A5102740513- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- RNA modifications and cancer
- Cancer-related gene regulation
- Cardiovascular Function and Risk Factors
- Signaling Pathways in Disease
- Cardiac Fibrosis and Remodeling
- Autophagy in Disease and Therapy
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Cell death mechanisms and regulation
- Photoreceptor and optogenetics research
- Neurological Disorders and Treatments
- Synthesis and Biological Evaluation
- Phagocytosis and Immune Regulation
- Ubiquitin and proteasome pathways
- Cancer Research and Treatments
- Adipose Tissue and Metabolism
- Inflammasome and immune disorders
- Endoplasmic Reticulum Stress and Disease
- CRISPR and Genetic Engineering
- Cardiomyopathy and Myosin Studies
- Cancer-related molecular mechanisms research
- Receptor Mechanisms and Signaling
- Photosynthetic Processes and Mechanisms
Fujian Medical University
2025
Zhengzhou University
2013-2024
Knowledge Unlatched (Germany)
2001
Introduction: Mycoplasma pneumoniae is a bacterial infection and the primary causative agent for pneumonia, which poses significant health burden strains medical industry. Elderly individuals, people with compromised immune systems, those lung diseases, sickle cell disease, children are particularly vulnerable. Method: To replace traditional methods, it essential to develop an accurate method detecting pneumonia early diagnosis improved treatment outcomes. In this research, highly sensitive...
The expression of BECLIN1 is significantly reduced in non‑small cell lung cancer (NSCLC) compared with non‑cancerous tissue. However, the role unclear. Using RNA interference (RNAi) technique present study investigated effect knockdown on growth and proliferation A549 human line. target site for RNAi was designed lentivirus vector small interfering (si)RNA constructed according to encoding sequence mRNA BECLIN1. cells were transfected siRNA virus against detected by reverse transcription...
Although Beclin 1 has been demonstrated to exert an important role in cell autophagy during carcinogenesis, its biological function lung cancer yet be fully elucidated. A previous study by our laboratory identified that knockdown of promoted growth and inhibited apoptosis the A549 line. In present study, a lentiviral expression vector was constructed, line established with steady 1. Furthermore, effect overexpression on invasion apoptosis, changes activities apoptosis‑associated caspases‑3...
Junctophilins (JPs), a protein family of the junctional membrane complex, maintain close conjunction between cell surface and intracellular membranes in striate muscle cells mediating crosstalk extracellular Ca2+ entry release. The small-conductance -activated K+ channels are activated by calcium play an essential role cardiac action potential profile. Molecular mechanisms regulation SK still uncertain. Here, we sought to determine whether there is functional interaction junctophilin type 2...
Abstract Aim To analyze and compare the mRNA N 6 -methyladenosine modifications in transverse aortic constriction induced mice hearts normal hearts. Materials methods Colorimetric quantification was used to probe changes m A total RNA. The expression of A-related enzymes analyzed via qRT-PCR western blotting. RNA-seq MeRIP-seq were performed identify genes with differences or transcriptome profile. Results Compared control group, TAC group exhibited higher methylation levels. FTO WTAP...
The aim of this study was to investigate the effects GSK-3β/NF-κB pathway on integrin-associated protein (CD47) expression after myocardial infarction (MI) in rats. An MI Sprague Dawley rat model established by ligating left anterior descending coronary artery. rats were divided into three groups: Sham, MI, and SB + (SB216763) groups. Immunohistochemistry used observe changes cardiac morphology. A significant reduction sizes fibrotic scars observed group compared that group. SB216763...
Abstract Small‐conductance Ca 2+ ‐activated K + channel subtype2 (SK2) are stable macromolecular complexes that regulate myocardial excitability and homeostasis. Junctophilin‐2 (JP2) is a membrane‐binding protein, which provides functional crosstalk by physically linking with the cell‐surface intracellular ion channels. We previously demonstrated MORN domain of JP2 interacts SK2 However, roles in regulating precise subcellular localization molecular modulation have not yet been incompletely...
The interaction between junctophilin-2 (JPH2) and ryanodine receptor type 2 (RyR2) regulated Ca2+ signaling in mouse cardiomyocytes. However, their exact remains unclear. This study elucidates the JPH2 with RyR2 using co-immunoprecipitation of cardiac sarcoplasmic reticulum vesicles. Additionally, a glutathione S-transferase (GST) pull-down analysis was performed to investigate physical fragments. interacted C terminus protein can receptors. Confocal immunofluorescence imaging indicated that...
目的: 探讨腹主动脉缩窄小鼠在向心衰发展的过程中心脏结构和功能的动态变化。方法: 健康雄性昆明小鼠,随机分为模型组、假手术组和对照组。模型组采用腹主动脉缩窄的方法制备慢性心力衰竭小鼠模型,假手术组只分离出腹主动脉但不结扎,对照组不做任何处理。模型组组内分为2周组、4周组、6周组和8周组,每组10只。观察各组小鼠行为学表现、心电图、超声心动图和心肌组织病理学的变化。结果: 模型组与对照组相比:模型组小鼠术后2周开始出现行为学改变并且仅有2周组小鼠出现IVSS降低(P＜0.05);术后4周开始出现病理性J波、EF降低(P＜0.05)、IVSD增加(P＜0.05)和心肌损伤;术后6周开始出现LVPWD和LVPWS增加(P＜0.05);术后8周开始出现LV mass corrected增加(P＜0.05)。各组小鼠心率、R幅值、T幅值、ST段、PR间期、QT间期、QTc等数据差异均无显著性。结论: 腹主动脉缩窄导致小鼠出现心衰的过程中出现了EF降低、室间隔肥厚、病理性J波、左室后壁肥厚以及左室质量增加等变化。.
Objective To investigate the effect of JP2 knockdown on expression Caveolin-1 in H9c2 cardiomyocytes. Methods The siRNA recombinant adenovirus vector targeting rat gene (Ad-JP2-siRNA) and control (Ad-NC-siRNA) were constructed used to transfect cells. In each group cells, real-time PCR was detect level mRNA, Western blotting combined with surface biotin protein, total protein membrane immunocytochemistry examine subcellular localization cells. Results At 48 h, compared group,...
To investigate the expression of 2-type small conductance-Ca2+-activating-K+ (SK2) channel protein in hypertensive rat myocardial cells.Twelve healthy adult male SD rats were randomly divided into control group (n=5) and experimental (n=7). The injected intraperitoneally with N'-nitro-L-arginine (L-NNA 15 mg/(kg·d))while isometrical normal saline(15 ml/(kg·d )). body weight, blood pressure electrocardiogram measured every week. After 4 weeks, sacrificed to obtain hearts, SK2 myocardium was...
Abstract Background: Ryanodine receptor type 2 (RyR2) mediate Ca 2+ release from the endoplasmic and sarcoplasmic reticulum (ER SR), which is involved in peripheral coupling of mouse cardiomyocytes, thereby plays an important role cardiac contraction. Junctophilin-2 (JPH2, JP2) anchored to plasma membrane (PM) membranes ER SR, modulates intracellular handling through regulation RyR2. However, potential RyR2 binding region JPH2 poorly understood. Methods: The interaction with was studied...