A5102964635- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Antibiotics Pharmacokinetics and Efficacy
- Neutropenia and Cancer Infections
- Tuberculosis Research and Epidemiology
- Ocular Infections and Treatments
- Infectious Diseases and Tuberculosis
- Escherichia coli research studies
- Bacterial Infections and Vaccines
- Intraocular Surgery and Lenses
- Pneumonia and Respiratory Infections
- Salmonella and Campylobacter epidemiology
- Pneumocystis jirovecii pneumonia detection and treatment
- Enterobacteriaceae and Cronobacter Research
- Ocular Diseases and Behçet’s Syndrome
- Antibiotic Use and Resistance
- Urinary Tract Infections Management
- Mycobacterium research and diagnosis
Universidade Federal de São Paulo
2013-2019
Barcelona Institute for Global Health
2018
We report the microbiological characterization of four New Delhi metallo-β-lactamase-1 (blaNDM-1)-producing Enterobacteriaceae isolated in Rio de Janeiro, Brazil. blaNDM-1 was located on a conjugative plasmid and associated with Klebsiella pneumoniae carbapenemase-2 (blaKPC-2) or aminoglycoside-resistance methylase (armA), 16S rRNA not previously reported Brazil, two distinct strains Enterobacter cloacae. Our results suggested that introduction Brazil has been accompanied by rapid spread,...
The objective of this study was to assess the antimicrobial resistance enteroaggregative
Infections are the major cause of morbidity and mortality in children with cancer. Gaining a favorable prognosis for these patients depends on selecting appropriate therapy, which turn rapid accurate microbiological diagnosis. This study employed real-time PCR (qPCR) to identify main pathogens causing bloodstream infection (BSI) treated at Pediatric Oncology Institute IOP-GRAACC-UNIFESP-Brazil. Antimicrobial resistance genes were also investigated using this methodology.A total 248 samples...
Abstract Background Early identification of pathogens and antimicrobial resistance in bloodstream infections (BSIs) decreases morbidity mortality, particularly immunocompromised patients. The aim the present study was to compare real-time polymerase chain reaction (PCR) with commercial kits for detection 17 from blood culture (BC) 10 genes. Methods A total 160 BCs were taken bone marrow transplant patients screened Gram-specific probes by multiplex PCR genus-specific sequences using TaqMan...
Bloodstream infections (BSIs) are the major cause of mortality in cancer patients. Molecular techniques used for rapid diagnosis BSI, allowing early therapy and improving survival. We aimed to establish whether real-time quantitative polymerase chain reaction (qPCR) could improve paediatric patients, describe predominant pathogens BSI their antimicrobial susceptibility.Blood samples were processed by BACTEC system microbial identification susceptibility tests performed Phoenix system. All...
In order to determine the liquor concentration of fosfomycin, we chose 27 patients who were suffering from meningitis with different etiology. According route, type administration and doses employed, classified into five groups. Blood samples taken 1 h after concluding antibiotic 2 CSF sample. The fosfomycin in serum then determined laboratory. evaluate results divided our cases three groups according state their meningeal inflammation. first group active meningitis, obtained an average...
Tuberculous meningitis (TBM) is a severe form of extrapulmonary tuberculosis. The aims this study were to evaluate in-house molecular diagnostic protocols DNA extraction directly from CSF samples and the targets amplified by qPCR as an accurate fast diagnosis TBM. One hundred 68 patients suspected TBM studied. Four techniques (phenol-chloroform-thiocyanate guanidine, silica thiocyanate resin, resin with ethanol) compared used determine best target ( IS6110 , MPB64, hsp65 KDa ) qPCR. protocol...
Bloodstream infections (BSIs) are serious associated with high rates of morbidity and mortality. Every hour delay in initiation an effective antibiotic increases mortality due to sepsis by 7%. Turnaround time (TAT) for conventional blood cultures takes 48 h, forcing physicians streamline therapy exposing patients broad-spectrum antimicrobials. Our objective was (1) evaluate the accuracy TAT optimized workflow combining direct matrix-assisted laser desorption ionization time-of-flight mass...
Febrile Neutropenia represents a medical emergency and the use of appropriate antimicrobial therapy is essential for better outcome. Although being time-consuming, conventional cultures susceptibility tests remain golden standard practices microbiology identification. Final reports are typically available within several days. Faster diagnostic tools, such as species identification trough Matrix Assisted Laser Desorption Ionization-Time Flight (MALDI-TOF) molecular techniques might help to...