A new species of the Campylobacter genus is described, isolated from preputial mucosa bulls (Bos taurus). The five isolates obtained exhibit characteristics Campylobacter, being Gram-negative non-motile straight rods, oxidase positive, catalase negative and microaerophilic. Phenotypic nucleotide sequence analysis 16S rRNA hsp60 genes demonstrated that these belong to a novel within Campylobacter. Based on gene phylogenetic analysis, most related are C. ureolyticus, blaseri corcagiensis....

Bovine mastitis is the most costly disease for dairy farmers and frequent reason use of antibiotics in cattle; thus, control measures to detect prevent are crucial farm sustainability. The aim this study was develop validate a sensitive method magnetically Streptococcus agalactiae (a Group B streptococci) uberis raw milk samples. Mastitic samples were collected aseptically from 44 cows with subclinical mastitis, 11 Portuguese farms. Forty-six quarter selected based on bacterial...

Rabbit haemorrhagic disease (RHD) is a major threat to domestic and wild European rabbits. Presently, in Europe, the caused mainly by virus 2 (RHDV2/b or Lagovirus europaeus GI.2), origin of which still unclear, as no RHDV2 reservoir hosts were identified. After emergence 2010, viral RNA was detected few rodent species. Furthermore, found cause some hare species resembling rabbits, evidencing ability cross barrier. In this study, through molecular, histopathologic, antigenic morphological...

Up to half of quarter milk samples submitted for mastitis diagnosis are culture-negative results or lead identification coagulase-negative staphylococci Corynebacterium bovis in conventional culturing, the so-called minor pathogens. The interpretation and usefulness these terms udder animal health management is limited, even though amount resources spent relatively high. This work aimed test two methods analysis with goal increasing detection intramammary In first study, 783 were processed...

Most of surgical site infections (SSI) are caused by commensal and pathogenic agents from the patient's microbiota, which may include antibiotic resistant strains. Pre-surgical asepsis skin is one preventive measures performed to reduce SSI incidence also resistance dissemination. However, in veterinary medicine there no agreement on biocide most effective. The aim this study was evaluate effectiveness two pre-surgical protocols dogs. A total 46 animals were randomly assigned for an protocol...

The objectives of this study were to compare the impact different coagulase-negative species (CNS) on udder health measured in terms individual quarter milk somatic cell count (SCC) and duration intramammary infection, get some insight into most likely routes infection for CNS species. This longitudinal observational was performed four farms that sampled at 4-week intervals a total 12 visits each. Quarters infected with followed through time samples being submitted bacteriological culture...

Multidrug-resistant (MDR) Klebsiella pneumoniae is considered as a major global concern by the World Health Organization. Evidence growing on importance of circulation MDR bacterial populations between animals and humans. Horses have been shown to carry commensal isolates this species can act human bacteria reservoirs. In study, we characterized an extended-spectrum β-lactamase (ESBL)-producing K. sequence type (ST) 348 isolate from horse, ST for first time reported in animal, using...

Previous studies revealed high levels of antimicrobial resistance (AMR) in loggerhead sea turtles (Caretta caretta), describing this species as prime reservoir antimicrobial-resistant bacteria. This study aimed to characterise, for the first time, AMR and virulence profiles Gram-negative bacteria isolated from 33 nesting island Maio, Cape Verde. Cloacal, oral, egg content swab samples (n = 99) were collected analysed using conventional bacteriological techniques. Shewanella putrefaciens,...

Bovine mastitis is the most costly disease for dairy farmers, hence, control measures to prevent it are crucial farm sustainability. Staphylococcus aureus considered a major pathogen because of its impact on milk quality and low cure rates. Prevention S. includes segregation infected animals, whilst treatment such animals should be performed longer time improve This makes identification quarters significant importance. The experiments reported in this research paper aimed develop validate...

Background Wildlife has been recently recognized as an environmental reservoir for antimicrobial resistance (AMR). However, less information on this topic is available in animals released back into the wild after rehabilitation wildlife facilities, compared with studies performed exclusively captive or free-ranging wildlife. This study aimed to evaluate potential influence of captivity and/or treatment while sloths AMR and virulence profiles sloths’ Enterobacterales . Methods Oral rectal...

Abstract Background Periodontal disease (PD) in dogs is prompted by the establishment of a polymicrobial biofilm at tooth surface and subsequent host inflammatory response. Several strategies may be used for PD control, including dental hygiene home care procedures, like toothbrushing, special diet chew toys that reduce plaque accumulation, or professional periodontal treatments. Aiming biogel composed nisin guar-gum was previously developed. This work aimed to establish an vitro model...

Although Corynebacterium bovis and coagulase-negative staphylococci are frequently the most commonly isolated bacteria from milk samples submitted for identification of pathogens causing intramammary infection, individual quarter somatic cell count (SCC) those is often low. The present study aimed at evaluating difference in bacteriology results sampled by standard technique (as recommended National Mastitis Council) use a teat cannula surpassing canal, since C. only found canal. Single were...

<title>Abstract</title> <bold>Background:</bold> Several studies detected high levels of antibiotic-resistance in loggerhead sea turtles (Caretta caretta) and pointed this species as prime reservoirs antibiotic-resistant bacteria carriers potentially pathogenic bacteria. This study aimed to characterize, for the first time, Gram-negative aerobic microbiota Cape Verdean subpopulation. Cloacal, oral egg content swab samples from 33 nesting loggerheads (n = 99) Island Maio were analysed...

&lt;p class="Pa5"&gt;&lt;span lang="EN-US"&gt;The widespread use of antimicrobials has lead to the emergence resistant bacteria one or more antibiotic, including new drugs like linezolid. This antimicrobial is first oxazolidinone group and soon after its approval in 2000, linezolid-resistant MRSA linezolid vancomycin-resistant enterococci have emerged. Several outbreaks been reported worldwide but, our knowledge, this report a isolated from dog Portugal. The animal arrived at Teaching...

Abstract Background The Biological Isolation and Containment Unit (BICU) is a subunit of the Teaching Hospital Faculty Veterinary Medicine University Lisbon, Portugal, for admission animals with confirmed infectious diseases or under clinical suspicion waiting diagnosis. As high-risk environment transmission agents, it extremely important to implement programs surveillance nosocomial microorganisms in these facilities. purpose this study was evaluate level bacterial contamination BICU...

Abstract This research paper aimed to evaluate the association between feeding waste milk calves and occurrence of antimicrobial multi-resistance by extended spectrum β-lactamase (ESBL) enzymes through determining their production E. coli isolates from 32 dairy farms. Among enzymes, ESBL provide resistance a wide variety β-lactam antimicrobials including penicillin 2nd, 3rd 4th generation cephalosporins. Feeding has been observed lead increased in faecal calves. In each farm included this...