A5021029460- Prion Diseases and Protein Misfolding
- Neurological diseases and metabolism
- Animal Disease Management and Epidemiology
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Vagus Nerve Stimulation Research
- Neuroscience of respiration and sleep
- Mycobacterium research and diagnosis
- Adenosine and Purinergic Signaling
- RNA regulation and disease
The University of Texas Health Science Center at Houston
2023-2024
Pontificia Universidad Católica de Chile
2021
We describe chronic wasting disease (CWD) prion detection in raw and cooked meat from a CWD-positive elk. found limited zoonotic potential CWD prions those products. Nonetheless, risk for transmission to humans is still unclear, monitoring of circulating emerging strains warranted.
ABSTRACT Chronic wasting disease (CWD) prions cause fatal neuropathies in farmed and free-ranging cervids. The deposition of natural humanmade environmental components has been implicated as a major mechanism mediating CWD spread wild captive populations. Prions can be deposited the environment through excreta, tissues, carcasses from pre-clinical clinical animals. Furthermore, burial CWD-positive animals may reduce but not completely mitigate prion into surrounding environment. Here, we...
Abstract Chronic wasting disease (CWD) is a prion affecting farmed and free-ranging cervids. CWD rapidly expanding across North America its mechanisms of transmission are not completely understood. Considering that cervids commonly afflicted by nasal bot flies, we tested the potential these parasites to transmit CWD. Parasites collected from naturally infected white-tailed deer were evaluated for their content using protein misfolding cyclic amplification (PMCA) technology bioassays. Here,...
Allergic asthma is a chronic airway inflammatory response to different triggers like inhaled allergens. Excessive ATP in fluids from patients with considered an signal and important autocrine/paracrine modulator of physiology. Here, we investigated the deleterious effect increased extracellular (eATP) concentration on mucociliary clearance (MCC) effectiveness determined role releasing channels during inflammation ovalbumin (OVA)-sensitized mouse model. Our allergic model exhibited high...
Prion diseases result from the misfolding of physiological prion protein (PrP
Chronic wasting disease (CWD) is a prion affecting cervids. CWD diagnosis conducted through enzyme-linked immunosorbent assay (ELISA) and immunohistochemistry (IHC) in retropharyngeal lymph nodes. Unfortunately, these techniques have limited sensitivity against the biomarker (CWD-prions). Two vitro amplification techniques, real-time quaking-induced conversion (RT-QuIC) protein misfolding cyclic (PMCA), shown promise detecting CWD-prions tissues bodily fluids. Recent studies demonstrated...
Prion diseases are a group of fatal neurodegenerative disorders affecting several mammals including humans, cattle, cervids, sheep, goats, and others [1-3].These driven by the misfolding cellular prion protein (PrP C ) into β-sheet rich structures.The diseaseassociated prion, termed PrP Sc , is prone to aggregation as well neurotoxic [4][5][6].Prion can be associated with mutations in gene (PRNP), induced exposure prion-contaminated materials, or arise sporadically [3,7].A relevant area...
Using a prion amplification assay, we identified prions in tissues from wild pigs (Sus scrofa) living areas of the United States with variable chronic wasting disease (CWD) epidemiology. Our findings indicate that scavenging swine could play role disseminating CWD and therefore influence its epidemiology, geographic distribution, interspecies spread.
Chronic wasting disease (CWD) is a prion affecting cervids. Confirmatory testing of CWD currently performed postmortem in obex and lymphoid tissues. Extensive evidence demonstrates the presence infectious prions feces CWD-infected deer using vitro prion-amplification techniques bioassays. In experimental conditions, this has been achieved as soon 6-month post-inoculation, suggesting sample type candidate for antemortem diagnosis. present study, we optimized detection CWD-prions fecal samples...