To assist polarized-light spectroscopy for protein-structure analysis, the UV spectrum of p-cresol, chromophore tyrosine, was studied with respect to transition moment directions and perturbation by solvent environment. From linear dichroism (LD) spectra p-cresol aligned in stretched matrices poly(vinyl alcohol) polyethylene, lowest π-π* (Lb) is found have pure polarization over its entire absorption (250-300 nm) a perpendicular symmetry axis (C1-C4), both polar nonpolar environments. For...

Hoechst 33258 binds with high affinity into the minor groove of AT-rich sequences double-helical DNA. Despite extensive studies this and analogous DNA binding molecules, there still remains uncertainty concerning interactions when multiple ligand molecules are accommodated within close distance. Albeit not direct concern for most biomedical applications, which at low drug concentrations, interaction higher important as they can give fundamental insight mechanisms specificity, including...

Despite the extensive interest in structurally explaining photophysics of DNA-bound [Ru(phen)(2)dppz](2+) and [Ru(bpy)(2)dppz](2+), origin two distinct emission lifetimes pure enantiomers when intercalated into DNA has remained elusive. In this report, we have combined a photophysical characterization with detailed isothermal titration calorimetry study to investigate binding Δ Λ both complexes [poly(dAdT)](2). We find that model different geometries, proposed be symmetric canted...

Additive manufacturing (AM) is a rapidly expanding new technology involving challenges to occupational health. Here, metal exposure in an AM facility with large-scale metallic component production was investigated during two consecutive years preventive actions between.Gravimetric analyzes measured airborne particle concentrations, and filters were analyzed for content. In addition, concentrations of particles <300 nm investigated. Particles from recycled powder characterized. Biomonitoring...

A large variety of proteins involved in inflammation, coagulation, lipid-oxidation and lipid metabolism have been associated with high-density lipoprotein (HDL) it is anticipated that changes the HDL proteome implications for multiple functions HDL. Here, SELDI-TOF mass spectrometry (MS) was used to study dynamic protein composition a human experimental low-dose endotoxemia model. Ten healthy men low cholesterol (0.7+/-0.1 mmol/L) 10 high levels (1.9+/-0.4 were challenged endotoxin (LPS)...

We present recent developments on the use of self-assembly methods to bridge gap between sub-nanometer and micrometer length scales.

Human RAD51 protein (HsRad51) catalyses the DNA strand exchange reaction for homologous recombination. To clarify molecular mechanism of in vitro being more effective presence Ca 2+ than Mg , we have investigated effect these ions on structure HsRad51 filament complexes with single- and double-stranded DNA, intermediates. Flow linear dichroism spectroscopy shows that two ionic conditions induce significantly different structures HsRad51/single-stranded complex, while HsRad51/double-stranded...

Biosensors, in which binding of ligands is detected through changes the optical or electrochemical properties a DNA layer confined to sensor surface, are important tools for investigating interactions. Here, we investigate if conformational induced surface-attached molecules upon ligand can be monitored by quartz crystal microbalance with dissipation (QCM-D) technique. duplexes containing 59–184 base pairs were formed on QCM-D crystals stepwise assembly synthetic oligonucleotides designed...

Backgrounds Occurrence of airway irritation among industrial metal workers was investigated. The aims were to study the association between exposures from water-based working fluids (MWF) and health outcome personnel, assess potential effects on proteome in nasal mucous membranes, evaluate preventive actions. Methods prevalence symptoms related work examined 271 metalworkers exposed MWF 24 not at same factory. At time, air levels potentially harmful substances (oil mist, morpholine,...

The Swi5-Sfr1 heterodimer protein stimulates the Rad51-promoted DNA strand exchange reaction, a crucial step in homologous recombination. To clarify how this accessory acts on we have analyzed structure of primary reaction intermediate, Rad51/single-stranded (ssDNA) complex filament formed presence ATP, is affected by Swi5-Sfr1. Using flow linear dichroism spectroscopy, observe that nucleobases ssDNA are more perpendicularly aligned to axis Swi5-Sfr1, whereas bases randomly oriented absence...

Patch Testing with Isobornyl Acrylate in 16 Swedish Patients Contact Dermatitis from Glucose Sensors and/or Insulin Pumps

RAD51 is the key component of homologous recombination pathway in eukaryotic cells and performs its task by forming filaments on DNA. In this study we investigate physical properties formed DNA using nanofluidic channels fluorescence microscopy. Contrary to bacterial ortholog RecA, forms inhomogeneous long vitro, consisting several protein patches. We demonstrate that a permanent "kink" filament where two patches meet if stretch naked between short. The kinks are readily seen present...

Objective: Rheumatoid arthritis (RA) is an inflammatory disease with unknown etiology. This study examines if silica dust exposure increases the risk for seropositive and seronegative RA. Methods: A nationwide registry case–control was conducted that included all cases of RA in Sweden between 2005 2016. In total, 31,139 two matched controls were included. JEM used to estimate exposure. Results: Silica associated a statistically significant increase odds ratio (OR) (OR 1.22, 95% CI 1.05 1.40)...

Abstract Introduction Disinfection by-products are produced in water disinfected with chlorine-based products. One such group is trihalomethanes, and chloroform the most abundant trihalomethane swimming pool areas. Chloroform can be absorbed by inhalation, ingestion, dermal absorption, classified as possibly carcinogenic. Aim To investigate if concentrations air affect concentration urine samples of exposed workers. Methods Workers from 5 adventure indoor pools carried personal samplers...

Exposure to metalworking fluid has been shown cause health problems among workers. The aim of this study was compare outcomes and levels exposure workers exposed before after the implementation preventive measures. frequencies self-reported symptoms, as well concentrations measured substances were lower Logistic regression showed statistically significant differences in report irritations, a stuffy or runny nose, eye irritation, hoarse dry throat, cough with odds ratios 0.31 (95% confidence...

Respiratory syncytial virus (RSV) is the most common cause of severe respiratory tract infection in infants. The aim was to identify host defence components nasopharyngeal aspirate (NPA) from infants with RSV and study expression novel 25 kDa innate immunity protein SPLUNC1.NPAs were analyzed 2-DE MS a pilot study. levels SPLUNC1 immunoblotting 47 NPAs, admitted for diagnosis.Totally, 35 proteins identified NPA, including several such as group X phospholipase A(2) , different S100 SPLUNC1....

Objective To determine whether occupational exposure to silica dust causes an increased risk of developing Crohn’s disease (CD) and ulcerative colitis (UC). Design Case–control study CD (K50) UC (K51) from 2007 through 2016. Controls were matched cases (2:1) based on age, sex county at the time diagnosis. A job matrix was used estimate all controls. Setting Medical data National Outpatient Register implement a case–control analysis, while two controls for each case selected Total Population....

Objective: To study occupational exposure totrichloramine and endotoxins in air at adventure rehabilitation swimming pool facilities from an adverse health effects perspective. Methods: Air concentrations of trichloramine were measured five 10 facilities. Respiratory ocular symptoms self-reported, spirometry fraction exhaled nitric oxide (FE no ) measured. Results: Compared to facilities, the mean higher, both personal (80 μg/m 3 ( n = 41) vs 19 21)) stationary (183 51) 23 32)), with higher...

Upper airway irritation is common among individuals working in moldy and damp buildings. The aim of this study was to investigate effects on the protein composition nasal lining fluid. prevalence symptoms relation work environment examined 37 two Microbial growth confirmed one Nasal lavage fluid collected from 29 exposed subjects 13 controls, not a building. Protein profiles were investigated with proteomic approach evaluated by multivariate statistical models. Subjects both workplaces...

Objective: This study was to investigate the acute effects of hand-arm vibrations on nerve functions hands, and impact grip force applied vibrating tool during exposure. Methods: Grip strength perception vibration, touch, temperature were evaluated using quantitative sensory testing (QST) before after vibration exposure in 21 occupationally unexposed individuals. The procedure performed twice, with a higher being second occasion. Results: Vibration significantly impaired both exposures....

Abstract Introduction Chloroform is found in swimming pool air and can be absorbed by inhalation, ingestion dermal absorption, classified as possibly carcinogenic. The study aimed to investigate if chloroform concentrations water affect the concentration urine samples of exposed workers. Methods Workers from five adventure indoor pools carried personal samplers provided up four during one workday. were analysed with a linear mixed model analysis possible correlation between concentrations....