A5103112773- Neuroscience and Neuropharmacology Research
- Anesthesia and Neurotoxicity Research
- Mitochondrial Function and Pathology
- Glaucoma and retinal disorders
- Corneal Surgery and Treatments
- Cardiac Ischemia and Reperfusion
- Intramuscular injections and effects
- RNA Interference and Gene Delivery
- Cerebral Venous Sinus Thrombosis
- Endoplasmic Reticulum Stress and Disease
- Corneal surgery and disorders
- Pluripotent Stem Cells Research
- Retinal Diseases and Treatments
- Ocular Diseases and Behçet’s Syndrome
- Pediatric health and respiratory diseases
- Vaccine Coverage and Hesitancy
- Ophthalmology and Visual Impairment Studies
- Cancer-related Molecular Pathways
- Ocular Oncology and Treatments
- Healthcare cost, quality, practices
- Receptor Mechanisms and Signaling
- Calpain Protease Function and Regulation
- ATP Synthase and ATPases Research
- Cerebrovascular and genetic disorders
- Smoking Behavior and Cessation
Oslo University Hospital
2011-2024
University of Oslo
2011-2024
Institutt for Eksperimentell Medisinsk Forskning
2023
United States Department of Homeland Security
2016
Institute for Medical Research
2004
The growth and recurrence of several cancers appear to be driven by a population cancer stem cells (CSCs). Glioblastoma, the most common primary brain tumor, is invariably fatal, with median survival approximately 1 year. Although experimental data have suggested importance CSCs, few exist regarding potential relevance these in clinical setting.We here present first seven patients treated dendritic cell (DC)-based vaccine targeting CSCs solid tumor. Brain tumor biopsies were dissociated into...
Background: The mitochondrial membrane potential (ΔΨ m ) controls the generation of adenosine triphosphate (ATP) and reactive oxygen species, sequesteration intracellular Ca 2+ [Ca ] i . Clinical concentrations sevoflurane affect ΔΨ in neural mitochondria, but mechanisms remain elusive. aim present study was to compare effect isoflurane on rat pre‐synaptic terminals (synaptosomes), investigate whether these agents by inhibiting respiratory chain. Methods: Synaptosomes were loaded with...
The mitochondrial membrane potential drives the main functions of mitochondria. Sevoflurane depolarizes neural There is still, however, limited information concerning effect anaesthetics on mitochondria in humans. sevoflurane and propofol intracellular Ca(2+) concentration [Ca(2+)](i) (DeltaPsi(m)) was therefore compared rat human synaptosomes, changes were related to interventions electron transport chain.Synaptosomes from cerebral cortex loaded with fluorescent probes fura-2 ([Ca(2+)](i))...
Background: Volatile anaesthetics protect the heart from ischaemic injury by activating mitochondrial signalling pathways. The aim of this study was to test whether sevoflurane, which is increasingly used in neuroanaesthesia, affects function central nervous system altering membrane potential (ΔΨ m ). Methods: In order correlate free cytosolic Ca 2+ ([Ca ] i ) and ΔΨ , rat neural presynaptic terminals (synaptosomes) were loaded with fluorescent probes fura‐2 JC‐1. During sevoflurane...
Protein kinase C (PKC) is an important enzyme involved in the regulation of neurotransmission and might also be mediation ischemic neuronal death. PKC has been implicated as a target volatile anesthetics well anesthetic protection against ischemia. The present study tested effect isoflurane sevoflurane, both used neuroanesthetic practice, on presynaptic free cytosolic Ca2+ ([Ca2+](i)) activity. To measure [Ca2+](i) activation simultaneously, rat synaptosomes, mostly containing terminals,...
The mitochondrial membrane potential (DeltaPsi(m)) drives the three fundamental functions of mitochondria, namely adenosine triphosphate (ATP) generation, Ca(2+) uptake/storage, and generation/detoxification ROS. Isoflurane depolarizes neural mitochondria. sensitivity for general anesthetics increases with age, but mechanism this age-related is still unknown. We compared effect isoflurane on [Ca(2+)](i) DeltaPsi(m) in isolated pre-synaptic terminals (synaptosomes) from neonatal, adolescent,...
In piglets we tested the applicability of digital video microscopy and diffuse reflectance spectroscopy for non-invasive assessments limbal bulbar conjunctival microcirculation. A priori postulated that metabolic rate is higher in as compared to conjunctiva, this difference reflected microvascular structure or function between two locations. Two study sites, Oslo University Hospital (OUH), Norway Cleveland Clinic (CC), USA, used same techniques record microcirculation sleeping piglets....
Non-pulsatile cardiopulmonary bypass (CPB) may induce microvascular dysregulation. In piglets, we compared ocular surface microcirculation during pulsatile versus continuous flow (CF) bypass.Ocular in small tissue volumes (~0.1 mm3 ) at limbus (high metabolic rate) and bulbar conjunctiva (low was examined a porcine model using computer assisted video microscopy diffuse reflectance spectroscopy, before after 3 6 h of (n = 5 piglets) or CF CPB. Functional capillary density, velocity oxygen...
Aims To assess the feasibility of a study protocol for randomised controlled trial zoledronic acid (ZA) as adjuvant therapy neovascular age-related macular degeneration (nAMD). Methods In this 1-year, randomised, double-blinded, placebo-controlled pilot study, nAMD patients were allocated 1:1 to receive intravenous ZA 5 mg or placebo at baseline and after 6 months in addition intravitreal anti-vascular endothelial growth factor (anti-VEGF) following treat-and-extend regimen. Bevacizumab was...
Background: A possible benefit of optical coherence tomography (OCT) in the approach to tumors involving optic chiasm may be ability foresee visual deterioration. This study investigated value OCT watchful waiting for compressive neuropathy as primary management suprasellar masses. Methods: The research was conducted a 2-year observational patient cohort with conservatively managed mass lesions on MRI. Threshold perimetry and macular were performed at baseline each follow-up examination....
Purpose To examine correlation of DNA methylation patterns human uveal melanoma with survival. Methods FFPE samples from 24 UM patients undergoing enucleation the eye in period 1976–1989 were included. Inclusion was based on histopathological data, data Norwegian Cancer Registry and Cause Death Registry. isolated further bisulphite converted restored according to Illuminas recommendations. Bisulphite then run The Infinium HumanMethylation450 BeadChip assay (HM‐450K). Bioinformatics analyses...
BACKGROUND Neurological disorders can present with a vast array of visual disturbances. The constellation symptoms and findings in this patient prompted workup for unusual causes both stroke neurodegenerative disorder. CASE PRESENTATION A woman her sixties presented disturbances, followed by weakness right arm aphasia three days later. Her close acquaintances had suspected progressive cognitive decline during the previous year. CT MRI showed an occluded left posterior cerebral artery...