A5085575023- Neuroinflammation and Neurodegeneration Mechanisms
- Estrogen and related hormone effects
- Menopause: Health Impacts and Treatments
- Nitric Oxide and Endothelin Effects
- Phytoestrogen effects and research
- Atrial Fibrillation Management and Outcomes
- Heart Failure Treatment and Management
- Neuroscience and Neuropharmacology Research
- Protein Hydrolysis and Bioactive Peptides
- Antioxidant Activity and Oxidative Stress
- Neurological Disease Mechanisms and Treatments
- Telomeres, Telomerase, and Senescence
- Cerebrovascular and Carotid Artery Diseases
- Biochemical effects in animals
- Hormonal and reproductive studies
- Mitochondrial Function and Pathology
- Sexual function and dysfunction studies
- Infant Nutrition and Health
- Cardiac Ischemia and Reperfusion
- MicroRNA in disease regulation
- Sleep and Wakefulness Research
- GDF15 and Related Biomarkers
- Antimicrobial Peptides and Activities
- Free Radicals and Antioxidants
- Axon Guidance and Neuronal Signaling
Instituto de Investigación Sanitaria La Fe
2014-2025
Universitat de València
2014-2025
Universitat de Miguel Hernández d'Elx
2011
Instituto de Agroquímica y Tecnología de Alimentos
2006
Consejo Superior de Investigaciones Científicas
2006
Leitat Technological Center
2001
Background and Purpose— Ischemic stroke continues to be one of the main causes death worldwide. Inflammation accounts for a large part damage in this pathology. The cannabinoid type 2 receptor (CB2R) has been proposed have neuroprotective properties neurological diseases. Therefore, our aim was determine effects activation CB2R on infarct outcome ischemia-induced brain expression classic alternative markers macrophage/microglial activation. Methods— Swiss wild-type knockout male mice were...
Cerebral ischemia is a devastating disease that affects many people worldwide every year. The neurodegenerative damage as consequence of oxygen and energy deprivation, to date, has no known effective treatment. ischemic insult followed by an inflammatory response involves complex interaction between cells molecules which play role in the progression towards cell death. However, there presently matter controversy over whether inflammation could either be involved brain or necessary part...
In recent years, evidence of the existence cellular senescence in central nervous system has accumulated. ischemic stroke, been suggested as an unidentified pathophysiological mechanism, prompting research into neuroprotective potential senolytic drugs. This study aims to provide spatio-temporal brain following stroke and elucidate involved pathways cell types. We focused on most established markers senescence: cycle arrest (p16, p21); lysosomal activity (senescence-associated...
Abstract As phytoestrogens are postulated as being neuroprotectants, we assessed the hypothesis that dietary isoflavone‐type neuroprotective against ischemic stroke. Transient focal cerebral ischemia (90 min) was induced by middle artery occlusion (MCAO) following intraluminal thread technique, both in rats fed with soy‐based diet and isoflavone‐free diet. Cerebro‐cortical laser‐Doppler flow (cortical perfusion, CP), arterial blood pressure, core temperature, P a O 2 , CO pH glycemia were...
Estrogens account for gender differences in the incidence and outcome of stroke, but it remains unclear to what extent neuroprotective effects estrogens are because parenchymal or vascular actions. Because reproductive steroids have vasoactive properties, authors assessed mechanisms action 17-beta-estradiol rabbit isolated basilar artery. Cumulative doses (0.3 micromol/L 0.1 mmol/L) induced concentration-dependent relaxation that was larger than carotid artery, male female KCl-precontracted...
Growth factors promote cell growth and survival protect the brain from developing injury after ischemia. In this article, authors examined whether transforming factor-alpha (TGF-alpha) was protective in transient focal ischemia alteration of cerebral circulation involved. Rats received intraventricular TGF-alpha (50 ng, either split into 2 doses given 30 minutes before middle artery occlusion (MCAO), or 1 dose MCAO) vehicle. were subjected to 1-hour intraluminal MCAO blood flow recorded...
The involvement of plasma membrane glutamate transporters (EAATs - excitatory aminoacid transporters) in the pathophysiology ischemia has been widely studied, but little is known about role vesicular (VGLUTs) ischemic process. We analyzed expression VGLUT1-3 cortex and caudate-putamen rats subjected to transient middle cerebral artery occlusion. Western blot immunohistochemistry revealed an increase VGLUT1 signal until 3 days reperfusion followed by a reduction 7 after insult. By contrast,...
Brain preconditioning (PC) refers to a state of transient tolerance against lethal insult that can be evoked by prior mild event. It is thought PC may induce different pathways responsible for neuroprotection, which involve the attenuation cell damage pathways, including apoptotic death. In this context, p53 stress sensor accumulates during brain ischemia leading neuronal The murine double minute 2 gene (MDM2), p53-specific E3 ubiquitin ligase, main cellular antagonist p53, mediating its...
Aging is a major risk factor for cerebral infarction. Since cellular senescence intrinsic to aging, we postulated that stroke-induced might contribute neural dysfunction. Adult male Wistar rats underwent 60-minute middle artery occlusion and were grouped according 3 reperfusion times: 24 hours, 3, 7 days. The biomarkers of senescence: 1) accumulation the lysosomal pigment, lipofuscin; 2) expression cell cycle arrest markers p21, p53, p16INK4a; 3) senescence-associated secretory phenotype...
A selection of lactoferricin B (LfcinB)-related peptides with an angiotensin I-converting enzyme (ACE) inhibitory effect have been examined using in vitro and ex vivo functional assays. Peptides that were analyzed included a set sequence-related antimicrobial hexapeptides previously reported two representative LfcinB-derived peptides. In assays hippuryl-l-histidyl-l-leucine (HHL) I as substrates allowed us to select hexapeptides, PACEI32 (Ac-RKWHFW-NH2) PACEI34 (Ac-RKWLFW-NH2), also peptide,...
Including sex is of paramount importance in preclinical and clinical stroke researches, molecular studies dealing depth with differences pathophysiology are needed. To gain insight into the dimorphism ischaemic rat cerebral cortex, male female adult rats were subjected to transient middle artery occlusion. The expression neuroglobin (Ngb) other functionally related molecules involved steroid signalling (oestrogen androgen receptors), steroidogenesis (StAR, TSPO aromatase) autophagic activity...
Abstract Because neuroprotection in stroke should be revisited the era of recanalisation, present study analysed potential neuroprotective effect selective oestrogen receptor modulator, bazedoxifene acetate ( BZA ), an animal model diabetic ischaemic that mimics thrombectomy combined with adjuvant administration a putative neuroprotectant. Four weeks after induction diabetes (40 mg kg ‐1 streptozotocin, i.p.), male Wistar rats were subjected to transient middle cerebral artery occlusion...