At present, great interest is caused with evaluation of new markers in blood circulation for the estimation a tissue oxidative metabolism disturbance due to presence secondary mitochondrial dysfunction patients coronary heart disease. Сoronary disease generally accompanied decline respiration and represents root cause metabolic abnormalities tissues. To gain insight into rate tissues there were proposed humanin as marker. The content compare other energy 59 was studied. In examined patients,...

The role of the kidneys in metabolism and homeostasis sulfur-containing amino acids is great, so levels methionine (Met), total homocysteine (tHcy) their ratios can be diagnostic value chronic kidney disease (CKD), a course arterial hypertension (AH). aim study was to evaluate Met/tHcy ratio hypertensive patients with CKD. We used blood plasma 76 aged 40-75 years AH excretory dysfunction kidneys; subgroups: 1 - proteinuria (n=37); 2 without glomerular filtration rate (GFR) < 90 ml/min/1.73...

A large number of studies have established a link between the progression arterial hypertension (AH) and folic acid (FA) deficiency. Due to deficiency FA, an increase in level total homocysteine (tHcy) is observed. The cut-off point for (tHcy), which not associated with toxic effect Hcy itself, indicating FA deficient state, has been clearly defined date. purpose this work was assess cutoff value tHcy applied assesment functional hypertensive patients, including group chronic kidney disease...

Ascending thoracic aortic aneurysm (aTAA) is a heterogeneous group of disorders that involve impaired endothelial function. The nitric oxide (NO) synthase inhibitor asymmetric dimethylarginine (ADMA) serves as an dysfunction marker. Thus, we investigated ADMA levels in patients with aTAA.Eighty-six aTAA and 18 healthy individuals were enrolled. All underwent echocardiography. Plasma measured using high-performance liquid chromatography.ADMA higher than control (p = 0.034). According to the...

The level of peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC1a) in human blood plasma was investigated. Samples healthy individuals (n=34) and patients with cardiovascular diseases (n=110), including aortic aneurysm (n=69), stenosis (n=25) without pathologies were analyzed. In the PGC1a concentration higher than that persons, tended to decrease age. Elevated concentrations lactic acid, total homocysteine asymmetric dimethylarginine suggested a parallel development...

The level of homoarginine (hArg) in terms prognostic significance may exceed the natriuretic peptides and other well-known markers according to latest data about progression cardiovascular diseases. lack on association hArg levels with metabolites makes it difficult understand its role pathogenesis Relationships amino acids, including methionine (Met) total homocysteine (tHcy), their ratio patients ischemic heart disease were evaluated. study included 74 coronary (57 men 17 women) aged 62 -...

Objective of the present study was to estimate concentrations methylated derivatives L‑lysine and L‑arginine in patients with blood circulation disorders. Design methods. We examined plasma samples 151 cardiovascular diseases, including 86 aortic aneurysm 47 stenosis, as well normal subjects divided two age groups. The trimethyl-L‑lysine (TML), symmetric dimethyl-L‑arginine (SDMA) asymmetric (ADMA) were determined by liquid chromatography after solid phase extraction. Results. Decreased TML...

BACKGROUND. Evaluation the level of citrulline (Cit) in blood allows us to determine metabolic disorders proximal tubules kidneys. Previously, we showed that impaired function kidneys hypertensive patients (AH) is expressed by an increase Cit. THE AIM was diagnostically significant Cutoff value or exceeding Cit levels separately for men and women with hypertension. PATIENTS AND METHODS. We examined 115 hyperten sive estimated glomerular filtration rate ≥ 45 ml/min/1.73 m 2 , included 44 71...

According to current data, a low level of folic acid (FA) contributes the progression arterial hypertension (AH), affecting metabolism cells that are involved in vascular tone regulation, such as hypothalamic astrocytes nervous tissue. It is also known FA tissue and cerebrospinal fluid 2-3 times higher than plasma. There another metabolic marker cardiovascular diseases, plasma homoarginine (hArg). The decrease hArg diagnostic sign. In our study, we established patients with AH (n = 60), was...

Elevated levels of homocysteine (Hcy) in tissues cause cytotoxic effects. Increase the overall conсentration various Hcy chemical forms above 12 μM is called hyperhomocysteinemia. The term «total homocysteine» plasma (tHcy) sum concentrations aminothiol reduced (-SH) and oxidized (-SS-) forms. It was suggested that S- and, partially, N-homocysteinylation proteins plays a major role toxic effects Hcy. rate tHcy not closely related to pathological process with remodeling vascular wall entirely...

In the analysis of plasma amino acid profile in a group patients with left ventricular outflow tract pathology (n = 151) increased levels serine, alanine, arginine, and lysine has been found. These metabolic shifts can be linked development circulatory deficiency mitochondrial dysfunction. The differentiation reference values intervals helps assessment individual profiles.

The article deals with study concerning content of cytochrome C (CytC) and protein PGC1α (1alpha-co-activator gammareceptor, activating proliferation peroxisomes) in blood patients pathology left ventricular outflow tract conjointly low-molecular metabolites characterizing dysfunction mitochondria endothelium. samples plasma were analyzed taking from healthy persons (n=34), aortic aneurysm (n=69), stenosis (n=25) without aorta (n=16). In the group disturbed circulation increasing level...

Hypertension (HTN) is often accompanied by folic acid (FA) deficiency and hyperhomocysteinemia (HHcy). Reduced glutathione (GSH) dependent enzymes determine the state of cellular antioxidant redox systems in cardiovascular pathology. The aim our work to assess relationship between status FA presence HHcy with metabolism erythrocyte HTN. Design methods. In blood plasma samples from 43 HTN patients admitted clinic Pavlov University, concentration total homocysteine (oHcy) was determined. We...

Background. High blood concentration of D-dimer is a reliable marker activation intravascular coagulation, but does not provide unambiguous information about the functional potential ibrinolytic system. Objective. To determine contribution hyperhomocysteinemia (HHcy) in ibrinolysis disturbances patients with activated coagulation. Design and methods. Plasma samples 141 coagulation various etiologies [82 women (58,2 %) 59 men (41,8 %), mean age 53,2 ± 7,8 years] level &gt; 500 ng/ml were...

Low plasma L-homoarginine (hArg) concentration is an independent predictor of adverse cardiovascular outcomes and overall mortality, as well the progression chronic kidney disease (CKD). The enzyme L-arginine:glycinamidinotransferase (AGAT, EC 2.1.4.1) acts in mitochondrial membrane renal tubular epithelium, forming precursor creatine, guanidinoacetic acid, additionnaly by-product hArg. As it was shown recently, there a decreased level hArg late stages CKD, however, early CKD remained...

Objective . To assess homoarginine (hArg) level in patients with ascending aortic aneurysm and stenosis. Design methods The study included 26 19 comparison group consisted of 30 healthy donors. Plasma levels hArg, asymmetric dimethylarginine (ADMA), the parameters mitochondrial dysfunction (pyruvic acid, PGC-1a protein, lactic cytochrome C) were determined. Results Patients stenosis demonstrated a decrease serum hArg (p = 0,002), most pronounced aneurysm, an increase ADMA markers...

The aim of the work was to identify relationship vasomotor and angiogenic factors endothelium, as well state antioxidant system in rheumatoid arthritis (RA). Materials methods included 50 patients with RA 42 healthy people appropriate age gender, who analyzed main clinical indicators. In erythrocytes, activity superoxide dismutase (SOD), glutathione peroxidase (GPO), reductase (GR) concentration reduced (GSH) were investigated. higher nitrogen oxides (NOx), endothelin-1 (ET-1) vascular...

The homoarginine is proposed as a prognostic metabolic criterion of danger stroke and myocardium infarction evaluated in this capacity number population studies. actual study analyzed content cardio-metabolic marker main amino acids blood plasma 59 patients (46 males 13 females, aged 61,4 ± 6,2 years) wit6h ischemic heart disease send to planned operation aortocoronary bypass surgery also 30 healthy individuals 50,4 9,2 years. concentration was detected using high-performance liquid...

It has been found out that methylation of nucleic acids, proteins and low-molecular substrates is necessary to renew cellular structures, their restoration cytoprotection. Methionine plays the most important role in this process but whether its metabolism changes during anesthesia how different anesthetics affect it, not yet studied. The objective : study methionine cardiopulmonary bypass when direct myocardial revascularization performed impact used (propofol, desflurane, sevoflurane) on...