Phospholipase A2 is the most abundant venom gland enzyme, whose activity leads to activation of inflammatory response by accumulating lipid mediators. This study aimed identify, classify, and investigate properties PLA2 isoforms. Then, present findings were confirmed chemically measuring PLA2. The sequences representing annotation extracted from Androctonus crassicauda transcriptome dataset using BLAS searches against local database. We found several cDNA classified named conducting multiple...

Abstract Background The Androctonus crassicauda , belonging to the genus of family Buthidae, is most venomous scorpion in Middle East countries. However, venom gland transcriptome profile A. has not yet been studied. In this study, we elucidated and compared gene expression profiles adult juvenile male using high-throughput sequencing. This first report transcriptional analysis glands scorpions different growth stages, with insights into identification key genes during development. Results A...

Venom hyaluronidase is an enzyme belonging to the glycoside hydrolase family that plays a critical role in systemic envenomation by spreading toxins tissue and destruction of extracellular matrix. The roles venom during have been widely studied, but identification its variants isoforms still under investigation. In this study, we developed filtering method identify exon-intron pattern, alternative splicing events A. crassicauda H. lepturus scorpions using RNA-seq technique. Furthermore,...

Scorpion venom is a rich source of biological active peptides and proteins. Transcriptome analysis the gland provides detailed insights about peptide protein components. Following transcriptome different species in our previous studies, research team has focused on

Abstract The potential function of long non-coding RNAs in regulating neighbor protein-coding genes has attracted scientists’ attention. Despite the important role lncRNAs biological processes, a limited number studies focus on non-model animal lncRNAs. In this study, we used stringent step-by-step filtering pipeline and machine learning-based tools to identify specific Androctonus crassicauda analyze features predicted scorpion 13,401 were detected using A. transcriptome. blast results...

Hydatidosis is an important zoonosis caused by a parasitic tapeworm, namely Echinococcus granulosus. This infection distributed worldwide and affects the health as well economic loss in both humans animals. In most cases, disease needs chemotherapy with or without surgery. Conventional drugs have some major problems, including drug complications, harmful side effects, also progressive resistance. According to importance of biological productions alternative medicine, large number studies...

The Androctonus crassicuda is the most diverse scorpion species in family of Buthidae, which endemic to Khuzestan province, Iran. Investigation relationship by means a molecular study specimens one new approaches due limitations morphological approaches. In current study, analysis was based on 32 characteristics A. native southwest Moreover, DNA sequencing two mitochondrial markers, namely cytochrome oxidase subunit I and 12sRNA loci performed, phylogenetic tree constructed using maximum...

Background: Hemiscorpius lepturus is the most critical scorpion in Khuzestan, Iran, responsible for majority of deaths caused by stings. Objectives: This prospective randomized experimental study was performed from June to August 2019 investigate effects various venom fractions on hemostatic system. Methods: Lyophilized H. separated into six Sephadex G-50 gel chromatography. A total 96 Albino male rats were treated eight equal groups; Control: 0.5 ml normal saline (IP); Whole group: venom,...

Background: Anaplasma sp. is a blood protozoon that causes economic damage to the livestock industry. Therefore, studying this disease’s epidemiology and distribution pattern in different regions essential. Objectives: This study aimed investigate variety of infections sheep population Khuzestan Province Iran. Methods: A total 200 samples were randomly collected examined using specific nested polymerase chain reaction (nPCR) based on 16S rRNA gene. Results: The prevalence phagocytophilum was...

Recently, RNA sequencing has been widely applied to deeply understand the molecular diversity of venom compounds various venomous animal species, including scorpions. Among scorpion species Buthidae family, there are many documents stinging and severe envenoming victims by Androctonus crassicauda. We present here a high-throughput dataset glands from five A. crassicauda individuals, male female Furthermore, assembled data corresponding annotated PLA2 transcripts also presented. The in this...

Background: Scorpion stings are responsible for many deaths in humans; however, the toxicity mechanisms of venoms from species not well studied. We investigated cardiotoxicity crude venom H. lepturus scorpion and its isolated fractions, F-I to F-VI. Methods: The scorpion’s was extracted into six fractions by chromotagraphy. Healthy male Wistar rats (N=72) were equally divided eight groups nine: G1: Controls (0.5ml. normal saline), G2: Crude (1000µg/kg), G3: (120µg/kg), G4: F-II (430µg/kg),...

Giardia duodenalis is globally recognized as an important zoonotic intestinal protozoan parasite. So far, eight assemblages of G. (A-H) have been identified. Substantial evidence suggests the potential A, B, and E in livestock. In this study, genotype isolates was genetically identified by determining sequence ssu-rRNA gene performing polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) on glutamate dehydrogenase parasite Arabian horses from Ahvaz, located southwest...

BACKGROUND: Mesobuthus eupeus is an indigenous scorpion species in Southwest Iran which respon- sible for the majority of sting cases Khuzestan province. OBJECTIVES: The effects M. venom were investigated on chief metabolic hormones rats. METHODS: A total 45 Albino male rats divided into 3 equal groups: group 1 (control): 0.5 ml normal saline was administered intraperitoneally (IP); 2 and 3: with a dose mg/kg IP, respectively. Sampling performed at 8, 24, 48 hours after venom/ injection....

Background: The present study determined the effect of fractions obtained from Hemiscorpius lepturus scorpion venom on tachyzoite Toxoplasma gondii. Methods: dried He. Khuzestan Province, southern Iran in 2019 were isolated through gel filtration chromatography, and then tachyzoites exposed to at different concentrations. Trypan blue counting MTT applied assay viability, inhibition cellular growth Vero cells was evaluated. Results: maximum observed fraction 5 venom. To further separate...