A5078586691- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Retinal Development and Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Neuroscience and Neural Engineering
- Retinal Diseases and Treatments
- Neuroscience and Neuropharmacology Research
- Retinal Imaging and Analysis
- Glaucoma and retinal disorders
- Animal Genetics and Reproduction
- Innovation and Socioeconomic Development
- Genetics, Aging, and Longevity in Model Organisms
- Nicotinic Acetylcholine Receptors Study
First Affiliated Hospital of Jinan University
2018-2023
The University of Melbourne
2018-2020
Centre for Eye Research Australia
2018-2020
Jinan University
2018-2019
Objective This study aims to develop and evaluate a non-imaging clinical data-based nomogram for predicting the risk of vision-threatening diabetic retinopathy (VTDR) in diabetes mellitus type 2 (T2DM) patients. Methods Based on baseline data Guangdong Shaoguan Diabetes Cohort Study conducted by Zhongshan Ophthalmic Center (ZOC) 2019, 2294 complete T2DM patients were randomly divided into training set (n=1605) testing (n=689). Independent factors selected through univariate multivariate...
Gain-of-function studies often require the tedious cloning of transgene cDNA into vectors for overexpression beyond physiological expression levels. The rapid development CRISPR/Cas technology presents promising opportunities to address these issues. Here, we report a simple, cloning-free method induce gene at an endogenous locus using CRISPR/Cas9 activators. Our strategy utilizes synthesized sgRNA cassettes direct nuclease-null Cas9 complex fused with transcriptional activators (VP64, p65,...
Summary The retina is a highly specialized neural tissue that senses light and initiates image processing. Although the functional organisation of specific cells within has been well-studied, molecular profile many cell types remains unclear in humans. To comprehensively human retina, we performed single RNA-sequencing on 20,009 obtained post-mortem from three donors compiled reference transcriptome atlas. Using unsupervised clustering analysis, identified 18 transcriptionally distinct...
Abstract Gain-of-function studies often require the tedious cloning of transgene cDNA into vectors for overexpression beyond physiological expression levels. The rapid development CRISPR/Cas technology presents promising opportunities to address these issues. Here we report a simple, cloning-free method induce gene at endogenous locus using CRISPR/Cas9 activators. Our strategy utilises synthesized sgRNA cassettes direct nuclease-null Cas9 complex fused with transcriptional activators (VP64,...
Current advances in cellular reprogramming technology has demonstrated that the identity of a cell can be converted by use master transcription factors to reprogram transcriptome.Notably, this allows us convert somatic cells into induced pluripotent stem (iPSCs), providing feasible method generate patient-specific cells.This was firstly discovered Shinya Yamanaka's group 2006.The initial iPSCs were formed induction dedifferentiation mouse fibroblasts using factors: Oct4, Sox2, Klf4 and...