A5065608502- Child and Adolescent Psychosocial and Emotional Development
- Racial and Ethnic Identity Research
- Nitric Oxide and Endothelin Effects
- Mental Health Treatment and Access
- Hemoglobinopathies and Related Disorders
- Pharmacological Effects and Toxicity Studies
- Ion channel regulation and function
- Organ Transplantation Techniques and Outcomes
- Renal Transplantation Outcomes and Treatments
- Immune Response and Inflammation
- Diversity and Career in Medicine
- Organ Donation and Transplantation
- Connexins and lens biology
- Iron Metabolism and Disorders
- Migration, Health and Trauma
- Child Abuse and Trauma
- Cardiac electrophysiology and arrhythmias
- Eating Disorders and Behaviors
- Renin-Angiotensin System Studies
- Telemedicine and Telehealth Implementation
- Amino Acid Enzymes and Metabolism
- Hormonal Regulation and Hypertension
- Attention Deficit Hyperactivity Disorder
- Down syndrome and intellectual disability research
- Maternal Mental Health During Pregnancy and Postpartum
Carilion Clinic
2024
Washington University in St. Louis
2021-2024
Shanghai East Hospital
2024
Rogers (United States)
2024
University of California, Los Angeles
2023-2024
Neurobehavioral Systems
2023-2024
UCLA Health
2024
American Academy of Child and Adolescent Psychiatry
2023
Jacksonville College
2019
Mayo Clinic in Florida
2019
The biosynthesis of cachectin/TNF is largely regulated at a post-transcriptional level. Bacterial endotoxin, which strongly induces production, thus seems to elicit least some its effects by altering the macrophage cytoplasmic milieu. It has previously been shown that 3'-untranslated TTATTTAT element present in numerous cytokines and proto-oncogenes capable repressing translation mRNA molecules it represented. Using constructs CAT coding sequence followed varying segments region, we now...
Tumor necrosis factor (TNF) is a protein hormone implicated in the development of septic shock and other pathologic states. However, complexities inherent detecting TNF synthesis by individual tissues have left precise origins this undefined. In addition, possibility that localized production may contribute to pathogenesis organ-specific diseases such as type I diabetes has not been explored vivo. We developed transgenic mouse line bearing reporter gene construct which coding sequence...
Endothelial function is impaired in aging because of a decrease NO bioavailability. This may be, part, attributable to increased arginase activity, which reciprocally regulates synthase (NOS) by competing for the common substrate, L-arginine. However, high Km (>1 mmol/L) compared with NOS (2 20 micromol/L) seemingly makes direct competition substrate unlikely. One mechanisms exerts its effects posttranslational modification through S-nitrosylation protein cysteines. We tested hypothesis that...
Nitric oxide (NO) is an important mediator of diverse physiological and pathological responses. To determine whether NO production can be induced in skeletal muscle, we stimulated C2C12 mouse muscle myocytes with putative inducers nitric synthase (NOS). Neither lipopolysaccharide (LPS), interleukin-1 alpha (IL-1), tumor necrosis factor-alpha (TNF), nor interferon-gamma (IFN) was able to stimulate nitrite by cells when administered alone. However, combinations IFN either TNF or IL-1 resulted...
Although tumor necrosis factor (TNF) is a major mediator of endotoxic shock, the normal function TNF that has preserved this protein throughout mammalian evolution remains unknown. If serves role in development or homeostasis, it must be produced under physiologic conditions. To determine whether secretion occurs animals, and to define tissue sources protein, we prepared reporter construct which coding sequence introns are replaced by chloramphenicol acetyltransferase (CAT) sequence. This...
This study was performed with Dahl salt-sensitive (DS) and salt-resistant (DR) rats to detect differences in cardiovascular hemodynamics renal responses that might be involved initiating salt-induced hypertension DS rats. The effects of 4 weeks 8% NaCl diet were studied conscious, male DR which vascular urinary catheters had been previously implanted. Results compared those obtained from control groups on 1% diet. salt did not develop hypertension, cardiac output blood volume unchanged;...
We have prepared a construct (designated CATTNF) in which the mouse TNF (cachectin) coding sequence is replaced by encoding chloramphenicol acetyltransferase (CAT), with preservation of promoter and 3'-untranslated sequences known to be important regulation gene expression. When activated LPS, permanently transfected RAW 264.7 (mouse macrophage) cells synthesize large quantities CAT. Unlike itself, CAT nonsecreted quite stable macrophage cytoplasm. Fewer than 1,000 LPS-induced macrophages...
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There is a great need for evidence-based antiracism interventions targeting mental health clinicians to help mitigate disparities in racially and ethnically minoritized groups.
Background Incidence rates of autism, attention-deficit/hyperactivity disorder (ADHD), and gender dysphoria (GD) are rising not only in the general population, but particularly among children, adolescents, young adults with eating disorders (EDs). While ED have risen during COVID pandemic, trends co-occurring ADHD, GD yet to be investigated detail or at scale by way large electronic medical record data. Objectives To investigate EDs years prior COVID-19 pandemic. Methods We utilized a...