A5000042350- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Bacterial biofilms and quorum sensing
- Antibiotic Use and Resistance
- Antibiotics Pharmacokinetics and Efficacy
- Streptococcal Infections and Treatments
- Ocular Infections and Treatments
- Vibrio bacteria research studies
- Pneumonia and Respiratory Infections
- Antifungal resistance and susceptibility
- Ocular Diseases and Behçet’s Syndrome
- Infective Endocarditis Diagnosis and Management
- Healthcare Regulation
- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Escherichia coli research studies
- Infections and bacterial resistance
- Salmonella and Campylobacter epidemiology
- Cervical Cancer and HPV Research
- Enterobacteriaceae and Cronobacter Research
- Neutropenia and Cancer Infections
- Pharmaceutical and Antibiotic Environmental Impacts
- Fungal Infections and Studies
Universidade Federal de São Paulo
2016-2025
Universidade Nove de Julho
2008-2021
Escola Superior de Enfermagem de Lisboa
2021
Union des Industries Ferroviaires Européennes
2015
Fundação de Apoio à Universidade Federal de São Paulo
2008-2012
Novem (Netherlands)
2011
Medicina
2010
Universidade Federal de Ciências da Saúde de Porto Alegre
2008
Hospital São Paulo
1997-2006
All Africa Leprosy Tuberculosis and Rehabilitation Training Centre
2006
To develop a single multiplex real-time PCR assay to detect six different genetic types of carbapenemases already identified in Enterobacteriaceae (KPC, GES, NDM, IMP, VIM and OXA-48). A total 58 bacterial isolates were tested. Thirty previously characterized as resistant carbapenems documented by sequencing analysis carry the following genes: blaKPC type, blaGES blaIMP blaVIM blaOXA-48 blaNDM-1. These positive strains included 21 Enterobacteriaceae, 1 Acinetobacter baumannii 8 Pseudomonas...
Nosocomial bloodstream infections (nBSIs) are an important cause of morbidity and mortality. Data from a nationwide, concurrent surveillance study, Brazilian SCOPE (Surveillance Control Pathogens Epidemiological Importance), were used to examine the epidemiology microbiology nBSIs at 16 hospitals. In our study 2,563 patients with included 12 June 2007 31 March 2010. Ninety-five percent BSIs monomicrobial. Gram-negative organisms caused 58.5% these BSIs, Gram-positive 35.4%, fungi 6.1%. The...
Candidemia is a growing problem in hospitals all over the world. Despite advances medical support of critically ill patients, candidiasis leads to prolonged hospitalization, and has crude mortality rate around 50%. We conducted multicenter surveillance study 16 distributed across five regions Brazil assess incidence, species distribution, antifungal susceptibility, risk factors for bloodstream infections due Candida species. From June 2007 March 2010, we studied total 2,563 nosocomial...
ABSTRACT Metallo-β-lactamase enzymes (MβL) are encoded by transferable genes, which appear to spread rapidly among gram-negative bacteria. The objective of this study was develop a multiplex real-time PCR assay followed melt curve step for rapid detection and identification genes encoding MβL-type based on the amplicon melting peak. reference sequences all IMP VIM types, SPM-1, GIM-1, SIM-1 were downloaded from GenBank, primers designed obtain amplicons showing different sizes peak...
Inappropriate antibiotic use in normally self-limiting acute respiratory tract infections (RTIs), such as sore throat and the common cold, is a global problem an important factor for increasing levels of resistance. A new group international experts—the Global Respiratory Infection Partnership (GRIP)—is committed to addressing this issue, with interface between primary care practitioners their patients core focus. To combat overuse antibiotics community, facilitate change from prescribing...
The alarming emergence and spread of antimicrobial resistance among common bacteria threatens the effectiveness therapy for many infections. Surveillance is essential to identify major problems guide adequate control measures. Several surveillance programs have been implemented in North America Europe last decade; however, very few assessed Latin American countries. SENTRY Antimicrobial Program was initiated 1997 represents most comprehensive program place at present time worldwide. collects...
Central venous catheterization is a common technique to establish rapid and temporary access for hemodialysis. However, it known risk factor Staphylococcus aureus infection bacteremia. Mupirocin topical antibiotic with high in vitro anti-staphylococcal activity. A randomized prospective trial was conducted assess the effectiveness of mupirocin ointment prevention skin catheter colonization, episodes bacteremia 136 end-stage renal disease patients. Of these, 67 received disinfection at...
We report the antimicrobial susceptibility patterns of most frequently isolated Gram-positive bacteria in Brazilian hospitals participating SENTRY Antimicrobial Surveillance Program. The strains were consecutively collected (one per patient) between January 2005 and September 2008 tested by reference broth microdilution methods at JMI Laboratories (North Liberty, Iowa, USA). A total 3,907 cocci analyzed. organisms from bloodstream infections Staphylococcus aureus (2,218 strains; 20.2%...
Emerging resistance phenotypes and antimicrobial rates among pathogens recovered from community-acquired urinary tract infections (CA-UTI) is an increasing problem in specific regions, limiting therapeutic options. As part of the SENTRY Antimicrobial Surveillance Program, a total 611 isolates were collected 2003 patients with CA-UTI presenting at Latin American medical centers. Each strain was tested central laboratory using Clinical Laboratory Standard Institute (CLSI) broth microdilution...
Purpose.: To develop and apply real-time PCR protocols to the detection classification of Gram status bacterial pathogens in aqueous vitreous humor collected from clinically suspected intraocular infections Methods.: The analytical specificity two assays, SYBR Green 16S rDNA–Based Universal (SGRU-PCR), a Multiplex Gram-Specific TaqMan–Based (MGST-PCR), was determined with 31 important pathogens, including 20 Gram-positive 11 Gram-negative. Analytical sensitivity 10-fold dilution...
Nosocomial bloodstream infections (nBSIs) are an important cause of morbidity and mortality the most frequent type nosocomial infection in pediatric patients.We identified predominant pathogens antimicrobial susceptibilities isolates patients (≤16 years age) Brazilian Prospective Surveillance for nBSIs at 16 hospitals from 12 June 2007 to 31 March 2010 (Br SCOPE project).In our study a total 2,563 cases nBSI were reported by participating Br project. Among these, 342 clinically significant...
We report the microbiological characterization of four New Delhi metallo-β-lactamase-1 (blaNDM-1)-producing Enterobacteriaceae isolated in Rio de Janeiro, Brazil. blaNDM-1 was located on a conjugative plasmid and associated with Klebsiella pneumoniae carbapenemase-2 (blaKPC-2) or aminoglycoside-resistance methylase (armA), 16S rRNA not previously reported Brazil, two distinct strains Enterobacter cloacae. Our results suggested that introduction Brazil has been accompanied by rapid spread,...
Bacterial bloodstream infections (BSI) are a common threat among patients with haematological malignancies (HM) and hematopoietic stem cell transplant recipients (HSCT). The purpose of this research was to describe clinical microbiological aspects BSI caused by carbapenem-resistant Klebsiella pneumoniae (CRKp) assess risk factors associated 30-day mortality in 10-year cohort patients. A total 65 CRKp-BSI episodes occurring HM HSCT between January 2010 December 2019 were retrospectively...
Staphylococcus aureus is the most common cause of peritonitis in patients undergoing peritoneal dialysis Brazil. Using restriction endonuclease analysis plasmid DNA, we investigated importance chronic carriage S. development on continuous ambulatory at Division Nephrology, Escola Paulista de Medicina, Sao Paulo, A total 117 isolates (30 patients) were available for typing, including 51 (22 from nares, 58 (27 pericatheter skin, and 8 (6 fluid, with peritonitis. Restriction subtyping showed...
Population antimicrobial use may influence resistance emergence. Resistance is an ecological phenomenon due to potential transmissibility. We investigated spatial and temporal patterns of ciprofloxacin (CIP) population consumption related E. coli emergence dissemination in a major Brazilian city. A total 4,372 urinary tract infection cases, with 723 CIP resistant, were identified 2002 from two outpatient centres. Cases address geocoded digital map. Raw data was transformed into usage density...
Abstract Background The incidence of bloodstream infection ( BSI ) varies according to the transplanted organ. Mortality can be as high 24%, with a significant impact on graft survival. Transplantation is risk factor for multidrug‐resistant MDR organisms, but comparison non‐transplanted population in single large cohort has not been described. Methods This prospective nationwide study (16 centers) reporting data 2364 monomicrobial nosocomial s, comparing 83 episodes solid organ transplant...