A5068415774- Milk Quality and Mastitis in Dairy Cows
- Probiotics and Fermented Foods
- Streptococcal Infections and Treatments
- Antimicrobial Resistance in Staphylococcus
- Microbial infections and disease research
- Animal health and immunology
- Digestive system and related health
- Biosensors and Analytical Detection
- Reproductive Physiology in Livestock
- Animal Disease Management and Epidemiology
- Animal Behavior and Welfare Studies
- Neonatal and Maternal Infections
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Viral Infections and Vectors
- Parasitic Infections and Diagnostics
- Fatty Acid Research and Health
- Vector-Borne Animal Diseases
- Effects of Environmental Stressors on Livestock
- Animal Diversity and Health Studies
- Infant Nutrition and Health
- Ruminant Nutrition and Digestive Physiology
- Plant Pathogenic Bacteria Studies
- Salmonella and Campylobacter epidemiology
- Viral gastroenteritis research and epidemiology
University of Lisbon
2014-2025
Centre for Research in Anthropology
2015-2024
Instituto Nacional de Investigação Agrária e Veterinária
2022
International Iberian Nanotechnology Laboratory
2015
Instituto de Engenharia de Sistemas e Computadores Microsistemas e Nanotecnologias
2015
University of Glasgow
2007-2010
Royal College of Physicians and Surgeons of Glasgow
2009
Instituto de Investigação Científica Tropical
2007
Flow cytometers have been optimized for use in portable platforms, where cell separation, identification and counting can be achieved a compact modular format. This feature combined with magnetic detection, magnetoresistive sensors integrated within microfluidic channels to detect magnetically labelled cells. work describes platform in-flow detection of cells magneto-resistive based cytometer. In particular, we present an example the validation as counter that identifies quantifies...
The use of recycled manure solids (RMS) as cow bedding in dairy farms poses concerns due to its potential harbor pathogenic and antimicrobial-resistant bacteria. This study evaluated the impact RMS supplementation with biochar at three concentrations (2.5%, 5%, 10%) on bacterial counts antimicrobial resistance virulence profiles Escherichia coli Enterococcus isolates. results show that did not promote a significant reduction numbers. Overall, there were no changes isolates’ or profiles, none...
ABSTRACT A custom-designed microarray containing 220 virulence genes of Streptococcus pyogenes (group [GAS]) was used to test group C dysgalactiae subsp. (GCS) field strains causing bovine mastitis and or G equisimilis (GCS/GGS) isolates from human infections, with the latter being for comparative purposes, presence genes. All all carried a fraction (23% 39%, respectively). The encoding streptolysin S, glyceraldehyde-3-phosphate dehydrogenase, plasminogen-binding M-like protein PAM,...
Group B Streptococcus (GBS) is a host-generalist species, most notably causing disease in humans and cattle. However, the differential adaptation of GBS to its two main hosts, risk animal human infection remain poorly understood. Despite improvements control measures across Europe, still one causative agents bovine mastitis Portugal. Here, by whole-genome analysis 150 isolates we discovered that single CC61 clone spreading throughout Portuguese herds since at least early 1990s, having...
Bovine mastitis is the most costly disease for dairy farmers and frequent reason use of antibiotics in cattle; thus, control measures to detect prevent are crucial farm sustainability. The aim this study was develop validate a sensitive method magnetically Streptococcus agalactiae (a Group B streptococci) uberis raw milk samples. Mastitic samples were collected aseptically from 44 cows with subclinical mastitis, 11 Portuguese farms. Forty-six quarter selected based on bacterial...
Neonatal calves are commonly affected by diarrhea caused different pathogens, but not always bacteria. Yet, antibiotics routinely used as a treatment to an unknown extent. It was our goal survey antibiotic use for the of neonatal calf in countries and identify influencing factors. A total 873 farmers veterinarians Austria, Belgium, Portugal, Scotland participated voluntary online survey. The data were analyzed using classification regression tree analyses chi2 tests. Overall, 52.5%...
Up to half of quarter milk samples submitted for mastitis diagnosis are culture-negative results or lead identification coagulase-negative staphylococci Corynebacterium bovis in conventional culturing, the so-called minor pathogens. The interpretation and usefulness these terms udder animal health management is limited, even though amount resources spent relatively high. This work aimed test two methods analysis with goal increasing detection intramammary In first study, 783 were processed...
Streptococcus dysgalactiae subsp. (SDSD) has been considered a strict animal pathogen. Nevertheless, the recent reports of human infections suggest niche expansion for this subspecies, which may be consequence virulence gene acquisition that increases its pathogenicity. Previous studies reported presence genes pyogenes phages among bovine SDSD (collected in 2002–2003); however, identity these mobile genetic elements remains to clarified. Thus, study aimed characterize isolates collected...
Background: Making a clinical diagnosis of pericarditis in cattle is difficult and additional diagnostic tests are needed to evaluate with suspected pericarditis. Serum cardiac troponin I (cTnI) concentrations increased pericarditis, but the utility measuring serum cTnI remains unclear. Objectives: To determine if can be used differentiate from other disorders noncardiac thoracic diseases. Animals: Seventy‐seven clinically diseased 19 healthy control cattle. Methods: were measured using an...
To maximise the return on capital invested in milking parlour, largest number of cows should be milked gently and completely shortest possible time. Bimodal milk flow overmilking negatively influence efficiency removal process teat health. This observational study had objective investigating prevalence bimodal overmilking, determining which individual farm-related variables are associated with these occurrences, association yield short- long-term changes. Twenty-one farms were visited once...
The records of 116 cattle suffering from cardiac disease were examined retrospectively. On the basis results postmortem examinations there 52 cases endocarditis, 39 pericarditis and 25 congenital defects. most useful clinical tool for differentiating between these conditions was auscultation heart. characterised by muffled heart sounds, endocarditis defects a murmur. Endocarditis could be differentiated presence jugular pulse, venous distension, oedema, reduced appetite, pain polyarthritis,...
Staphylococcus (S.) aureus is a common infectious agent of bovine chronic mastitis, disease that difficult to eradicate.The abilities Staphylococci be internalized and form biofilm can contribute host immunological defence evasion subsequently impairs antimicrobial therapy.The invasive capability six S. field isolates with different biofilmforming profiles was compared in vitro using mammary epithelial cell line.This further confirmed primary cultures fluorescent rRNA probes against...