A5060071324- Advanced Fluorescence Microscopy Techniques
- Photoacoustic and Ultrasonic Imaging
- Nonlinear Optical Materials Studies
- 3D Printing in Biomedical Research
- Optical Coherence Tomography Applications
- Surface Roughness and Optical Measurements
- Advanced optical system design
- Cellular Mechanics and Interactions
- Photochromic and Fluorescence Chemistry
- Advanced Biosensing Techniques and Applications
- Bone Tissue Engineering Materials
- Orthopedic Surgery and Rehabilitation
- Anesthesia and Neurotoxicity Research
- Tissue Engineering and Regenerative Medicine
- Muon and positron interactions and applications
- Mesenchymal stem cell research
- Hematopoietic Stem Cell Transplantation
- Acoustic Wave Resonator Technologies
- Neuroinflammation and Neurodegeneration Mechanisms
- Pluripotent Stem Cells Research
- Analytical Chemistry and Sensors
- Electrospun Nanofibers in Biomedical Applications
- Liquid Crystal Research Advancements
- Nanoplatforms for cancer theranostics
- Gastrointestinal Tumor Research and Treatment
Politecnico di Milano
2018-2025
Curculio Institute
2024
Abstract Non‐linear excitation microscopy offers several advantages for in‐vivo imaging compared to conventional confocal techniques. However, tissue penetration can still be an issue due scattering and spherical aberrations induced on focused beams by the tissue. The use of low numerical aperture objectives pass through outer layers skin, together with high dioptric power microlenses implanted close observation volume, beneficial reduction optical aberrations. Here, Fibroblast cell culture...
The process of angiogenesis plays a pivotal role in skin regeneration, ensuring the provision nutrients and oxygen to nascent tissue, thanks formation novel microvascular networks supporting functional tissue regeneration. Unfortunately, most current therapeutic approaches for regeneration lack vascularization, required promote effective angiogenesis. Thus,
The obtainment of innovative models recalling complex tumour architectures and activities in vitro is a challenging drive the understanding pathology molecular bases, yet crucial passage to identification targets for advanced oncotherapy. Cell environment recapitulation by 3D scaffolding gravitational unloading cell cultures represent powerful means biomimicry process, but their simultaneous adoption has consistently been explored only latest decade. Here, an unprecedented bioengineering...
Tissue histopathology, based on hematoxylin and eosin (H&E) staining of thin tissue slices, is the gold standard for evaluation immune reaction to implant a biomaterial. It lengthy costly procedures that do not allow longitudinal studies. The use non-linear excitation microscopy in vivo, largely label-free, has potential overcome these limitations. With this purpose, we develop validate an implantable microstructured device assessment implanted biomaterial label-free. device, shaped as...
Abstract The biocompatibility assessment of biomaterials or the dynamic response implanted constructs entails inflammatory events primary reflected in cell behavior at microcirculatory system. Current protocols are based on histopathology which over 40 years old and require sacrifice a huge number laboratory animal with an unsustainable ethical burden research. Intravital microscopy techniques actually used to study implantation outcomes real time. However, no device providing specific...
Introduction: An innovative approach to the treatment of tendon injury or degeneration is given by engineered grafts, made available through development bioreactors that generate tissue in vitro, replicating vivo conditions. This work aims at design a bioreactor capable applying stimulation cyclic strain on cell constructs promote production bioartificial with mechanical and biochemical properties resembling those native tissue. Methods: The system was actuated an electromagnet...
In the context of biomaterials, small-molecules and drugs testing, intravital microscopy allows to quantify in-vivo immune reaction, reducing number laboratory animals required statistically validate product. However, fluorescence is affected by limited tissue penetration due light scattering optical aberrations, induced on focused beams, animal surrounding implant. this framework, we developed a system microlenses coupled microscaffolds, both incorporated in miniaturized imaging window. The...
Tissue histopathology, reliant on costly and time-consuming hematoxylin eosin (H&E) staining of thin tissue slices, faces limitations. Label-free non-linear optical microscopy in vivo presents a solution, allowing work fresh samples. Implantable microstructures prove effective for systematic longitudinal studies immunological responses to biomaterials using label-free microscopy. Employing two-photon laser polymerization, we implanted matrix 3D lattices the chorioallantoic membrane chicken...
Long-term culture of primary lymphocytes and hematopoietic stem progenitor cells (HSPCs) is pivotal to their expansion study. Furthermore, genetic engineering the above-mentioned human has several safety needs, including requirement efficient
Specimen-induced aberrations limit the penetration depth of standard optical imaging techniques in vivo, mainly due to propagation high NA beams a non-homogenous medium. Overcoming these limitations requires complex systems and techniques. Implantable micro-optics can be solution tissue induced spherical but order implanted they need have reduced complexity, offering surface host immune reaction. Here, we design, fabricate test single micro-optical element with dioptric power (up 1.4 air)....
Specimen-induced aberrations limit the penetration depth of standard optical imaging techniques in vivo, mainly due to propagation high NA beams a non-homogenous medium. Overcoming these limitations requires complex systems and techniques. Implantable micro-optics can be solution tissue induced spherical but order implanted they need have reduced complexity, offering surface host immune reaction. Here, we design, fabricate test single micro-optical element with dioptric power (up 1.4 air)....
Specimen-induced aberrations limit the penetration depth of standard optical imaging techniques in vivo, mainly due to propagation high NA beams a non-homogenous medium. Overcoming these limitations requires complex systems and techniques. Implantable micro-optics can be solution tissue induced spherical but order implanted they need have reduced complexity, offering surface host immune reaction. Here, we design, fabricate test single micro-optical element with dioptric power (up 1.4 air)....
Non-linear excitation microscopy offers superior in-vivo imaging but faces challenges in deep tissue. High numerical aperture beams suffer spherical aberrations, while tissue scattering impacts image quality. To address this, we propose implantable microlenses for precise focusing below the skin lab animals. By using low lasers, avoid aberrations induced by high NA objectives. Our study presents various microlens designs differing size, shape, and fabrication methods, all on glass or...
Specimen-induced aberrations limit the penetration depth of standard optical imaging techniques in vivo, mainly due to propagation high NA beams a non-homogenous medium. Overcoming these limitations requires complex systems and techniques. Implantable micro-optics can be solution tissue induced spherical aberrations, but order implanted, they need have reduced complexity, offering lower surface host immune reaction. Here, we design, fabricate, test single micro-optical element with dioptric...
Brand new biomaterials, intended to be used on humans, must undergo in vivo quantification standardized, expensive and unethical procedures mainly based histopathological analysis, from dissections, as defined by the ISO 10993 normative set. The aim is prove biomaterials biocompatibility. There exist no methods intravital microscopy able satisfy requirements both reducing number of employed animals related costs. We developed a miniaturized imaging window, Microatlas, which allows...
The current protocols for biocompatibility assessment of biomaterials, based on histopathology, require the sacrifice a huge number laboratory animals with an unsustainable ethical burden and remarkable cost. Intravital microscopy techniques can be used to study implantation outcomes in real time though limited capabilities quantification longitudinal studies, mainly restricted by light penetration spatial resolution deep tissues. We present outline first tests novel chip which aims enable...
The human brain is the most complex organ in biology. This complexity due to number and intricate connections of cells has so far limited development vitro models for basic applied research. We decided create a new, reliable, cost-effective system based on Nichoid, 3D microscaffold microfabricated by two-photon laser polymerization technology. investigated whether these devices can an environment allowing manipulation, monitoring, functional assessment mixed population vitro. With this aim,...
Abstract Non-linear excitation microscopy offers several advantages for in-vivo imaging compared to conventional confocal techniques. However, tissue penetration can still be an issue due scattering and spherical aberrations induced on focused beams by the tissue. The use of low numerical aperture objectives pass through outer layers skin, together with high dioptric power microlenses implanted close observation volume, beneficial reduction optical aberrations. Here, we develop test...
We describe several bioengineered cell models developed by our group.We develop tools for culture allowing fluorescence diagnostics on the cellularised constructs cultured within, both in 3D and prolonged times extending to weeks.These proved able recapitulate vitro slowly developing biological processes such as regeneration of a cartilaginous tissue cartilage cells, formation bone metastasis breast cancer instruction adaptive immune cells occurs lymphnode, neuroprotective effect...