A5043308001- CAR-T cell therapy research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Autoimmune and Inflammatory Disorders Research
- Immunotherapy and Immune Responses
- Cardiac Structural Anomalies and Repair
- Rheumatoid Arthritis Research and Therapies
- Cardiac Valve Diseases and Treatments
- Pregnancy and Medication Impact
- Virus-based gene therapy research
- Immunodeficiency and Autoimmune Disorders
- Epigenetics and DNA Methylation
- Birth, Development, and Health
- Research on Leishmaniasis Studies
- Infective Endocarditis Diagnosis and Management
- Cancer Immunotherapy and Biomarkers
- Prenatal Screening and Diagnostics
- Cardiac tumors and thrombi
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Metabolism and Genetic Disorders
- Peptidase Inhibition and Analysis
- Pericarditis and Cardiac Tamponade
- Reproductive System and Pregnancy
- Estrogen and related hormone effects
Emory University
2024-2025
Emory and Henry College
2024
Glenmark Pharmaceuticals (Switzerland)
2024
Loyola University Chicago
2016-2023
Hospital de Basurto
2012-2021
University of Lausanne
2007-2020
Hospital de Cruces
1995-2020
Ludwig Cancer Research
2020
University of Chicago
2020
University of Houston
2018
Neutrophils are key components of the inflammatory response and as such contribute to killing microorganisms. In addition, recent evidence suggests their involvement in development immune response. The role neutrophils during first weeks post-infection with Leishmania donovani was investigated this study. When L. donovani-infected mice were selectively depleted NIMP-R14 monoclonal antibody, a significant increase parasite numbers observed spleen bone marrow lesser extent liver. Increased...
Adoptive T-cell therapy using chimeric antigen receptor-modified T cells (CAR-T therapy) has shown dramatic efficacy in patients with circulating lymphoma. However, eradication of solid tumors CAR-T not been reported yet to be efficacious. In tumors, stroma destruction, due MHC-restricted cross-presentation tumor antigens cells, may essential. CAR-Ts recognize an MHC-independent manner on cancer but cells. this report, we show how can engineered eradicate large established provision a...
Abstract Polymorphonuclear neutrophils (PMN) are key components of the inflammatory response contributing to development pathogen-specific immune responses. Following infection with Leishmania major, recruited within hours site parasite inoculation. C57BL/6 mice resistant infection, and BALB/c susceptible developing unhealing, lesions. In this report, we investigated expression cell surface integrins, TLRs, secretion immunomodulatory cytokines by PMN both strains mice, in L. major. The was...
Abstract The macrophage is the niche of intracellular pathogen Mycobacterium tuberculosis. Induction apoptosis by CD4+ or CD8+ T cells accompanied reduced bacterial counts, potentially defining a host defense mechanism. We have already established that M. tuberculosis-infected primary human macrophages susceptibility to Fas ligand (FasL)-induced apoptosis. To study mechanisms which tuberculosis prevents apoptotic signaling, we generated cell culture system based on PMA- and...
Inhibitory checkpoint blockade therapy is an immunomodulatory strategy that results in the restoration of T cell functions, and its efficacy depends on recognition tumor cells for destruction. Considering factors at play, one could propose anti-tumor responses will not occur if are immunologically invisible to cells. In this study, we tested a based modulation cancer cell's immunovisibility through HDAC inhibition. model (heterotopic orthotopic) mouse urothelial bladder cancer, demonstrated...
Abstract The placenta is crucial for fetal development, affected by PFAS toxicity, and evidence accumulating that gestational perturb the epigenetic activity of placenta. Gestational exposure can adversely affect offspring, yet individual cumulative impacts on placental epigenome remain underexplored. Here, we conducted an epigenome-wide association study (EWAS) to examine relationships between levels DNA methylation in a cohort mother-infant dyads Arkansas. We measured 17 human tissues...
Adoptive cell therapy with chimeric antigen receptor (CAR) T cells has transformed standard-of-care for selected hematologic malignancies, but relapses are frequent and efficacy against solid tumors remains limited. The tumor microenvironment (TME) plays a key role in progression, both soluble cellular TME components can limit CAR-T function persistence. Targeting factors to enhance anti-tumor responses of engineered through receptors is not yet broadly explored due the unpredictable...
Abstract Neutrophils are recruited to the site of parasite inoculation within a few hours infection with protozoan Leishmania major. In C57BL/6 mice, which resistant infection, neutrophils cleared from s.c. 3 days, whereas they persist for at least 10 days in susceptible BALB/c mice. present study, we investigated role macrophages (MΦ) regulating neutrophil number. Inflammatory cells were by i.p. injection either 2% starch or L. major promastigotes. isolated and cultured presence increasing...
Adoptive T-cell therapy (ATCT) can result in tumor rejection, yet the behavior and fate of introduced T cells remain unclear. We developed a novel bioluminescence mouse model, which enabled highly sensitive detection signals at single-cell level. Transferred preferentially accumulated within antigen-positive tumors, relative to unaffected areas each mouse, remarkably, expanded both lymphopenic P14 mice. This expansion was controlled efficient, as evaluated by imaging (BLI) rejection...
Adoptive T cell therapy (ATT) can achieve regression of large tumors in mice and humans; however, frequently recur. High target peptide-major histocompatibility complex-I (pMHC) affinity receptor (TCR)-pMHC are thought to be critical preventing relapse. Here, we show that targeting two epitopes the same antigen cancer cells via monospecific cells, which have similar pMHC pMHC-TCR affinity, results eradication large, established when apparently subdominant but not dominant epitope. Only...
<h3>Background</h3> The development of memory responses is an evolutionary function the adaptive immune system. We propose that for system to populate compartment with best-suited CD8 T cells it utilizes a process certification or molecular accreditation mediated through Natural Killer Group 2D (NKG2D). This assures filled have demonstrated their ability kill cognate targets two-step cell receptor (TCR) and NKG2D signaling. <h3>Methods</h3> One week after immunization peptide-pulsed...
Abstract Tetrasomy of short arm chromosome 9 constitutes a clinically recognizable chromosomal syndrome. Isochromosome 9p shows strong propensity to tissue‐limited mosaicism. It occurs predominantly in peripheral blood cultures, often at lower frequency or even absent skin, amniotic fluid chorionic villous cell cultures. Tissue‐limited nature mosaicism may render prenatal detection this condition very difficult. Herein, we report two new cases mosaic tetrasomy 9p. Conventional cytogenetics...
Activated T cells expressing endogenous or transduced TCRs are two cell types currently used in clinical adoptive T‐cell therapy. The ability of these to recognize their antigen, expand and traffic the tumor site initial steps necessary for successful In this study, we vivo bioluminescent imaging (BLI) Renilla luciferase (RLuc) evaluate adoptively transferred survive, home . Using method, termed RT‐Rack (Rluc tracking), followed response against tumors Expansion homing were antigen...
Visualizing oncogene/tumor Ag expression by noninvasive imaging is of great interest for understanding processes tumor development and therapy. We established transgenic (Tg) mice conditionally expressing a fusion protein the SV40 large T luciferase (TagLuc) that allows monitoring bioluminescent upon Cre recombinase-mediated activation. Independent Cre-mediated recombination, TagLuc gene was expressed at low levels in different tissues, probably due to leakiness stop cassette. The level...
AbstractInfections are a major cause of illness and death amongst elderly people. Peripheral blood CD8+ T lymphocytes -which play crucial role in host defence against viral infections-, divided subsets based upon the expression several cell activation markers. Since senescence changes peripheral lymphocyte compartment have been described, studies were performed to determine whether aging there variations CD8+CD38+, CD8+CD57+, CD8+HLA-DR+, CD8+CD45RA+ CD8+CD45RO+ subset. A decrease was...
The virulence of an isogenic pair Pseudomonas aeruginosa strains was studied under similar experimental conditions in two animal infection models. time to death significantly longer for the multidrug resistant (MDR) than wild-type strain. transcriptional profiles 84 innate immune response genes lungs competent Balb/C mice were further compared. Significantly weaker expression involved production soluble pattern recognition receptor and complement observed animals infected with MDR Altered...
Abstract The role of natural killer group 2D (NKG2D) in peripheral T cells as a costimulatory receptor is well established. However, its contribution to cell thymic education and functional imprint unknown. Here, we report significant changes development, signaling, transcriptional program, function from mice lacking NKG2D signaling. In C57BL/6 (B6) OT-I mice, found that deficiency results Vβ chain usage stagnation the double-positive stage development. We expression CD5 CD45 thymocytes...