The E2F family of transcription factors regulates genes involved in various aspects the cell cycle. Beyond well-documented role G1/S transition, mitotic regulation by has also been reported. Proper progression is monitored spindle assembly checkpoint (SAC). SAC ensures bipolar separation chromosomes and thus prevents aneuploidy. There are limited reports on E2F. Our previous work identified protein Cdc20 as a novel transcriptional regulator ubiquitin carrier UbcH10. However, none complex...

The study was conducted aiming at the isolation and identification of Salmonella Escherichia coli (E. coli) from different brands poultry feeds sold in Savar, Dhaka, Bangladesh. Seven were subjected to microbiological analysis. All these samples analyzed by culturing media such as nutrient broth (NB), Agar (NA), SS (Salmonella-Shigella Agar), BGA (brilliant-green Mac Conkey, DHL EMB (eosin methylene blue) media. Total bacterial colonies all counted separately on Hence, bacteria 9.5×105 feed...

The ongoing usage of natural products since time immemorial has resulted in several beneficial medications derived from plant sources. Flavonoids are well known for their anti-bacterial role against a wide range micro-organisms. Among them Mycobacterium tuberculosis (Mtb) is the bacterial agent, which to cause disease. Tuberculosis considered as worldwide health issue causing morbidity and mortality every year. Due many challenges faced by current treatment regimen, there need new...

The advent of Multi Drug Resistant (MDR) strain Mycobacterium tuberculosis (TB) necessitated search for new drug targets the bacterium.It is reported that 3.3% all cases had multidrug resistance (MDR-TB) in 2009 and each year, about 0.44 million MDR-TB are estimated to emerge 0.15 people with die.Keeping such an alarming situation under consideration we wanted design suitable anti tubercular molecules target using computational tools.In work Methionine aminopeptidase (MetAP) was considered...

Aims: To modify a strain of Salmonella serotype Typhimurium to express unique marker traits and then define how the concentration in bovine faeces affects probability its detection by culture preceded immunomagnetic separation (IMS). Methods Results: DNA encoding for production green fluorescent protein (gfp) resistance kanamycin was inserted into bacterial chromosome Salm. Typhimurium. Transposon insertion demonstrated Southern blot hybridization. Varying amounts one electroporant...

The advent of carbapenem resistance by the production β-lectamases and mutated penicillin binding proteins (PBPs) has challenged treatment Enterobacteriaceae. Hence there is an urgent need to establish drugs that can fit in pipeline overcoming those situations. working hypothesis work based on two facts, i.e., i) design inhibitors against PBPs which present cannot bind efficiently kill pathogen inhibiting cell wall formation, ii) molecules with high affinity, so they supplement available...

Cervical cancer is the second largest form of to infest leading cause death in women worldwide. There are many causes but viruses most common among them. Human papillomaviruses (HPVs) found be causative organism almost 99.7% cases. HPV16 frequent HPV type malignant neoplastic growth about 60% cervical carcinoma limited success achieved surgical removal or by immune modulation and more effective therapies under investigation. Observing mortality rate we theorize a need for alternative...

Antibiofilm phenomenon has become a novel area of research for removing deleterious biofilm. In the present study, strains from different environmental sources were tested screening antibiofilm compounds. Crude extracts various microorganisms evaluated through crystal violet assay and growth curve analysis. Characterization compound was performed by pre-coating microtiter plate Cell Surface Hydrophobicity experiment. Among organisms, cell free (5% v/v) Bacillus subtilis S01 inhibited...

The liver is prone to damage induced by xenobiotics, which significantly contributes the widespread prevalence of diseases. To explore potential therapeutic avenues, this in silico study utilizes molecular docking techniques elucidate interactions between bioactive compounds derived from Flacourtia jangomas and their target proteins, particularly androgen receptor (AR) acetyl cholinesterase, both implicated hepatocellular carcinogenesis. While pharmaceutical drugs for diseases exist,...

Mycobacterium tuberculosis (Mtb), due to its unusual organization crosses different immune barriers and causes tuberculosis. The advent of multidrug resistance (MDR-TB) has attained alarming situation. Hence, computational drug design been performed in this work find potent molecules for purpose. Isoniazid is a widely used frontline against But reports justified the inactivity isoniazid on acetylation by Arylamine N-acetyltransferase (NAT). 35 countries were highlighted have from survey...

Urinary tract infections (UTI) caused primarily by uropathogenic Escherichia coli (UPEC) are indeed an extremely contagious disease that affects people all over the world. FimH is a major virulence component in UTI pathogenesis, and inhibiting function can be efficient means to disarm UPEC bacteria, as well crucial target development of non-antibiotic mediated treatment options. The goal this study was identify anthocyanins plant parts assess
 their pharmacological characteristics. A...

Abstract Urinary tract infections (UTIs) are the most common form of nosocomial infection primarily caused by Escherichia coli . Complicated UTIs carries higher risk treatment failure, recurrent and increased morbidity. Methionine aminopeptidase (MetAP) has got tremendous importance as bacterial drug target due to its role in cell growth membrane integrity. However, participation metal chelating residues occurrence enzyme human body complicates process selection suitable inhibitor. This...

Background: Urinary tract infections (UTIs) are the most common form of nosocomial infection primarily caused by Escherichia coli. Complicated UTIs carry a higher risk treatment failure, recurrent infections, and increased morbidity. Methionine aminopeptidase (MetAP) has gained tremendous importance as bacterial drug target due to its role in cell growth membrane integrity. However, participation metal-chelating residues occurrence enzyme human body complicate process selecting suitable...

background: Enzyme UDP-NAG enolpyruvyl transferase (MurA), which aids in the formation of cell walls and is identified as primary target antibiotic Fosfomycin, a significant pharmacological case uropathogenic Escherichia coli (UPEC). Finding potent drug with distinct mode action required since UPEC treatment challenging due to resistance resurrection. methods: Here we have used virtual high-throughput screening molecular dynamics simulation approach explore effectiveness selected novel...

Tuberculosis is a major disease still today. Though instigation of Directly Observed Therapy, Short Course, (DOTS) has been widely implemented, new infection with tuberculosis in 2010-2011 was recorded as 8.8 million. The synergy multidrug resistant strains and HIV epidemics made the treatment difficult necessitated care discovery molecules alternative drug targets. Mycobacterium known for its capacity latent also peculiar mechanism granuloma formation where pathogen multiplies. This process...

Tuberculosis is a major disease still today. Though instigation of Directly Observed Therapy, Short Course, (DOTS) has been widely implemented, new infection with tuberculosis in 2010-2011 was recorded as 8.8 million. The synergy multidrug resistant strains and HIV epidemics made the treatment difficult necessitated care discovery molecules alternative drug targets. Mycobacterium known for its capacity latent also peculiar mechanism granuloma formation where pathogen multiplies. This process...

Background: Diabetes mellitus, a chronic disease, has grown wide concern in today's world. Majority of the world’s population is affected by this disease. All existing drugs have some side effects such as weight gain, risk liver skin problems etc. This situation demands need more and potent drugs. Demand for new faster acting great it necessitated search drug targets. Keeping view we wanted to screen out non-toxic anti-diabetic molecules applying computational tools. In present work Protein...

Urinary tract infection (UTI), mainly caused by uropathogenic Escherichia coli (UPEC), is a dreaded infectious disease globally. FimH key virulence factor in UTI pathogenesis and inhibition of function can be an effective way to disarm the UPEC bacteria act as vital target development non-antibiotic mediated approach treat UTIs. The present study was undertaken identify phytochemicals from cranberry bearberry plant parts evaluate their pharmacological properties. properties those compounds...

Parkinson’s disease (PD) is a progressive neurodegenerative disorder with complex etiology. Autosomal recessive mutations in PARK7/DJ-1 gene have been associated early onset PD. Growing evidence has suggested DJ-1 as putative sensor of oxidative stress. Reduced levels protein reported from the cerebrospinal fluid sporadic PD patients. Several case-control association studies identified g.168_185del variants conferring susceptibility towards pathogenesis. Similarly, among patients eastern...