A5071278482- Genetic Mapping and Diversity in Plants and Animals
- Plant Stress Responses and Tolerance
- Rice Cultivation and Yield Improvement
- Plant Molecular Biology Research
- Molecular Biology Techniques and Applications
- Plant responses to water stress
- Plant nutrient uptake and metabolism
- GABA and Rice Research
- Mitochondrial Function and Pathology
- Plant Genetic and Mutation Studies
- Photosynthetic Processes and Mechanisms
- Genetics and Plant Breeding
- Research in Cotton Cultivation
- CRISPR and Genetic Engineering
- Hemoglobin structure and function
- Insect Resistance and Genetics
- Endoplasmic Reticulum Stress and Disease
- Spectroscopy and Chemometric Analyses
- Leaf Properties and Growth Measurement
- ATP Synthase and ATPases Research
- Rural development and sustainability
- Seed Germination and Physiology
- Genomics and Phylogenetic Studies
- Transgenic Plants and Applications
- MicroRNA in disease regulation
Texas Tech University
2018-2024
Louisiana State University
2023-2024
Meiji University
2022
International Rice Research Institute
2013-2016
Abstract Sub-QTLs and multiple intra-QTL genes are hypothesized to underpin large-effect QTLs. Known QTLs over gene families, biosynthetic pathways or certain traits represent functional gene-clusters of the same ontology (GO). Gene-clusters containing different GO have not been elaborated, except in silico as coexpressed within Here we demonstrate requirement for full impact QTL qDTY 12.1 on rice yield under drought. Multiple evidences presented need transcription factor ‘no apical...
Abstract LCIA (low CO2-inducible protein A) is a chloroplast envelope associated with the CO2-concentrating mechanism of green alga Chlamydomonas reinhardtii. postulated to be HCO3– channel, but previous studies were unable show that was actively transporting bicarbonate in planta. Therefore, activity investigated more directly two heterologous systems: an Escherichia coli mutant (DCAKO) lacking both native carbonic anhydrases and Arabidopsis (βca5) missing plastid anhydrase βCA5. Neither...
Quantitative reverse transcription PCR (qRT-PCR) has been routinely used to quantify gene expression level. This technique determines the of a target by comparison an internal control uniformly expressed among samples analyzed. The reproducibility and reliability results depend heavily on reference genes used. To achieve successful analyses for drought tolerance studies in rice, selection should be based consistency across variables. We aimed provide that would consistent different tissues,...
The ATP-driven bicarbonate transporter 1 (BCT1) from Synechococcus is a four-component complex in the cyanobacterial CO2-concentrating mechanism. BCT1 could enhance photosynthetic CO2 assimilation plant chloroplasts. However, directing its subunits (CmpA, CmpB, CmpC, and CmpD) to three chloroplast sub-compartments highly complex. Investigating integration into Nicotiana benthamiana chloroplasts revealed promising targeting strategies using transit peptides intermembrane space protein Tic22...
The phenomenon of transgressive segregation, where a small minority recombinants are outliers relative to the range parental phenotypes, is commonly observed in plant breeding populations. While this has been attributed complementation and epistatic effects, physiological developmental synergism involved have not fully illuminated by QTL mapping approach alone, especially for stress-adaptive traits involving highly complex interactions. By systems-level profiling IR29 × Pokkali recombinant...
There is a widespread consensus that drought will mostly affect present and future agriculture negatively. Generating drought-tolerant crops thus high priority. However complicated the underlying genetic regulatory networks for differences in plant performance under stress are, they would be reflected straightforward primary metabolites. This because metabolites such as amino acids sugars form building blocks of all pathways processes growth, development, reproduction, environmental...
Soil salinity severely hampers agricultural productivity. Under salt stress, excess Na+ accumulation causes cellular damage and plant growth retardation, membrane transporters play central roles in uptake exclusion to mitigate these adverse effects. In this study, we performed sos1 suppressor mutant (named sup) screening uncover potential genetic interactors of SOS1 additional tolerance mechanisms. Map-based cloning sequencing identified a group mutants harboring dominant gain-of-function...
Abstract Oryza officinalis is an accessible alien donor for genetic improvement of rice. Comparison across a representative panel species showed that the wild O . and cultivated sativa ssp. japonica have similar cold tolerance potentials. The possibility either distinct or mechanisms are involved in low temperature responses each was addressed by comparing their transcriptional networks. General similarities were supported shared transcriptomic signatures indicative equivalent metabolic,...
The abilities to mobilize and/or sequester excess ions within and outside the plant cell are important components of salt-tolerance mechanisms. Mobilization sequestration Na + involves three transport systems facilitated by plasma membrane H /Na antiporter (SOS1), vacuolar (NHX1), /K transporter in vascular tissues (HKT1). Many these mechanisms conserved across kingdom. While Gossypium hirsutum (upland cotton) is significantly more salt-tolerant relative other crops, critical factors...
Genetic novelties are important nucleators of adaptive speciation. Transgressive segregation is a major mechanism that creates genetic with morphological and developmental attributes confer advantages in certain environments. This study examined the morpho-developmental physiological profiles recombinant inbred lines (RILs) from salt-sensitive IR29 salt-tolerant Pokkali rice, representing total range salt tolerance including outliers at both ends spectrum. Morpho-developmental were...
The ratio of Na+ and K+ is an important determinant the magnitude toxicity osmotic stress in plant cells. Traditional analytical approaches involve destructive tissue sampling chemical analysis, where real-time observation spatio-temporal experiments across genetic or breeding populations unrealistic. Such approach can also be very inaccurate prone to erroneous biological interpretation. Analysis by Hyperspectral Imaging (HSI) emerging non-destructive alternative for tracking nutrient status...
Abstract Introgression of major-effect QTLs is an important component rice breeding for yield-retention under drought. While largely effective, the maximum potentials such have not been consistent across genetic backgrounds. We hypothesized that synergism or antagonism with additive-effect peripheral genes background could either enhance undermine QTL effects. To elucidate molecular underpinnings interaction, we dissected qDTY12.1 synergy numerous in context network rewiring By integrative...
<title>Abstract</title> Transgressive segregation offers a non-reductionist strategy for breeding crops with novel mechanisms complex adaptive traits through the omnigenic paradigm. While this phenomenon has been linked to complementation and epistatic effects, contribution of epigenome is unknown. We examined well-characterized recombinant inbred population (F<sub>9</sub>) <italic>Oryza sativa</italic> (rice) derived from parents wide genetic contrast (IR29/<italic>indica</italic> x...
The ATP-driven bicarbonate transporter 1 (BCT1), a four-component complex in the cyanobacteria CO 2 concentrating mechanism, could enhance photosynthetic assimilation plant chloroplasts. However, directing its subunits (CmpA, CmpB, CmpC and CmpD) to three chloroplast sub-compartments is highly complex. Investigating BCT1 integration into Nicotiana benthamiana chloroplasts revealed promising targeting strategies using transit peptides from intermembrane space protein Tic22 for correct CmpA...
The inherent complexity of adaptive mechanisms to salinity stress necessitates novel approaches plant breeding. In rice, wide introgression is an approach that yet be fully exploited, especially from the not-so-distant species related cultivars. We explored genetic combining potential AA-genome wild Oryza rufipogon, closest progenitor cultivated sativa ssp. japonica, uncover physiological novelties for tolerance created by transgressive effects. Comprehensive evaluation a set chromosome...
Abstract Transgressive segregation is common in plant breeding populations, where a small minority of recombinants are outliers relative to parental phenotypes. While this phenomenon has been attributed complementation and epistatic effects, the physiological, biochemical, molecular bases have not fully illuminated. By systems-level scrutiny IR29 x Pokkali recombinant inbred population rice, we addressed hypothesis that novel salt tolerance phenotypes created by positive or negative coupling...
ABSTRACT Toxic ions begin to accumulate in tissues of salt-stressed plants after the initial osmotic shock. In glycophytes, ability mobilize or sequester excess define tolerance mechanisms. Mobilization and sequestration Na + involves three transport mechanisms facilitated by plasma membrane H /Na antiporter (SOS1), vacuolar (NHX1), /K transporter vascular (HKT1). While cultivated Gossypium hirsutum (upland cotton) is significantly more tolerant salinity relative other crops, critical...