Abstract Background Urinary Tract Infection (UTI) is one of the most common bacterial infectious diseases which causes considerable morbidity and costly health problems. Uropathogenic Escherichia coli (UPEC), pathogen causing UTI, a highly heterogeneous group extraintestinal pathogenic E. (ExPEC) may carry variety virulence factors belonging to different phylogenetic backgrounds. The current study aimed investigate frequency association between various (VFs) groups UPEC commensal isolates....

In this study, we evaluated the antiviral activity of gold nanoparticles (AuNPs) against foot-and-mouth disease virus (FMDV), that causes a contagious in cloven-hoofed animals. The anti-FMDV AuNPs was assessed using plaque reduction assay. MTT assay used for quantitatively measuring cytopathic effect caused by viral infection. 50% cytotoxicity concentration measured and found to be 10.4 μg/ml. yield showed AuNP have an approximately 4-fold titer compared with controls. Plaque at...

Introduction . The main aetiological agent of urinary tract infection (UTI) is Escherichia coli , categorized as uropathogenic E. (UPEC). genome UPEC shows a high degree plasticity, which leads to the emergence ‘intermediary strains’ with different traits from parental pathotypes. Gap Statement/Aim We aimed assess frequency and types hybrid among isolates causing UTI characterize virulence properties these molecularly phenotypically. Methodology After detection intestinal pathogenic (IPEC)...

ABSTRACT We report the draft genome sequence of an extensively drug-resistant and metallo-beta-lactamase-producing Escherichia coli Ne7 strain isolated from a urinary tract infection patient. Under O8:H9 serotype F phylogenetic group, this had type ST648, five replicon plasmids, 16 virulence genes, 21 antibiotic resistance genes.

Nowadays natural products such as pure compounds and plant extract scan provide unlimited opportunities for new antiviral drugs. Newcastle disease virus (NDV) is one of the most important viral diseases in poultry industry. Vaccination could protection against NDV outbreaks, but it not sufficient because infections by NDVs have remained frequent around world.The current research aimed to study Achillea millefolium Thymus vulgaris activity (NDV).The plants was measured reduction assay titer,...

Foot-and-mouth disease (FMD) is an extremely contagious viral of cloven-hoofed animals that can lead to huge economic losses in the livestock production. No antiviral therapies are available for treating FMD virus (FMDV) infections animals. The effects magnesium oxide nanoparticles (MgO NPs) on FMDV were investigated cell culture. viability cells after MgO NP treatment was determined using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. direct NPs extracellular...

Introduction: Diarrheagenic Escherichia coli (DEC) is a major etiologic agent among the pathogens that cause diarrhea in children.
 Methodology: To investigate presence and pathotypes of DEC children under five years age, living province Khouzestan, Iran. 208 stool samples were screened by multiplex-PCR. The isolated isolates investigated for resistance to various antimicrobials including production extended-spectrum beta-lactamases (ESBLs) phylogenetic groups determined.
 Results:...

Background: Urinary tract infection (UTI) is a common bacterial disease which may cause chronic renal failure and hypertension. Many reports suggest that the rate of antibiotic resistance to infectious organisms increasing. Objectives: This study aimed detect also compare frequency drug pattern Gram negative bacteria isolated from patients with community-acquired UTIs in Isfahan. Patients Methods: In this cross-sectional descriptive study, 702 samples 476 females 226 males referred medical...

A simple colorimetric assay is presented for detecting bovine viral diarrhea virus (BVDV)-RNA based on aggregation of gold nanoparticles (AuNPs) in the presence charge-neutral peptide nucleic acids (PNA). Free PNA oligomers tended to be adsorbed onto AuNPs and act as a coagulant, whereas hybridizing complementary RNA with disrupted PNA-induced AuNP aggregation, NPs remained stable. However, non-complementary did not have this effect, induced that resulted color change reaction from red blue....

Avian pathogenic Escherichia coli (APEC) is an important bacterial pathogen that causes avian colibacillosis and leads to huge economic losses in the poultry industry. Different virulence traits contribute pathogenesis of APEC infections, antimicrobial resistance (AMR) has also been overwhelming issue worldwide. In present study, we aimed investigate compare presence virulence-associated genes (VAGs), AMR, phylogenetic group's distribution among fecal E. (AFEC) strains. from birds with...

Aim: We aim to use peptide nucleic acid (PNA) for antisense therapy against bovine viral diarrhea virus (BVDV), a surrogate model of human hepatitis C virus, and introduce an optimal approach delivering PNA into the cell. Materials & methods: was designed hybridization 5′-untranslated region BVDV RNA in order form heteroduplex structure inhibit translation replication virus. Gold nanoparticles (AuNPs) were used as delivery system PNA. Results: The cellular uptake PNA-AuNPs inhibition...

To determine the presence of some toxins diarrheagenic Escherichia coli (DEC) in uropathogenic E. (UPEC), 138 urinary tract infection (UTI)-causing UPECs were analyzed. The astA, set, sen and cdtB genes detected 13 (9.4%), 2 (1.3%), (9.4%) 0 (0%) UPEC isolates respectively. results show that encoding can be transferred from DEC pathotypes to therefore these transform into potential diarrhea-causing agents.

Abstract Background Uropathogenic Escherichia coli (UPEC) is a major cause of urinary tract infection (UTI); however, treatment UTI has been challenging due to increased antimicrobial resistance (AMR). One the most important types AMR carbapenem (CR). CR bacteria are known as an threat global public health today. Class B metallo-beta-lactamases (MBLs) one factors for against carbapenems. We aimed investigate characteristics UPEC isolates producing MBL. Methods A cross-sectional study was...

Abstract Background Bovine viral diarrhea virus (BVDV) is a major economic disease that has been spread in most countries. In addition to vaccination, one of the main ways control and prevent it from spreading detect cull infected animals, especially those with persistent infection (PI). We developed compared two colorimetric biosensor assays based on probe-modified gold nanoparticles (AuNPs) BVDV. Specific probes were designed 5′ untranslated region BVDV-RNA. The thiolated immobilized...

Abstract Background Bovine ephemeral fever (BEF) is an arthropod-borne viral disease caused by the BEF virus (BEFV). This single-stranded RNA that affects cattle and water buffalo endemic in tropical subtropical regions including Iran. While a major of Iran, information regarding its agent, molecular characterization, circulating viruses are highly limited. The current study aimed to, firstly, determine genetic antigenic characteristics BEFV strains Khuzestan province Southwest Iran 2018...

The enteroaggregative Escherichia coli (EAEC) has been one of the most intriguing emerging bacterial pathogens in children that occur both developing countries and industrial world. Although various phenotypic genotypic based protocols have suggested for diagnosis EAEC, they are not conclusive or practical to be used clinical laboratories.In this study, we analyzed compared 36 typical EAEC strains (aggR-positive) by methods.Briefly, pCVD432 was detected all isolates along with aggR, then it...

Abstract Background The emergence of metallo-β-lactamase (MBL)-producing isolates is alarming since they carry mobile genetic elements with great ability to spread; therefore, early detection these isolates, particularly their reservoir, crucial prevent inter- and intra-care setting dissemination establish suitable antimicrobial therapies. current study was designed evaluate the frequency resistance (AMR), MBL producers identification genes in Escherichia coli strains isolated from fecal...