The S1 genome segment of avian reovirus strain S1133 was cloned and completely sequenced. sequence comprised 1636 bp with three distinct open reading frames (ORFs), suggesting the gene polycistronic in nature. ORFs from 5′ to 3′ were predicted encode polypeptides 9.8, 3.8 34.9 kDa, respectively. Of ORFs, only third possessed AUG initiation codon an optimum context for translation. ORF-encoded protein, 326 amino acids length, expressed Escherichia coli used as antigen immunoblots. protein...

To evaluate the prevalence of infectious bronchitis virus (IBV) type 4/91 in Iran, tracheal swabs from 77 broiler flocks 16 provinces were collected at slaughterhouse. Swabs subjected to RNA extraction and tested by RT-PCR, followed a type-specific nested PCR. The viral was detected 33 samples (42.8%) different provinces. results indicate relatively high IBV Iran necessitate revising vaccination programme against this disease.

A simple colorimetric assay is presented for detecting bovine viral diarrhea virus (BVDV)-RNA based on aggregation of gold nanoparticles (AuNPs) in the presence charge-neutral peptide nucleic acids (PNA). Free PNA oligomers tended to be adsorbed onto AuNPs and act as a coagulant, whereas hybridizing complementary RNA with disrupted PNA-induced AuNP aggregation, NPs remained stable. However, non-complementary did not have this effect, induced that resulted color change reaction from red blue....

It has been suggested that avian reovirus σ3 protein is analogous to σ1 trimer, the mammalian attachment protein. We have investigated multimeric nature and cell binding ability of The data presented here demonstrate a multimer in its undisrupted form as determined by SDS-PAGE non-dissociating conditions. However, virion-associated COS-7 cell-expressed behaved differently SDS-PAGE, suggesting need for virus-associated factor(s) control multimerization also show Escherichia coli expressed...

Infectious Pancreatic Necrosis Virus (IPNV) is the causal agent of a highly contagious disease that affects many species fish and shellfish. This virus causes economically important diseases farmed rainbow trout, Oncorhynchus mykiss, in Iran which often associated with transmission pathogens from European resources. In this study, moribund trout fry were collected during an outbreak IPNV three different farms one northern province (Mazandaran), two west provinces (Chaharmahal Bakhtiari,...

Abstract Background Widely regarded as one of the chief causes diseases in cattle population, bovine herpesvirus‐1 (BoHV‐1) has potential to infect sheep and goat, making them reservoirs or hosts for this virus. Thus, preventive measures against BoHV‐1 should not overlook ability virus other animals. Aims Therefore, focal point study was ascertain seroprevalence 300 healthy goats, relationship between host environmental determinants infection, contributing role goats epidemiology BoHV‐1....

Summary Monoclonal antibodies (MAbs) were produced against a vaccinal S1133 strain of avian reovirus. Characterization six MAbs in Western blotting, radioimmunopre‐cipitation and gold immunoelectron microscopy revealed that the specific to outer capsid proteins, μ2/μ2c, σ2 σ3. Two three MAbs, directed protein, neutralized virus infectivity broadly manner, whereas third had no neutralizing activity. The only MAb which reacted with σ3 protein showed type‐specific recognizing μ2/μ2c proteins...

Cervical cancer is one of the important reasons mortality among females. Prevention, early diagnosis and immediate treatment can affect rate in this several epidemiological studies have shown a strong relationship between human papilloma viruses (HPVs) cervical cancer.The present study was conducted to survey HPV infections women population with dysplasia/metaplasia southwest Iran.72 paraffin-embedded biopsies which had been previously archived from dysplasia were examined by polymerase...

Abstract Background and Aims: Akabane disease causes epidemics of abortions, stillbirths congenital malformations manifested as arthrogryposis hydranencephaly or microanencephaly in sheep, goats cattle. virus replicates arthropods is transmitted by either mosquitoes midges. Outbreaks the have been reported from many countries Southeast Asia, Middle East, Africa Australia. The aim this study was to evaluate prevalence infection correlation with host environmental determinants sheep Khouzestan...

Fifty rotavirus-positive feacal samples, selected from 500 ELISA tested diarrheic specimens were used in this study. Viral RNA was extracted each sample and reveres transcribed to cDNA. The cDNA then amplified by oligonucleotide primers specific for segment 9, coding VP7. After the first amplification, PCR products subjected a multiplex semi-nested investigate presence of bovine rotavirus serotypes: G6, G8 G10. results indicated prevalence 48 26% G6 G10 serotypes, respectively. Twenty four...

Bovine herpesvirus type-1 (BoHV-1) is a DNA virus that classified in the family Herpesviridae, subfamily Alphaherpesvirinae and genus Varicellovirus. This causes respiratory disease, abortion, conjunctivitis, infectious pustular vulvovaginitis or balanoposthitis other clinical forms of complex diseases. The aim this study was to evaluate seroprevalence risk factors BoHV-1 infection cattle Khuzestan province Iran. Blood samples were randomly collected from 534 9 districts including Ahvaz,...

Scorpion venom is important and rich source of peptides, most which have been widely used as pharmacological tools for unraveling structure-function relationship various ion channels. Naturally occurring toxins can be also considered lead compounds in the development novel drugs.In this context, scorpion-derived peptide neurotoxins specific to sodium channels shown promise potential therapeutic targets treatment human diseases.A cDNA library from extracted RNA was constructed using RT-PCR...

Summary To develop an ELISA technique for the detection of antibodies against Babesia ovis, infected erythrocytes were lysed and supernatant soluble antigen, after sonication ultracentrifugation lysate was used as antigen. Optimal dilution antigen determined by checkerboard titrations, using positive negative control sera. A correlation 85% observed between results developed IFA techniques. study seroprevalence ovis in Khouzestan province, south Iran, a total 1000 sheep sera collected from...

Background: Although Escherichia coli (E. coli)is a part of intestinal normal microflora warm-blooded animals, includingpoultry, outbreaks occur in poultry raised below standard sanitation and duringthe course respiratory or immunosuppressive diseases. Avian pathogenic E.coli (APEC) harbors several genes associated with virulence andpathogenicity. APEC strains are responsible for some diseases poultryincluding colibacillosis, swollen head syndrome, yolk sac infection, omphalitisand...

Bovine herpesvirus 1 (BoHV-1) is one of the most important infectious viruses cattle which considered as causative agent several clinical conditions in this species. Hence, we tried to isolate a local strain virus from carrier Ahvaz based on strategy for reactivation latent infection hosts having antibodies against virus. Thus, 20 were examined identify seropositive animals using neutralization test (VNT). Afterward, 4 out 8 selected and experimentally stressed by daily injection...

Background: Small ruminant morbillivirus (SRMV), bovine viral diarrhea virus (BVDV), herpes virus-1 (BHV-1), and ephemeral fever (BEFV) are among the most important viruses of farm animals. Objectives: This study aimed at serological detection SRMV, BVDV, BHV-1, BEFV in camel population Khuzestan Province, located southwest Iran. Methods: A total 150 blood samples were randomly collected from free-ranging, seemingly healthy camels both sexes various ages 8 different regions The sera tested...

Summary Tetanus is a disease caused by tetanus toxin, potent inhibitor for the release of inhibitory neurotransmitter in central nervous system that causes spastic paralysis. Fragment C (52 kD) this toxin responsible binding to neuronal membrane. For reason, and also its non toxigenic immunogenic nature, fragment might be ideal new vaccine development. Presently, with respect incidence neonates animals side effects toxoid vaccine, designing more effective efficient prevention crucial. A...

The aim of this study was to investigate the immunogenicity a plasmid deoxyribonucleic acid (DNA) vaccine encoding G1 epitope bovine ephemeral fever virus (BEFV) G glycoprotein in mice. A DNA carrying gene constructed and designated as pcDNA3.1-G1. expression target confirmed human embryonic kidney 293 (HEK 293) cells transfected with pcDNA3.1-G1 by indirect immunofluorescent staining. Immunisation experiments were intramuscularly carried out vaccinating 6-week-old female mice four groups,...