A5028922293- Prion Diseases and Protein Misfolding
- Animal Disease Management and Epidemiology
- Metallurgy and Material Science
- Fire effects on ecosystems
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Virus-based gene therapy research
- Animal Ecology and Behavior Studies
- Veterinary Oncology Research
- Wildlife Ecology and Conservation
- Animal Virus Infections Studies
- Identification and Quantification in Food
- Bacteriophages and microbial interactions
- Amphibian and Reptile Biology
- Animal Behavior and Reproduction
- Coccidia and coccidiosis research
- Toxoplasma gondii Research Studies
- Nutrition, Genetics, and Disease
- Zoonotic diseases and public health
- Global Public Health Policies and Epidemiology
- Rabies epidemiology and control
- Avian ecology and behavior
- Primate Behavior and Ecology
- Food Allergy and Anaphylaxis Research
- RNA regulation and disease
University of Minnesota
2006-2025
University of Minnesota System
2025
Animal and Plant Health Inspection Service
2013
Minnesota Department of Natural Resources
2013
Jiangsu Provincial Center for Disease Control and Prevention
2013
Abstract Chronic wasting disease (CWD) is a prion circulating in wild and farmed cervid populations throughout North America (United States Canada), Europe (Finland, Norway, Sweden), South Korea. CWD long-term threat to all hunting heritage, with the potential cause substantial economic losses across multiple sectors. In America, farming industries focused on processing consumption of white-tailed deer (WTD) venison are particularly vulnerable contamination, as millions WTD consumed...
Throughout North America, chronic wasting disease (CWD) has emerged as perhaps the greatest threat to wild cervid populations, including white-tailed deer (WTD; Odocoileus virginianus). White-tailed are most sought-after big game species across America with populations of various subspecies in nearly all Canadian provinces, contiguous US, and Mexico. Documented CWD cases have dramatically increased WTD range since mid-1990s, Minnesota, US. surveillance free-ranging other mainly depends upon...
Chronic wasting disease (CWD) has been identified in 30 states the United States, four provinces Canada, and recently emerged Scandinavia. The association of CWD prions with environmental materials such as soil, plants, surfaces may enhance persistence prion infectivity environment exacerbating transmission. Identifying quantifying is significant for monitoring transmission control. A systematic method quantification from associated materials, however, does not exist. In this study, we...
Chronic wasting disease (CWD) is a affecting cervids and caused by prions accumulating as pathogenic fibrils in lymphoid tissue the central nervous system. Approaches for detecting CWD historically relied on antibody-based assays. However, recent advancements protein amplification technology provided foundation new class of diagnostic tools. In particular, real-time quaking-induced conversion (RT-QuIC) has rapidly become feasible option diagnosis. Despite its increased usage CWD-focused...
Abstract Prion diseases, including chronic wasting disease (CWD), are caused by prions, which misfolded aggregates of normal cellular prion protein. Prions possess many characteristics that distinguish them from conventional pathogens, in particular, an extraordinary recalcitrance to inactivation and a propensity avidly bind surfaces. In mid late stages CWD, prions begin accumulating cervid muscle tissues. These features collectively create scenarios where occupational hazards arise for...
Abstract Eliminating supplemental feeding is a common regulatory action within chronic wasting disease (CWD) management zones. These regulations target the potential for increased animal‐animal contact and environmental contamination with CWD prions. Prions, causative agent of CWD, have been detected on feeder surfaces in CWD‐positive, captive deer facilities but not among free‐ranging populations, information relative risk transmission at anthropogenic natural food sources limited. In this...
Chronic wasting disease (CWD) is a fatal neurodegenerative affecting cervids. CWD caused by infectious prions, which can enter the environment through bodily fluids or carcasses of infected animals. Prions be stored, remain in both soil and water for many years, transported hydrologically, possibly expanding geographic range transmission. In order to better predict hydrological prion transport, we investigated how protein (PrPCWD) partitions persists environmental waters. We performed PrPCWD...
Prion diseases, including chronic wasting disease (CWD), are caused by prions, which misfolded aggregates of normal cellular prion protein. Prions possess many characteristics that distinguish them from conventional pathogens, in particular, an extraordinary recalcitrance to inactivation and a propensity avidly bind surfaces. In middle late stages CWD, prions begin accumulating cervid muscle tissues. Those features collectively create scenarios occupational hazards arise for workers...
In many species, young animals learn about various breeding patches in one year and use what they have learned to settle a promising patch the next. Common loons (Gavia immer) seem good candidates for such prospecting as prebreeders displaced breeders intrude frequently into territories defended by monogamous pairs yet engage no extrapair copulations. We tested 3 hypotheses loons. The permanent attributes hypothesis gained little support we found consistent differences quality between...
Prion diseases are untreatable fatal transmissible neurodegenerative that affect a wide range of mammals, including humans, and caused by PrP
Abstract We tested blood samples from domestic and captive farmed animals in Minnesota, USA, to determine exposure severe fever with thrombocytopenia syndrome virus Heartland-like virus. found antibodies against nucleoproteins 10%–18% of cattle, sheep, goats, deer, elk 24 Minnesota counties.
Abstract We tested blood samples from domestic and captive farmed animals in Minnesota, USA, to determine exposure severe fever with thrombocytopenia syndrome virus Heartland-like virus. found antibodies against nucleoproteins 10%–18% of cattle, sheep, goats, deer, elk 24 Minnesota counties.
Osteosarcoma is a devastating bone cancer that disproportionally afflicts children, adolescents, and young adults. Standard therapy includes surgical tumor resection combined with multiagent chemotherapy, but many patients still suffer from metastatic disease progression. Neoadjuvant systemic oncolytic virus (OV) has the potential to improve clinical outcomes by targeting primary sites inducing durable antitumor immune responses. Here we describe first evaluation of neoadjuvant...
Chronic wasting disease (CWD) is a transmissible prion of the cervidae family. ELISA and IHC tests performed postmortem on medial retropharyngeal lymph nodes (RPLN) or obex are considered diagnostic gold standards for detection. However, differences in CWD transmission, stage infection, pathogenesis, strain can limit performance. To overcome these uncertainties, we used Bayesian statistics to assess accuracy RT-QuIC, an increasingly amplification assay, diagnose tonsil (TLN), parotid (PLN)...
Diagnostic tools for the detection of protein-misfolding diseases (i.e., proteopathies) are limited. Gold nanoparticles (AuNPs) facilitate sensitive diagnostic techniques via visual color change identification a variety targets. In parallel, recently developed quaking-induced conversion (QuIC) assays leverage protein-amplification and fluorescent signaling accurate misfolded proteins. Here, we combine AuNP QuIC technologies amplified prion proteins from tissues wild white-tailed deer...
A 22-month-old, female red deer (Cervus elaphus) was submitted to the University of Minnesota Veterinary Diagnostic Laboratory for necropsy and chronic wasting disease (CWD) testing. The found positive abnormal prion protein in obex retropharyngeal lymph node by immunohistochemical staining. Microscopic lesions spongiform encephalopathy staining patterns intensity were similar those CWD-positive elk experimentally infected deer.
A novel coccidian parasite from the kidney of big brown bats (Eptesicus fuscus) is described. This (Nephroisospora eptesici nov. gen., n. sp.) was associated with a generally mild, focal or multifocal, well-demarcated cortical renal lesion less than 1 mm in diameter. The represented cystic, dilated tubules hypertrophied tubular epithelial cells and present kidneys 29 590 bats. Numerous parasites various stages development were within cyst lumina. Oocysts collected cystic tubules....
Infectious prions are resistant to degradation and remain infectious in the environment for several years. Chronic wasting disease (CWD) has been detected cervids inhabiting North America, Nordic countries, South Korea. CWD-prion spread is partially attributed carcass transport disposal. We employed a forensic approach investigate an illegal dump site connected with CWD-positive herd. integrated anatomic, genetic, prion amplification methods discover remains from six white-tailed deer...
The rapid geographic spread of chronic wasting disease (CWD) in white-tailed deer (WTD;
ABSTRACT Throughout North America, chronic wasting disease (CWD) has emerged as perhaps the greatest threat to wild cervid populations, including white-tailed deer ( Odocoileus virginianus ). White-tailed are most sought after big game species across America with populations of various subspecies in nearly all Canadian provinces, contiguous USA, and Mexico. Documented CWD cases have dramatically increased range since mid-1990s, Minnesota. surveillance free-ranging other mainly depends upon...
We describe significant brain, craniofacial, and dental lesions in a free-ranging wolf (Canis lupus) involved human attack. On postmortem examination, the presented asymmetric atrophy bone remodeling affecting mandible, incisive, maxilla, lacrimal, palatine, frontal, ethmoid bones. There was an asymmetrical skeletal malocclusion abnormalities including rotated, malpositioned, partially erupted teeth, odontogenic cyst associated with unerupted canine tooth. Brain changes were bilateral loss...
Livers of 25 common garter snakes (Thamnophis sirtalis) from Minnesota (2015–16) were analyzed for heavy metals by inductively coupled plasma atomic emission spectroscopy. Dry weight mean concentrations (ranges µg/g) arsenic, 118.94 (0.26–1245.56); cadmium, 1.44 (0.15–7.59); lead, 0.21 (<0.10–0.78); and mercury, 0.30 (<0.5–2.25).