A5081988288- Ocular Infections and Treatments
- Neonatal and Maternal Infections
- Streptococcal Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Tuberculosis Research and Epidemiology
- Ocular Surface and Contact Lens
- Antibiotic Resistance in Bacteria
- Mycobacterium research and diagnosis
- Antimicrobial Resistance in Staphylococcus
- Maternal and Neonatal Healthcare
- Corneal surgery and disorders
- Infectious Diseases and Tuberculosis
- Infective Endocarditis Diagnosis and Management
- Reproductive tract infections research
- COVID-19 Clinical Research Studies
- Tracheal and airway disorders
- Cystic Fibrosis Research Advances
- Corneal Surgery and Treatments
- Long-Term Effects of COVID-19
- Pneumonia and Respiratory Infections
- Infections and bacterial resistance
- SARS-CoV-2 and COVID-19 Research
- Escherichia coli research studies
- Vibrio bacteria research studies
- Ocular Diseases and Behçet’s Syndrome
Universidade Federal de São Paulo
2013-2023
Hospital São Paulo
2021
Fundação de Apoio à Universidade Federal de São Paulo
2019
Tampa General Hospital
2016-2018
Universidade Estadual Paulista (Unesp)
2010
We report the microbiological characterization of four New Delhi metallo-β-lactamase-1 (blaNDM-1)-producing Enterobacteriaceae isolated in Rio de Janeiro, Brazil. blaNDM-1 was located on a conjugative plasmid and associated with Klebsiella pneumoniae carbapenemase-2 (blaKPC-2) or aminoglycoside-resistance methylase (armA), 16S rRNA not previously reported Brazil, two distinct strains Enterobacter cloacae. Our results suggested that introduction Brazil has been accompanied by rapid spread,...
ABSTRACT A multiplex real-time PCR was validated on the BD Max open system to detect different Mycobacterium tuberculosis complex, avium and spp. directly from clinical samples. The results were compared those with traditional cultures. assay found be a specific sensitive method for rapid detection of mycobacteria specimens.
ABSTRACT Group B Streptococcus detection directly from Copan ESwab collected samples, using the BD Max GBS assay, was evaluated on receipt in laboratory and after 24 h at room temperature. Results were compared to those Lim broth enrichment PCR culture. No significant difference observed between PCRs.
Infections are the major cause of morbidity and mortality in children with cancer. Gaining a favorable prognosis for these patients depends on selecting appropriate therapy, which turn rapid accurate microbiological diagnosis. This study employed real-time PCR (qPCR) to identify main pathogens causing bloodstream infection (BSI) treated at Pediatric Oncology Institute IOP-GRAACC-UNIFESP-Brazil. Antimicrobial resistance genes were also investigated using this methodology.A total 248 samples...
Abstract Background Early identification of pathogens and antimicrobial resistance in bloodstream infections (BSIs) decreases morbidity mortality, particularly immunocompromised patients. The aim the present study was to compare real-time polymerase chain reaction (PCR) with commercial kits for detection 17 from blood culture (BC) 10 genes. Methods A total 160 BCs were taken bone marrow transplant patients screened Gram-specific probes by multiplex PCR genus-specific sequences using TaqMan...
Vaccines in development against Group B Streptococcus (GBS) should contain the most prevalent capsular genotypes screened target population. In low- and middle-income countries epidemiological data on GBS carriage among pregnant women, a prerequisite condition for neonatal sepsis, is needed to inform vaccine strategies. To investigate prevalence of different that colonizes at-risk women private maternity hospital São Paulo, Brazil. strains isolated routine procedures from 2014 2018 were...
CONTEXT AND OBJECTIVE: Maternal Streptococcus agalactiae colonization and early-onset neonatal sepsis have aroused interest in the worldwide literature. Streptococcal disease is associated with significant morbidity mortality perinatal period, especially among premature neonates. The aim of this study was to assess prevalence maternal streptococcal by using combined swab cultures, compared collection from a single site. DESIGN SETTING: Cross-sectional at Faculdade de Medicina Botucatu,...
While primarily Gram-positive bacteria cause bacterial eye infections, several Gram-negative species also pose health risks. Currently, few studies have tried to understand the pathogenic mechanisms involved in E. coli infections. Therefore, this study aimed establish potential of strains isolated from Twenty-two between 2005 and 2019 patients with keratitis or conjunctivitis were included submitted traditional polymerase chain reactions (PCR) define their virulence profile, phylogeny,...
The aim of this study was to analyze patient data and the laboratory results corneal samples collected from patients followed at Ophthalmology Department, Hospital São Paulo, Brazil over a 30-year period, correlate analize with contact lens wearing. Corneal diagnosed clinically microbial keratitis between January 1987 December 2016 were included in study. Cultures that positive for bacteria, fungi, Acanthamoeba spp. analyzed retrospectively. To ascertain if number contact-lens-associated (as...
The aim of this study was to develop a multiplex PCR test detect Achromobacter xylosoxidans (AX), Burkholderia cepacia (BC), Pseudomonas aeruginosa (PSA) and Stenotrophomonas maltophilia (SM) directly from CF patient's respiratory samples using the open mode BD MAX™ System. A total 402 were evaluated by culture PCR. Specific sets primers probes for each target designed in-house. Out tested, 196 identified as negative 206 positive AX, PSA, BC SM. Among samples, detected 21/27 4/5 BC, 138/140...
Purpose: The aims of this study were to characterize alpha-hemolytic streptococci among isolates from cases infectious endophthalmitis and keratitis determine their distributions. Methods: sample included 27 35 nonduplicated recovered patients with (2002-2013) (2008-2013), respectively. Isolates identified by the optochin susceptibility bile solubility tests, using a biochemical identification system. minimum inhibitory concentration was determined broth microdilution method. Molecular...
Purpose: In vitro evaluation of rose bengal (RB) photoactivated by our custom-built green light-emitting diode (LED) source for the growth inhibition bacterial strains and rapidly growing mycobacterial (RGM) isolates in infectious keratitis. Methods: Six corneal clinical bacteria were included this study: two Gram-positive (methicillin-resistant Staphylococcus aureus [MRSA] epidermidis), Gram-negative (Pseudomonas aeruginosa Serratia marcescens), RGM (Mycobacterium chelonae Mycobacterium...