A5072087636- Adipose Tissue and Metabolism
- Genetic Mapping and Diversity in Plants and Animals
- Regulation of Appetite and Obesity
- Vitamin C and Antioxidants Research
- Genetic and phenotypic traits in livestock
- Adipokines, Inflammation, and Metabolic Diseases
- Stress Responses and Cortisol
- Skin and Cellular Biology Research
- Fibroblast Growth Factor Research
- Wnt/β-catenin signaling in development and cancer
- Birth, Development, and Health
- Genomics and Phylogenetic Studies
- ATP Synthase and ATPases Research
- Endoplasmic Reticulum Stress and Disease
- Metabolism, Diabetes, and Cancer
- Genetic Associations and Epidemiology
- Bacteriophages and microbial interactions
- Cancer Research and Treatments
- Cell death mechanisms and regulation
- Cardiovascular Disease and Adiposity
- Microtubule and mitosis dynamics
- Circadian rhythm and melatonin
- Mycobacterium research and diagnosis
- FOXO transcription factor regulation
- Genetics, Aging, and Longevity in Model Organisms
Wake Forest University
2021-2024
Centre College
2019-2020
Despite the successes of human genome-wide association studies, causal genes underlying most metabolic traits remain unclear. We used outbred heterogeneous stock (HS) rats, coupled with expression data and mediation analysis, to identify quantitative trait loci (QTLs) candidate gene mediators for adiposity, glucose tolerance, serum lipids, other traits. Physiological were measured in 1,519 male HS liver adipose transcriptomes >410 rats. Genotypes imputed from low-coverage whole-genome...
As the number of cancer survivors increases and risk accidental radiation exposure rises, there is a pressing need to characterize delayed effects develop medical countermeasures. Radiation has been shown damage adipose progenitor cells increase liver fibrosis, such that it predisposes patients developing metabolic-associated fatty disease (MAFLD) insulin resistance. The these conditions compounded by global rise diets rich in carbohydrates fats. persistently signaling cascade transforming...
Despite the obesity crisis in United States, underlying genetics are poorly understood. Our lab previously identified Keratinocyte-associated protein 3, Krtcap3, as a candidate gene for adiposity through genome-wide association study outbred rats, where increased liver expression of Krtcap3 correlated with decreased fat mass. Here we seek to confirm that affects traits. To do so, developed an vivo whole-body knock-out (KO) rat model. Wild-type (WT) and KO rats were placed onto high-fat (HFD)...
We previously identified Keratinocyte-associated protein 3, Krtcap3, as a novel adiposity gene, but subsequently found that its impact on may depend environmental stress. To more thoroughly understand the connection between adiposity, and stress, we exposed wild-type (WT) Krtcap3 knock-out (KO) rats to chronic stress then measured behavioral outcomes. KO displayed lower basal than WT under control conditions exhibited metabolic responses exposure. Specifically, stress-exposed gained weight,...
We previously identified keratinocyte-associated protein 3,
Abstract Background Whole genome sequencing promises to revolutionize our ability link genotypic and phenotypic variation in a wide range of model non-model species. Results Here we describe the isolation characterization novel mycobacteriophage named BGlluviae that grows on Mycobacterium smegmatis mc 2 155. normally produces turbid plaques but spontaneous clear plaque was also recovered. The genomic DNA from pure populations phage mutant were sequenced. A single substitution, at amino acid...
has been linked to both obesity and major depressive disorder (MDD). Our lab identified a protein-coding variant in the 2
Abstract Objective Adenylate cyclase 3 ( Adcy3 ) has been linked to both obesity and major depressive disorder. We identified a protein‐coding variant in the transmembrane (TM) helix of rats; similar variants have humans. This study investigates role TM adiposity behavior. Methods mutated domain mut/mut created heterozygous knockout +/− Wistar Kyoto (WKY) rats. Wild‐type, , rats were fed high‐fat diet for 12 weeks. measured body weight, fat mass, glucose tolerance, food intake, metabolism,...
Abstract We previously identified Keratinocyte-associated protein 3, Krtcap3, as an obesity-related gene in female rats where a whole-body Krtcap3 knock-out (KO) led to increased adiposity compared wild-type (WT) controls when fed high-fat diet (HFD). sought replicate this work better understand the function of but were unable reproduce phenotype. In current work, WT ate more prior study, with corresponding increases body weight and fat mass, while there no changes these measures KO females...
Programmed cell death, or apoptosis, is a cellular pathway by which individual cells self-destruct for the benefit of organism. In this practical paper, we describe laboratory exercises with an inquiry-based learning (IBL) approach in undergraduate students compared apoptosis among different types cultured cells. Ultraviolet (UV) radiation was used to induce mouse primary and Chinese hamster ovary (CHO) tumor Students hypothesized that, because evade CHO would exhibit less Treated control...
Abstract Objective Despite the obesity crisis in United States, underlying genetics are poorly understood. Our lab previously identified Keratinocyte-associated protein 3, Krtcap3, as a candidate gene for adiposity where increased expression of Krtcap3 correlated with decreased fat mass. Here we seek to confirm that affects traits. Methods We developed an vivo whole-body knock-out (KO) rat model. Wild-type (WT) and KO rats were placed onto high-fat or low-fat diet at six weeks age maintained...
While obesity is an increasingly prevalent disease, genetic susceptibility poorly understood and more research sorely needed. Our lab has sought to confirm Keratinocyte-associated protein 3 ( Krtcap3) as a novel adiposity gene characterize its role using in vivo whole-body knock-out (KO) model on the Wistar-Kyoto (WKY) rat strain.We obtained conflicting food intake results between two studies conducted before after COVID-19 pandemic, which brought along drastic environmental changes animal...
Abstract We previously identified Keratinocyte-associated protein 3 , Krtcap3 as a novel adiposity gene but subsequently found that its impact on may depend environmental stress. To more thoroughly understand the connection between adiposity, and stress, we exposed wild-type (WT) knock-out (KO) rats to chronic stress then measured behavioral outcomes. KO displayed lower basal than WT under control conditions exhibited expected responses exposure. Specifically, stress-exposed gained weight,...
Abstract Obesity is a growing epidemic associated with range of comorbidities, including anxiety and depression. Genetics environmental factors such as diet contribute to both adiposity anxiety/depression. Heterogeneous stock (HS) rats are an outbred colony useful for genetic mapping complex traits. We have previously shown that HS male exhibit worsened metabolic behavioral health in response high fat (HFD). This study aims determine if females similar interacts background. measured multiple...
Although obesity rates have been steadily climbing the last few decades, genetics underlying disease are poorly understood. Previous work in our lab identified Keratinocyte-associated protein 3(Krtcap3) as a candidate gene for adiposity. To characterize role of Krtcap3in adiposity, we developed an vivowhole-body knock-out (KO) rat model Krtcap3,hypothesizing that KO rats would greater adiposity compared to wild-type (WT) rats. In first vivostudy, primarily occurring before COVID-19 shut...
<p>Despite the successes of human genome-wide association studies, causal genes underlying most metabolic traits remain unclear. We used outbred heterogeneous stock (HS) rats, coupled with expression data and mediation analysis, to identify quantitative trait loci (QTLs) candidate gene mediators for adiposity, glucose tolerance, serum lipids, other traits. Physiological were measured in 1519 male HS liver adipose transcriptomes over 410 rats. Genotypes imputed from low coverage whole...
<p>Despite the successes of human genome-wide association studies, causal genes underlying most metabolic traits remain unclear. We used outbred heterogeneous stock (HS) rats, coupled with expression data and mediation analysis, to identify quantitative trait loci (QTLs) candidate gene mediators for adiposity, glucose tolerance, serum lipids, other traits. Physiological were measured in 1519 male HS liver adipose transcriptomes over 410 rats. Genotypes imputed from low coverage whole...
Obesity poses a significant health burden in the United States, and investigating obesity-related genes remains an important area of research. A previous study from our lab used outbred heterogeneous stock (HS) rats to identify Keratinocyte-associated protein 3, (Krtcap3)as candidate gene within quantitative trait locus for adiposity. HS with Wistar-Kyoto (WKY) haplotype at this had increased liver expression Krtcap3, which correlated decreased retroperitoneal fat mass (RetroFat) serum...
Obesity is a growing epidemic associated with range of comorbidities, including heart disease, diabetes, and mental health. Studies suggest underlying genetics may be contributing to both adiposity anxiety/depression, in addition environmental factors, such as diet, both. To better understand the shared genetic factors involved obesity health, we used Heterogeneous Stock (HS) rat, population created from 8 inbred strains outbred maximize diversity, resulting genetically phenotypically unique...