A5101887111- Genomics and Phylogenetic Studies
- TGF-β signaling in diseases
- Alkaline Phosphatase Research Studies
- Mitochondrial Function and Pathology
- Plant Molecular Biology Research
- RNA Research and Splicing
- Muscle metabolism and nutrition
- Signaling Pathways in Disease
- Enzyme Structure and Function
- Chromosomal and Genetic Variations
- Protein Kinase Regulation and GTPase Signaling
- Folate and B Vitamins Research
- RNA modifications and cancer
- Glycogen Storage Diseases and Myoclonus
- S100 Proteins and Annexins
- Endoplasmic Reticulum Stress and Disease
- Impact of Light on Environment and Health
- Chromium effects and bioremediation
- Oral health in cancer treatment
- Glioma Diagnosis and Treatment
- Muscle Physiology and Disorders
- Pancreatic function and diabetes
- HIV Research and Treatment
- Amphibian and Reptile Biology
- Animal Virus Infections Studies
University of Arizona
2024
University of Kentucky
2020-2021
Western Kentucky University
2019-2020
Albert Einstein College of Medicine
2013
University of Dundee
1980-2005
University of California, Davis
1983
Methods were developed for quantifying protein phosphatases-1, 2B and 2C in cell extracts, thse procedures exploited to determine their tissue subcellular distributions. In addition, the contribution of each enzyme total phosphatase activity skeletal muscle liver extracts towards nine proteins involved control glycogen metabolis, glycolysis/gluconeogenesais, fatty acid synthesis cholesterol was assessed. Each present at significant concentrations muscle, heart liverr, brain adipose tissue,...
The MgATP-dependent phosphorylase phosphatase was found to have a broad substrate specificity. Its activity against all phosphoproteins tested dependent upon preincubation with the activating factor FA and MgATP. enzyme dephosphorylated inactivated kinase inhibitor 1, activated glycogen synthase acetyl-CoA carboxylase. Glycogen at similar rates whether it had been phosphorylated by cyclic-AMP-dependent protein kinase, or 3. also catalysed dephosphorylation of ATP citrate lyase, initiation...
Protein phosphatase-2B was purified from extracts of rabbit skeletal muscle by a procedure that involved fractionation with ammonium sulphate, chromatography on DEAE-Sepharose, poly(ethylene glycol), gel filtration Sephadex G-200 (Mr= 98000 ± 4000), Affi-Gel Blue and affinity calmodulin-Sepharose. The enzyme 35000-fold in senven days an overall yield 0.5%. α-subunit phosphorylase kinase, protein phosphatase inhibitor-1 the myosin P-light chain were dephosphorylated similar kinetic constants....
Abstract Increased exposure to light pollution perturbs physiological processes through misalignment of daily rhythms at the cellular and tissue levels. Effects artificial light-at-night (ALAN) on diel properties immunity are currently unknown. We therefore tested effects ALAN patterns cytokine gene expression, as well key hormones involved with regulation immunity, in zebra finches ( Taeniopygia guttata ). Circulating melatonin corticosterone, mRNA expression levels pro- IL-1β , IL-6 )...
Telomeres form the ends of linear chromosomes and usually comprise protein complexes that bind to simple repeated sequence motifs are added 3' DNA by telomerase reverse transcriptase (TERT). One primary functions attributed telomeres is solve "end-replication problem" which, if left unaddressed, would cause gradual, inexorable attrition sequences from chromosome and, eventually, loss viability. Telomere-binding proteins also protect 5' exonuclease action, disguise double-strand break repair...
A stable copper-resistant mutant of Saccharomyces cerevisiae took up less copper than the wild-type. The use protoplasts showed that decreased uptake depended on changed membrane transport properties and not alterations in cell wall.
The transforming growth factor beta (TGF-β) family of modulators play critical roles in tissue development and maintenance. Recent data suggest that individual TGF-β isoforms (TGF-β1, -β2 -β3) have overlapping yet distinct biological actions target cell specificities, both developing adult tissues. TGF-β3 isoform was purified to homogeneity from natural recombinant sources characterized by laser desorption mass spectrometry, protein sequencing, amino acid analysis activity. the major...
Abstract Background Whole genome sequencing promises to revolutionize our ability link genotypic and phenotypic variation in a wide range of model non-model species. Results Here we describe the isolation characterization novel mycobacteriophage named BGlluviae that grows on Mycobacterium smegmatis mc 2 155. normally produces turbid plaques but spontaneous clear plaque was also recovered. The genomic DNA from pure populations phage mutant were sequenced. A single substitution, at amino acid...
Abstract Transfer RNAs (tRNA) are crucial adaptor molecules between messenger RNA (mRNA) and amino acids. Recent evidence in plants suggests that dicistronic tRNA-like structures also act as mobile signals for mRNA transcripts to move distant tissues. Co-transcription is not a common feature the plant nuclear genome and, few cases where polycistronic have been found, they include non-coding species, such small nucleolar microRNAs. It known, however, extent which of tRNA mRNAs expressed...
Analysis of factors contributing to high affinity antibody-protein interactions provides insight into natural antibody evolution, and guides the design antibodies with new or enhanced function. We previously studied interaction between D5 its target, a designed protein based on HIV-1 gp41 known as 5-Helix, model system [Da Silva, G. F.; Harrison, J. S.; Lai, R., Biochemistry, 2010, 49, 5464-5472]. Antibody represents an interesting case study because it is derived from VH1-69 germline...
Epithelial- and haematopoietic-cell growth-inhibitory activities have been identified in the conditioned medium of human peripheral neuroepithelioma cell line A673. An A673-cell-derived activity was previously fractionated into two distinct components which inhibited proliferation carcinoma leukaemia cells culture. One inhibitory shown to comprise interleukin-1 alpha (IL-1 alpha). Here, we purified homogeneity a growth epithelial vitro. Using combination protein-sequence analysis mass...
Abstract Transfer RNAs (tRNA) are crucial adaptor molecules between messenger RNA (mRNA) and amino acids. Recent evidence in plants suggests that dicistronic tRNA-like structures also act as mobile signals for mRNA transcripts to move distant tissues. Co-transcription is not a common feature the plant nuclear genome and, few cases where polycistronic have been found, they include non-coding species such small nucleolar microRNAs. It known, however, extent which of tRNA mRNAs expressed...