A5081107633- Prion Diseases and Protein Misfolding
- Animal Disease Management and Epidemiology
- Nanoparticles: synthesis and applications
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Animal Ecology and Behavior Studies
- Metallurgy and Material Science
- Zoonotic diseases and public health
- Advanced biosensing and bioanalysis techniques
- Insect Resistance and Genetics
- Microbial infections and disease research
- Selenium in Biological Systems
- Advanced Nanomaterials in Catalysis
- Anodic Oxide Films and Nanostructures
- Health, Environment, Cognitive Aging
- Soybean genetics and cultivation
- Food Allergy and Anaphylaxis Research
- Environmental Toxicology and Ecotoxicology
- Trace Elements in Health
- RNA regulation and disease
- Heavy Metal Exposure and Toxicity
- Vector-Borne Animal Diseases
University of Minnesota
2023-2025
University of Minnesota System
2025
University of Wisconsin–Madison
2021-2024
University of Kentucky
2016-2020
Plant (United States)
2020
Duke University
2016
Abstract Environmental surveillance can allow early detection of diseases, which increases management options and improve disease trajectories. Chronic wasting (CWD) in cervids is a significant prion that has been spreading across North America since the 1960s, leading to cervid population declines concern from hunters state wildlife agencies. White‐tailed deer have unique breeding season behaviour called scraping, where they deposit urine saliva at shared sites. Since both these fluids...
Chronic wasting disease (CWD) is a highly contagious, fatal neurodegenerative caused by infectious prions (PrP
Sensitive and specific antemortem diagnostic tests are a prerequisite for effective management of chronic wasting disease (CWD). Paired with readily accessible samples that accurately reflect CWD status, the real-time quaking-induced conversion (RT-QuIC) assay has potential to enable more surveillance interventions. We evaluated feasibility RT-QuIC as test using 6-mm ear tissue biopsies from elk (Cervus canadensis). First, we effect spatial location on seeding activity. observed an punch...
Abstract Eliminating supplemental feeding is a common regulatory action within chronic wasting disease (CWD) management zones. These regulations target the potential for increased animal‐animal contact and environmental contamination with CWD prions. Prions, causative agent of CWD, have been detected on feeder surfaces in CWD‐positive, captive deer facilities but not among free‐ranging populations, information relative risk transmission at anthropogenic natural food sources limited. In this...
Chronic wasting disease (CWD) is a fatal neurodegenerative affecting cervids. CWD caused by infectious prions, which can enter the environment through bodily fluids or carcasses of infected animals. Prions be stored, remain in both soil and water for many years, transported hydrologically, possibly expanding geographic range transmission. In order to better predict hydrological prion transport, we investigated how protein (PrPCWD) partitions persists environmental waters. We performed PrPCWD...
Prion diseases, including chronic wasting disease (CWD), are caused by prions, which misfolded aggregates of normal cellular prion protein. Prions possess many characteristics that distinguish them from conventional pathogens, in particular, an extraordinary recalcitrance to inactivation and a propensity avidly bind surfaces. In middle late stages CWD, prions begin accumulating cervid muscle tissues. Those features collectively create scenarios occupational hazards arise for workers...
Abstract Chronic wasting disease (CWD) is a fatal neurodegenerative caused by infectious prions (PrP CWD ) affecting cervids. Circulating PrP in blood may pose risk for indirect transmission way of hematophagous ectoparasites acting as mechanical vectors. Cervids can carry high tick infestations and exhibit allogrooming, common defense strategy between conspecifics. Ingestion ticks during allogrooming expose naïve animals to CWD, if harbor . This study investigates whether...
In this study, we investigated chitosan/dsRNA polyplex nanoparticles as RNAi agents in the nematode Caenorhabditis elegans. By measurement of an easily observed phenotype and uptake fluorescently labeled dsRNA, demonstrate that are considerably more effective at gene knockdown on a whole body concentration basis than naked dsRNA. Further, show introduce dsRNA into cells via different mechanism canonical sid-1 sid-2 pathway. Clathrin-mediated endocytosis is likely main mechanism. Finally,...
ABSTRACT Chronic wasting disease (CWD) prions cause fatal neuropathies in farmed and free-ranging cervids. The deposition of natural humanmade environmental components has been implicated as a major mechanism mediating CWD spread wild captive populations. Prions can be deposited the environment through excreta, tissues, carcasses from pre-clinical clinical animals. Furthermore, burial CWD-positive animals may reduce but not completely mitigate prion into surrounding environment. Here, we...
Abstract Prion diseases, including chronic wasting disease (CWD), are caused by prions, which misfolded aggregates of normal cellular prion protein. Prions possess many characteristics that distinguish them from conventional pathogens, in particular, an extraordinary recalcitrance to inactivation and a propensity avidly bind surfaces. In mid late stages CWD, prions begin accumulating cervid muscle tissues. These features collectively create scenarios where occupational hazards arise for...
Chronic wasting disease (CWD) is a fatal prion affecting cervids (deer, elk, moose). Current methods to monitor individual state include highly invasive antemortem rectal biopsy or postmortem brain biopsy. Efficient, sensitive, and selective testing of populations would increase knowledge the dynamics CWD epizootics as well provide means track progression into previously unaffected areas. Here, we analyzed presence prions in skin samples from two easily accessed locations (ear belly) 30...
Chitosan/dsRNA polyplex nanoparticles have shown great potential as insect biocontrol agents. Here, we show that their gene silencing capacity altered by environmental variables.
The rapid geographic spread of chronic wasting disease (CWD) in white-tailed deer (WTD;
Abstract Summary Fluorophore-assisted seed amplification assays (F-SAAs), such as real-time quaking-induced conversion (RT-QuIC) and fluorophore-assisted protein misfolding cyclic (F-PMCA), have become indispensable tools for studying in neurodegenerative diseases. However, analyzing data generated by these techniques often requires complex time-consuming manual processes. Additionally, the lack of standardization F-SAA analysis presents a significant challenge to interpretation...
RNA interference is a promising crop protection technology that has seen rapid development in the past several years. Here, we investigated polyamino acid biopolymers, inorganic nanomaterials, and hybrid organic–inorganic nanomaterials for delivery of dsRNA efficacy gene knockdown using model nematode Caenorhabditis elegans. Using an oral route delivery, are able to approximate how will be delivered environment. Of materials investigated, only Mg–Al layered double-hydroxide nanoparticles...
Environmental routes of transmission contribute to the spread prion diseases chronic wasting disease deer and elk scrapie sheep goats. Prions can persist in soils other environmental matrices remain infectious for years. bind avidly common soil mineral montmorillonite, such binding dramatically increase oral transmission. Decontamination captive facilities natural habitats requires inactivation agents that are effective when prions bound microparticles. Here, we investigate free...
Prion diseases such as scrapie, bovine spongiform encephalopathy (BSE), and chronic wasting disease (CWD) affect domesticated wild herbivorous mammals. Animals afflicted with CWD, the transmissible of cervids (deer, elk, moose), shed prions into environment, where they may persist remain infectious for years. These environmental in soil, be transported surface waters, or assimilated plants. Environmental sampling is an emerging area TSE research can provide more information about prion fate...
Abstract Motivation Fluorophore-assisted seed amplification assays (F-SAAs), such as real-time quaking-induced conversion (RT-QuIC) and fluorophore-assisted protein misfolding cyclic (F-PMCA), have become indispensable tools for studying in neurodegenerative diseases. However, analyzing data generated by these techniques often requires complex time-consuming manual processes. Additionally, the lack of standardization F-SAA analysis presents a significant challenge to interpretation...
ABSTRACT Chronic wasting disease (CWD) is a highly contagious, fatal neurodegenerative caused by infectious prions (PrP CWD ) affecting wild and captive cervids. Although experimental feeding studies have demonstrated in feces of crows ( Corvus brachyrhynchos ), coyotes Canis latrans cougars Puma concolor the role scavengers predators epidemiology remains poorly understood. Here we applied real-time quaking-induced conversion (RT-QuIC) assay to detect PrP from cervid consumers, advance...