Intensity [I‐RDT]) pyrethroid populations from Mexico using bottle bioassay results were...
Abstract Aedes aegypti (L.) is the primary vector of viruses that cause dengue, Zika, and chikungunya, for which effective vaccines drugs are still lacking. Current strategies suppressing arbovirus outbreaks based on insecticide use pose a challenge because rapid increase in resistance. The widespread excessive pyrethroid-based insecticides has created large selection pressure kdr-type resistance, caused by mutations para gene voltage-gated sodium channel (vgsc). Our objective was to...
Insecticide resistance is a serious problem for vector control programmes worldwide. Resistance commonly attributed to mutations at the insecticide's target site or increased activity of detoxification enzymes.We determined knockdown concentration (KC50) and lethal (LC50) deltamethrin in six natural populations adult Aedes aegypti from southeastern Mexico. These were then selected over five generations using LC50 preceding generation that underwent selection, heritability was quantified. For...
Aedes aegypti is a mosquito that transmits viral diseases such as dengue, chikungunya, Zika, and yellow fever. The insect's microbiota recognized for regulating several biological processes, including digestion, metabolism, egg production, development, immune response. However, the role of bacteria involved in insecticide susceptibility has not been established. Therefore, objective this study was to characterize resident field population A. evaluate its associated with insecticides...
The head louse Pediculus humanus capitis (De Geer) is a hematophagous ectoparasite that inhabits the human scalp. Infestations by this insect are commonly known as pediculosis, which more common in younger groups. These infestations asymptomatic; however, skin irritation from scratching occasionally may cause secondary bacterial infections. In recent years, prevalence of pediculosis has increased children; increase been attributed to resistance insecticides used control measure for...
Abstract BACKGROUND Knockdown resistance is conferred primarily by non‐synonymous mutations that reduce pyrethroids binding to voltage‐gated sodium channels. In 2014, kdr mutation V1016I in Aedes aegypti populations resistant was reported for the first time Colombiα, 2016 another mutation, F1534C, and 2018 V419L. Nine of A. , previously characterized as being λ‐cyhalothrin, deltamethrin, cyfluthrin permethrin, were used this study. Genomic DNA determine genotypes allele‐specific PCR their...
Abstract BACKGROUND Resistance to insecticides through one or several mechanisms has a cost for an insect in various parameters of its biological cycle. The present study evaluated the effect deltamethrin on detoxifying enzymes and population Aedes aegypti selected 15 generations. enzyme activities alpha‐ beta‐esterases, mixed‐function oxidases glutathione‐S‐transferases were determined during selection, along with parameters. RESULTS Overexpression as mechanism metabolic resistance was...
Resistance to the organophosphate insecticide chlorpyrifos was evaluated in females from six strains of Aedes aegypti (L.) that expressed high levels cross-resistance eight pyrethroid insecticides. Relative LC50 and LC90 at 24 h a susceptible New Orleans (NO) strain, three were highly resistant (Coatzacoalcos, resistance ratio [RRLC90] = 11.97; Pozarica, RRLC90 12.98; Cosoleacaque, RRLC50 13.94 17.57), one strain moderately (Veracruz, 5.92), two (Tantoyuca Martinez de la Torre, < 5) bottle...