A5054635912- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- Salmonella and Campylobacter epidemiology
- Escherichia coli research studies
- Vibrio bacteria research studies
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Antibiotics Pharmacokinetics and Efficacy
- Spectroscopy Techniques in Biomedical and Chemical Research
- Genomics and Phylogenetic Studies
- Mycobacterium research and diagnosis
- Antibiotic Use and Resistance
- Urinary Tract Infections Management
- Infections and bacterial resistance
- Pneumonia and Respiratory Infections
- Bacteriophages and microbial interactions
- Identification and Quantification in Food
- Plant Pathogenic Bacteria Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Microbial Community Ecology and Physiology
- Spectroscopy and Chemometric Analyses
- Cancer therapeutics and mechanisms
- Tuberculosis Research and Epidemiology
Universidade do Porto
2016-2025
University of Aveiro
2024
Centre for Health Technology and Services Research
2024
Rede de Química e Tecnologia
2014-2023
Cooperativa de Ensino Superior Politécnico e Universitário
2023
Centro Hospitalar do Porto
2016-2018
Hospital de Egas Moniz
2016
Service de Santé des Armées
2016
Hospital Universitario Ramón y Cajal
2006-2015
Instituto Ramón y Cajal de Investigación Sanitaria
2011-2015
Abstract We analyzed 43 CTX-M-15–producing Escherichia coli isolates and 6 plasmids encoding the blaCTX-M-15 gene from Canada, India, Kuwait, France, Switzerland, Portugal, Spain. Most belonged to phylogroups B2 (50%) D (25%). An EC-B2 strain of clonal complex sequence type (ST) 131 was detected in all countries; other corresponded ST28, ST405, ST354, ST695 specific areas. EC-D strains were clonally unrelated but 3 countries ST405. All CTX-M-15 IncFII group with overrepresentation...
The spread of extended-spectrum-beta-lactamase (ESBL)-producing organisms, particularly those harboring the CTX-M-type enzymes, both in hospital and community, is difficult to discontinue due successful mobilization evolution genetic elements ESBL genes coresistance rates these isolates. activities tigecycline against 285 non-clonally related isolates (172 from Escherichia coli, 84 Klebsiella spp., 20 Enterobacter 5 Salmonella 4 Citrobacter spp.) expressing well-characterized ESBLs recovered...
The reliability of Fourier-transform infrared (FT-IR) spectroscopy for
The spread of CTX-M-1-like enzymes in Spain is associated with particular plasmids broad-host-range IncN (blaCTX-M-32, blaCTX-M-1), IncL/M (blaCTX-M-1), and IncA/C2 (blaCTX-M-3) or narrow-host-range IncFII (blaCTX-M-15). identical genetic surroundings blaCTX-M-32 blaCTX-M-1 their locations on related 40-kb indicate the vivo evolution this element.
This study analyzes the diversity of In60, a class 1 integron bearing CR1 and containing bla(CTX-M-9), its association with Tn402, Tn21, classical conjugative plasmids among 45 CTX-M-9-producing clinical strains (41 Escherichia coli strains, 2 Klebsiella pneumoniae Salmonella enterica strain, Enterobacter cloacae strain). Forty-five patients in Spanish tertiary care hospital were studied (1996 to 2003). The In60 Tn402 or mercury resistance transposons investigated by overlapping PCR assays...
Since its first description in 2000, CTX-M-14 has become one of the most widespread extended-spectrum beta-lactamases Spain. In present Escherichia coli multilevel population genetic study involving characterization phylogroups, clones, plasmids, and platforms, 61 isolates from 16 hospitalized patients 40 outpatients healthy volunteers recovered 2000 to 2005 were analyzed. Clonal relatedness (XbaI pulsed-field gel electrophoresis [PFGE] type, phylogenetic group, multilocus sequence type...
Extended-spectrum beta-lactamases (ESBL) constitute a key antibiotic-resistance mechanism affecting Gram-negative bacteria, and also an excellent model for studying evolution in real time. A shift the epidemiology of ESBLs is being observed, which characterized by explosive diversification increase frequency CTX-M-type different settings. This provides unique opportunity protein evolutionary radiation sequential acquisition specific mutations enhancing efficiency fitness concomitantly. The...
KPC-3-producing bacteria are endemic in many countries but only recently became apparent their wide distribution different Portuguese hospitals. The aim of this study is to characterize genetic backgrounds associated with bla KPC-3 among Klebsiella pneumoniae isolates identified on non-hospitalized patients Portugal. Twenty KPC-producing K. between October 2014 and November 2015 three community laboratories were characterized. Isolates mainly from long-term care facilities (n = 11) or...
The rise of antibiotic resistance in the food chain is influenced by use antimicrobial agents, such as antibiotics, metals, and biocides, throughout entire farm-to-fork continuum. Besides, non-clinical reservoirs potentially contribute to transmission critical pathogens multidrug-resistant (MDR) Klebsiella pneumoniae . However, limited knowledge exists about population structure genomic diversity K. circulating conventional poultry production. We conducted a comprehensive characterization...
We describe a hospital-based outbreak caused by multidrug-resistant, Klebsiella pneumoniae carbapenemase 3-producing, mcr-1-positive K. sequence type 45 in Portugal. mcr-1 was located an IncX4 plasmid. Our data highlight the urgent need for systematic surveillance of to support adequate therapeutic choices nosocomial setting.
A Gram-stain-negative strain, A60T, isolated from a water well sample in Portugal, was characterized phenotypically, genotypically and phylogenetically. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A60T belonged to the genus Citrobacter, recN phylogeny revealed one strongly supported clade encompassing 13 other strains public databases, distinct currently recognized species of Citrobacter. Furthermore, multilocus sequence (MLSA) concatenated partial fusA,...
Genomics-based population analysis of multidrug-resistant (MDR) Klebsiella pneumoniae motivated a renewed interest on the capsule as an evolutionary and virulence marker clinically relevant strains. Whole-genome sequencing (WGS)-based approaches have provided great insights into genetic variability capsular locus, but genotypic-biochemical (K)-type correlations are lacking, hindering establishment reliable framework for K-type characterization typing. To fill this gap, we combined molecular,...
Klebsiella pneumoniae sequence type 14 (ST14) and ST15 caused outbreaks of CTX-M-15 and/or carbapenemase producers worldwide, but their phylogeny global dynamics remain unclear. We clarified the evolution K. clonal group (CG14) CG15 by analyzing capsular locus (KL), resistome, virulome, plasmidome public genomes (n = 481) de novo sequences 9) representing main sublineages circulating in Portugal. CG14 evolved independently within 6 subclades defined according to KL accessory genome. The 65)...
To investigate the occurrence and diversity of Ambler class A ESBLs among Enterobacteriaceae from different Portuguese clinical settings over a 2 year period (2002-04).One hundred nine extended-spectrum beta-lactamase (ESBL)-producing isolates five geographically distant health institutions in Portugal were studied. characterized by isoelectric focussing, PCR further sequencing. Antibiotic susceptibility testing, transfer resistance genes clonal determined standard procedures. Plasmid...
ABSTRACT The characterization of a broad representative sample ST131 Escherichia coli isolates from different origins and settings (1991 to 2010) revealed that this clonal group has likely diversified recently the expansion particular variants probably been favored by capture diverse, multidrug-resistant IncFII plasmids (pC15-1a, pEK499, pKF3-140-like). low ability adhere grow as biofilm was detected in study suggests unknown mechanisms for persistence which need be further explored.
The spread of OXA-24/OXA-40 (OXA-24/40)-producing Acinetobacter spp. in the Iberian Peninsula has been strongly influenced by clonal expansion, but role horizontal gene transfer scarcely explored. bla(OXA-24/40)-carrying plasmids and genetic environments were characterized representative (n = 15) species clinical isolates (obtained between 2001 2007) baumannii PCR-based replicon typing, sequencing, hybridization, restriction fragment length polymorphism. Besides identification bla(OXA-24/40)...