A5058720560- Ocular Surface and Contact Lens
- Corneal Surgery and Treatments
- Cardiac Fibrosis and Remodeling
- Corneal surgery and disorders
- Atrial Fibrillation Management and Outcomes
- Prenatal Substance Exposure Effects
- Organ Transplantation Techniques and Outcomes
- Connective Tissue Growth Factor Research
- Clinical Nutrition and Gastroenterology
- IL-33, ST2, and ILC Pathways
- Cardiac Valve Diseases and Treatments
- Cannabis and Cannabinoid Research
- Legionella and Acanthamoeba research
Charles University
2013-2025
General University Hospital in Prague
2023-2024
Background Atrial fibrillation (AF) is the most common arrhythmia and despite obvious clinical importance remains its pathogenesis only partially explained. A relation between inflammation AF has been suggested by findings of increased inflammatory markers in patients. Objective The goal this study was to characterize morphologically functionally CD45-positive cell populations atrial myocardium patients with as compared sinus rhythm (SR). Methods We examined 46 subjects (19 AF, 27 SR)...
One of the generally recognized factors contributing to initiation and maintenance atrial fibrillation (AF) is structural remodeling myocardium that affects both cardiomyocytes as well interstitium. The goal this study was characterize morphologically functionally interstitium atria in patients with AF or sinus rhythm (SR) who were indicated heart surgery. Patient population consisted 46 subjects (19 long-term persistent AF, 27 SR) undergoing coronary bypass valve Peroperative bioptic...
Atrial fibrosis is considered as the basis in development of long-standing atrial fibrillation (AF). However, advanced heart failure (HF), independent role for AF less clear since HF itself leads to scarring. Our study aimed differentiate patients with from without a population consisting HF. Myocardial samples right and left ventricular wall were obtained during transplantation explanted hearts 21 male Long-standing was present 10 them remaining 11 served sinus rhythm controls....
The aim of this study was to compare concentrations endogenous N-acylethanolamine (NAE) lipid mediators-palmitoylethanolamide (PEA), oleoylethanolamide (OEA), and anandamide (AEA)-in fresh, decontaminated, cryopreserved, freeze-dried amniotic membrane (AM) allografts, thereby determining whether AM's analgesic anti-inflammatory efficiency related NAEs persists during storage. were measured using ultra-high-performance liquid chromatography-tandem mass spectrometry. Indirect fluorescent...
To compare concentrations of endogenous N-acylethanolamines (NAEs) lipid mediators pal-mitoylethanolamide (PEA), oleoylethanolamide (OEA), and anandamide (AEA) in fresh, decon-taminated, cryopreserved freeze-dried amniotic membrane (AM) allografts. Thereby deter-mining whether AM's analgesic anti-inflammatory efficiency related to NAEs persists during storage. The were measured using ultra-high-performance liquid chro-matography-tandem mass spectrometry. Indirect fluorescent...
In this study, we tested a method for long-term storage of oral mucosal epithelial cells (OMECs) so that the could be expanded in vitro after cryopreservation and used treatment bilateral limbal stem cell deficiency. The ability suspended primary OMECs to proliferate was compared OMEC cultures had undergone same process. Both were preserved standard complex medium (COM) with or without cryoprotective agents (CPAs) (glycerol at 5 % 10 dimethyl sulphoxide %). We found cryopreservation, form...
Purpose: To assess the impact of Acanthamoeba keratitis (AK) and amniotic membrane transplantation (AMT) in corneal explants on presence two multifunctional endogenous lectins, i.e. galectins-1 -7.Methods: Ten from AK patients (five with previous AMT five controls without this treatment) seven specimens disease-free control cornea were processed by indirect fluorescent immunohistochemistry.Results: Immunostaining for both galectins was obtained epithelium, stroma endothelial layer all...