A5005479598- Corneal Surgery and Treatments
- Epigenetics and DNA Methylation
- Corneal surgery and disorders
- Pluripotent Stem Cells Research
- Mesenchymal stem cell research
- Ocular Surface and Contact Lens
- Ocular Oncology and Treatments
- Tissue Engineering and Regenerative Medicine
- Ocular Disorders and Treatments
- Genomics and Chromatin Dynamics
- Hedgehog Signaling Pathway Studies
- Cancer-related gene regulation
- Gene Regulatory Network Analysis
- CRISPR and Genetic Engineering
- Telomeres, Telomerase, and Senescence
- Intraocular Surgery and Lenses
- RNA modifications and cancer
- Angiogenesis and VEGF in Cancer
- Circadian rhythm and melatonin
- Bladder and Urothelial Cancer Treatments
- Muscle Physiology and Disorders
- Retinal Development and Disorders
- Folate and B Vitamins Research
- Wound Healing and Treatments
- Ubiquitin and proteasome pathways
University of Oslo
2007-2024
Oslo University Hospital
2014-2024
Functional reprogramming of a differentiated cell toward pluripotency may have long-term applications in regenerative medicine. We report the induction dedifferentiation, associated with genomewide programming gene expression and epigenetic an embryonic gene, epithelial 293T cells treated extract undifferentiated human NCCIT carcinoma cells. exposed for 1 h to cells, but not or Jurkat T-cells, form defined colonies that are maintained at least 23 passages culture. Microarray quantitative...
Abstract The potential use of human mesenchymal stem cells for therapeutic applications implies large scale in vitro culture, increasing the probability genetic instability and transformation. We examine here incidence unbalanced balanced chromosome rearrangements polyclonal single cell‐derived cultures adipose to senescence. G‐banding karyotyping shows a normal karyotype. In addition, high‐resolution microarray‐based comparative genomic hybridization analyses relative uncultured from same...
Mesenchymal stem cells from adipose tissue can differentiate into mesodermal lineages. Differentiation potential, however, varies between clones of (ASCs), raising the hypothesis that epigenetic differences account for this variability. We report here a bisulfite sequencing analysis CpG methylation adipogenic (leptin [LEP], peroxisome proliferator-activated receptor gamma 2 [PPARG2], fatty acid-binding protein 4 [FABP4], and lipoprotein lipase [LPL]) promoters nonadipogenic (myogenin [MYOG],...
Abstract Background Potential therapeutic use of mesenchymal stem cells (MSCs) is likely to require large-scale in vitro expansion the before transplantation. MSCs from adipose tissue can be cultured extensively until senescence. However, little known on differentiation potential (ASCs) upon extended culture and associated epigenetic alterations. We examined adipogenic clones human ASCs early passage senescence, determined whether senescence was with changes promoter DNA methylation. Results...
ABSTRACT Inhibition of sclerostin, a glycoprotein secreted by osteocytes, offers new therapeutic paradigm for treatment osteoporosis (OP) through its critical role as Wnt/catenin signaling regulator. This study describes the epigenetic regulation SOST expression in bone biopsies postmenopausal women. We correlated serum sclerostin to mineral density (BMD), fractures, and remodeling parameters, related these findings genetic disease mechanisms. Serum biomarkers were measured two groups:...
Long-term culture of mesenchymal stem cells leads to a loss differentiation capacity, the molecular mechanism which remains not understood. We show here that expansion adipose (ASCs) late passage (replicative senescence) is associated with promoter-specific and global changes in epigenetic histone modifications. In undifferentiated ASCs, inactive adipogenic myogenic promoters are enriched repressive combination trimethylated H3K4 (H3K4m3) H3K27m3 absence H3K9m3, heterochromatin mark....
Abstract In vivo endothelial commitment of adipose stem cells (ASCs) has scarcely been reported, and controversy remains on the contribution ASCs to vascularization. We address epigenetic lineage. report a bisulfite sequencing analysis CpG methylation in promoters two endothelial-cell-specific genes, CD31 CD144, freshly isolated cultures before after induction differentiation. contrast tissue-derived (CD31+) cells, display heavily methylated promoter mosaically CD144 despite basal...
Adipose-tissue-derived stem cells (ASCs) have received considerable attention due to their easy access, expansion potential, and differentiation capacity. ASCs are believed the potential differentiate into neurons. However, mechanisms by which this may occur remain largely unknown. Here, we show that culturing under active proliferation conditions greatly improves propensity toward osteogenic, adipogenic, neurogenic lineages. Neurogenic-induced express early genes as well markers of mature...
Long-term cultures of cornea limbal epithelial stem cells (LESCs) were developed and characterized for future tissue engineering clinical applications. The explants cultivated expanded more than 3 months in medium containing serum as the only growth supplement without use scaffolds. Viable 3D cell outgrowth from was observed within 4 weeks cultivation. outgrowing examined by immunofluorescent staining putative markers stemness (ABCG2, CK15, CK19 Vimentin), proliferation (p63α, Ki-67), basal...
Uveal melanoma (UM) is an aggressive malignancy, in which nearly 50% of the patients die from metastatic disease. Aberrant DNA methylation recognized as important epigenomic event carcinogenesis. Formalin-fixed paraffin-embedded (FFPE) samples represent a valuable source tumor tissue, and recent technology has enabled use these genome-wide analyses. Our aim was to investigate differential relation histopathological classification survival data. In addition we sought identify aberrant genes...
Stem cells have the ability to self-renew, and give rise one or more differentiated cell types. Embryonic stem can differentiate into all types of body unlimited self-renewal capacity. Somatic are found in many adult tissues. They an extensive but finite lifespan a restricted range Increasing evidence indicates that multilineage differentiation is defined by potential for expression developmentally regulated transcription factors lineage specification genes. Gene expression, as emphasized...
Human limbal epithelial stem cells (hLESCs) continuously replenish lost or damaged human corneal cells. The percentage of stem/progenitor in autologous ex vivo expanded tissue is essential for the long-term success transplantation patients with cell deficiency. However, molecular processes governing stemness and differentiation state hLESCs remain uncertain. Therefore, we sought to explore impact canonical Wnt/β-catenin signaling activation on by treating hLESC cultures GSK-3 inhibitor...
We aimed to investigate whether a novel technique of human amniotic membrane (HAM) preparation that mimics the crypts in limbus enhances number progenitor cells cultured ex vivo. The HAMs were sutured on polyester (1) standardly, obtain flat HAM surface, or (2) loosely, achieving radial folding mimic limbus. Immunohistochemistry was used demonstrate higher positive for markers p63α (37.56 ± 3.34% vs. 62.53 3.32%, p = 0.01) and SOX9 (35.53 0.96% 43.23 2.32%, 0.04), proliferation marker Ki-67...
Purpose/Aim: The adult human retina has limited regenerative potential, and severe injury will result in permanent damage. Lower vertebrates handle retinal by activating neural stem cells (NSCs) the ciliary marginal zone (CMZ). Müller glia-like expressing markers of NSCs are also present peripheral (PR) eye, leading to hypothesis that a CMZ-like might exists humans. In order shed further light on this we investigated vitro differentiation potential proliferative isolated from PR towards...
Abstract Late spontaneous in-the-bag intraocular lens (IOL) dislocation is a complication presenting 6 months or later after cataract surgery. We aimed to characterize the cells in capsules (LCs) of 18 patients with late IOL dislocation. Patients' average age was 82.6 ± 1.5 years (range 72–98), and most them had pseudoexfoliation syndrome (PEX). Cells from LCs were positive for myofibroblast (αSMA), proliferation (Ki-67, PCNA), early development/lens progenitor (SOX2, PAX6), chemokine...
Aims/Purpose: Identify the distribution of most commonly used markers for stemness, quiescence, proliferation, and differentiation in limbal compartments such as human epithelial crypts, posterior anterior limbus, well central cornea . Methods: Corneal‐limbal biopsies from cadaver donors ( n = 3) embedded paraffin were proceeded immunohistochemistry fluorescence examined stemness/progenitor (p63α, SOX9, ABCG2, N ‐cadherin), proliferation (ki‐67), quiescence marker (CEBPD), (CX43, KRT3,...
Introduction: This report presents a case of an exceptionally delayed distant recurrence choroidal melanoma, occurring 4 decades after the enucleation affected eye. Case Presentation: In 1977, 29-year-old man underwent for melanoma. At age 68 years, he was diagnosed with advanced prostate cancer. Although metastatic cancer responded to treatment, persistent lung lesion warranted further examination. A biopsy, somewhat surprisingly, confirmed presence melanoma metastasis, enucleation. The...
Multilamellar bodies (MLBs) are concentric cytoplasmic membranes which form through an autophagy-dependent mechanism. In the cornea, presence of MLBs is associated with Schnyder corneal dystrophy (SCD). Ex vivo 3D modelling stroma and SCD can help study pathogenesis resolution disorder.Corneal explants were isolated from cadavers cultivated long-term for more than 3 months to achieve spontaneous outgrowth stroma-derived mesenchymal stem-like cells (CSMSCs). The tissues then examined by...
Purpose To examine the effect of medium and substrate on morphology, expression selected keratins global gene in ex vivo engineered corneo‐limbal grafts. Methods Limbal biopsies retrieved from corneo‐scleral rings cadaveric donors were placed human amniotic membranes or plastic inserts cultured for 3 weeks parallel either a complex (COM) containing serum (HM) as single growth promoting supplement at 37°C 5% CO 2 . Culture was changed every 2–3 days. Grafts examined using light microscopy...