This paper describes the use of surface plasmon resonance (SPR) spectroscopy and self-assembled monolayers (SAMs) to determine characteristics functional groups that give surfaces ability resist nonspecific adsorption proteins from solution. Mixed SAMs presenting different were prepared for screening using a synthetic protocol based on reaction organic amines with SAM terminated by interchain carboxylic anhydride groups. Surfaces presented derivatives oligo(sarcosine), N-acetylpiperazine,...

This paper describes the use of surface plasmon resonance spectroscopy and self-assembled monolayers (SAMs) alkanethiols on gold to evaluate ability surfaces terminating in different combinations charged groups resist nonspecific adsorption proteins from aqueous buffer. Mixed SAMs formed a 1:1 combination thiol terminated trimethylammonium group sulfonate adsorbed less than 1% monolayer two with characteristics: fibrinogen lysozyme. Single-component thiols combining positively moiety...

ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXTSurveying for Surfaces that Resist the Adsorption of ProteinsRobert G. Chapman, Emanuele Ostuni, Shuichi Takayama, R. Erik Holmlin, Lin Yan, and George M. WhitesidesView Author Information Department Chemistry Chemical Biology Harvard University, 12 Oxford Street Cambridge, Massachusetts 02138 Cite this: J. Am. Chem. Soc. 2000, 122, 34, 8303–8304Publication Date (Web):August 12, 2000Publication History Received3 March 2000Published online12...

This paper examines the hypothesis that surfaces resistant to protein adsorption should also be adhesion of bacteria (Staphylococcus aureus, Staphylococcus epidermidis) and attachment spreading mammalian cells (bovine capillary endothelial (BCE) cells). The tested were those self-assembled monolayers (SAMs) terminated with derivatives tri(sarcosine) (Sarc), N-acetylpiperazine, permethylated sorbitol, hexamethylphosphoramide, phosphoryl choline, an intramolecular zwitterion...

Intolerance to oxygen is a key limitation in many reactions and particularly controlled radical polymerizations. Here we introduce the use of enzymes such as glucose oxidase (GOx) deoxygenate reversible addition–fragmentation chain transfer polymerizations (Enz-RAFT), facilitating preparation highly polymers vessels open ambient oxygen. Because removal so efficient, very low concentrations GOx initiator can be used, enabling excellent control which demonstrated by pseudoliving polymerization...

A benchtop approach is developed for the synthesis of various polymeric architectures using an aqueous Reversible Addition–Fragmentation chain Transfer (RAFT) photopolymerization technique.

The complexity of polymer-protein interactions makes rational design the best polymer architecture for any given biointerface extremely challenging, and high throughput synthesis screening polymers has emerged as an attractive alternative. A porphyrin-catalysed photoinduced electron/energy transfer-reversible addition-fragmentation chain-transfer (PET-RAFT) polymerisation was adapted to enable complex architectures in dimethyl sulfoxide (DMSO) on low-volume well plates presence air. system...

This paper describes the design and preparation of thin polymeric films that resist adsorption proteins adhesion bacteria to an extent comparable to, or better than, self-assembled monolayers (SAMs) present tri(ethylene glycol) groups. These were prepared by reaction a polyamine, for example, poly(ethylenimine), with SAM presented interchain carboxylic anhydride groups, subsequent conversion amino groups polymer amido on acyl chlorides. Polyamines functionalized acetyl chloride produced...

This paper describes a procedure for preparing mixed self-assembled monolayers (mixed SAMs) on gold that resist the nonspecific adsorption of proteins from solution. method was tested using α-amino derivatives ω-hydroxy- and ω-methoxy-oligo(ethylene glycols): H2N(CH2CH2O)nCH3 H2N(CH2CH2O)nH (n = 3, 6). Mixed SAMs were prepared by allowing these amines to react with SAM presenting interchain carboxylic anhydride groups. The resistance resulting surfaces several proteinscarbonic anhydrase (EC...

Purine nucleosides have been reported to ex- tend the preservation of red cells stored in acid citrate dextrose (ACD) (1-6).This effect was originally attributed production phos- phorylated ribose as an energy substrate within erythrocyte (7-9) through action nu- cleoside phosphorylase (10).This enzyme shown act on inosine, guanosine (11, 12), and adenosine after its conversion inosine by deaminase (3, 13-19).Since preferred over ( 11 ) much less toxic than (2, 3, 16, 18, 20), it selected...

Abstract The synthesis of well‐defined polymers in a low‐volume, combinatorial fashion has long been goal polymer chemistry. Here, we report the preparation wide range highly controlled homo and block co‐polymers by Enz‐RAFT (enzyme‐assisted reversible addition–fragmentation chain transfer) polymerization microtiter plates open atmosphere. addition 1 μ m glucose oxidase (GOx) to water/solvent mixtures enables reactions proceed extremely low volumes (40 μL) radical concentrations. This...

Partial joint repair is a surgical procedure where an artificial material used to replace localised chondral damage. These bearing surfaces must articulate against cartilage, but current materials do not replicate both the biphasic and boundary lubrication mechanisms of cartilage. A research challenge therefore exists provide that mimics In this work polymeric network biomimetic lubricant, poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC), was incorporated into ultra-tough double (DN)...

We investigate a high throughput approach to polymer synthesis by employing photoinduced electron/energy transfer–reversible addition-fragmentation chain transfer (PET-RAFT) polymerization. Polymerization of broad range monomers, including acrylates, methacrylates, acrylamides, and styrenic was achieved directly in multiwell plate 5,10,15,20-tetraphenylporphine zinc (ZnTPP) as photocatalyst under yellow LED light. Various parameters such monomer concentration degree polymerization were...

A rapid and highly sensitive point-of-care (PoC) lateral flow assay for phospholipase A2 (PLA2) is demonstrated in serum through the enzyme-triggered release of a new class biotinylated multiarmed polymers from liposome substrate. Signal enzyme activity generated by adhesion polystreptavidin-coated gold nanoparticle networks to device, which leads appearance red test line due localized surface plasmon resonance effect gold. The use as substrate multivalent linkers link nanoparticles...

Combinatorial and high throughput (HTP) methodologies have long been used by the pharmaceutical industry to accelerate rate of drug discovery. HTP techniques can also be applied in polymer chemistry more efficiently elucidate structure–property relationships, increase speed new material development, rapidly optimize polymerization conditions. Controlled living/radical (CLRP) is widely employed preparation potential materials for bioapplications being suitable a large variety polymeric with...

SUMMARY Hexokinase, aldolase, glyceraldehyde‐ 3 ‐phosphate dehydrogenase, pyruvate kinase and glutamic‐oxalacetic transaminase activities, the rate of glycolysis have been measured in red cells from seven adult males responding to repeated phlebotomy or erythropheresis with iron replacement. The change median age cell populations these men was estimated rates loss erythropoietic These enzyme activities glycolytic were also a patient aplastic anaemia whom transfused aging at known rate....

We review the advantages and drawbacks of various synthetic strategies for conjugating peptides to polymers obtained from reversible-deactivation radical polymerization (also known as living polymerization, controlled or controlled/living polymerization). By using a selection examples, we summarize concept behind divergent convergent syntheses, which lead production linear grafted peptide/polymer conjugates. In second section this review, present an overview near–universal approach...

We have established a new strategy to produce functional organic nanotubes of controlled structure from cyclic peptide/polymer conjugates. The guiding peptide motif was coupled the polymeric chains viacopper-catalyzed azide–alkyne click reaction, yield very well-defined resulting conjugates were then self-assembled into in solution. able control some extent length nanotubes, by modifying polymer chain as well mixing different molecular weights together. In similar fashion we prepare tubes...

Translating controlled/living radical polymerization (CLRP) from batch to the high throughput production of polymer libraries presents several challenges in terms both synthesis and characterization. Although recently there have been significant advances field low volume, CLRP, techniques able simultaneously monitor multiple polymerizations an "online" manner not yet developed. Here, we report our discovery that 5,10,15,20-tetraphenyl-21H,23H-porphine zinc (ZnTPP) is a self-reporting...

Hybrid self‐assembly has become a reliable approach to synthesize soft materials with multiple levels of structural complexity and synergistic functionality. In this work, photoluminescent graphene quantum dots (GQDs, 2–5 nm) are used for the first time as molecule‐like building blocks construct self‐assembled hybrid label‐free biosensors. Ionic disc‐shaped GQDs charged biopolymers is found generate series hierarchical structures that exhibit aggregation‐induced fluorescence quenching change...