A5051904907- Plant nutrient uptake and metabolism
- Wheat and Barley Genetics and Pathology
- Plant Stress Responses and Tolerance
- Ion Transport and Channel Regulation
- Phytase and its Applications
- Plant Disease Resistance and Genetics
- Food composition and properties
- Nitrogen and Sulfur Effects on Brassica
- Seed Germination and Physiology
- Horticultural and Viticultural Research
- Plant responses to water stress
- Membrane-based Ion Separation Techniques
- Plant Genetic and Mutation Studies
- Crop Yield and Soil Fertility
- Plant Micronutrient Interactions and Effects
- Plant Physiology and Cultivation Studies
- Animal Genetics and Reproduction
- Obesity and Health Practices
- Plant pathogens and resistance mechanisms
- Gestational Diabetes Research and Management
- Photosynthetic Processes and Mechanisms
- Tissue Engineering and Regenerative Medicine
- French Urban and Social Studies
- Agricultural pest management studies
- Bioenergy crop production and management
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2016-2024
Génétique, Diversité, Écophysiologie des Céréales
2009-2024
Université Clermont Auvergne
2018-2024
Institut des Sciences des Plantes de Montpellier
2008-2012
Institut Agro Montpellier
2008-2011
Université de Montpellier
2008-2011
Centre National de la Recherche Scientifique
2008-2011
Institut de Recherche pour le Développement
2011
Université d'Angers
2006-2009
Graduate School Experimental Plant Sciences
2006
The water uptake capacity of plant roots (i.e. their hydraulic conductivity, Lp(r)) is determined in large part by aquaporins the plasma membrane intrinsic protein (PIP) subfamily. In present work, we investigated two stimuli, salicylic acid (SA) and salt, because ability to induce an accumulation reactive oxygen species (ROS) inhibition Lp(r) concomitantly Arabidopsis plants. SA was partially counteracted preventing hydrogen peroxide (H(2)O(2)) with exogenous catalase. addition, H(2)O(2)...
Aquaporins form a family of water and solute channel proteins are present in most living organisms. In plants, aquaporins play an important role the regulation root transport response to abiotic stresses. this work, we investigated phosphorylation plasma membrane intrinsic protein (PIP) Arabidopsis thaliana by combination quantitative mass spectrometry cellular biology approaches. A novel phosphoproteomics procedure that involves purification, phosphopeptide enrichment with TiO(2) columns,...
Abstract A proteomic analysis was performed on the heat stable protein fraction of imbibed radicles Medicago truncatula seeds to investigate whether proteins can be identified that are specifically linked desiccation tolerance (DT). Radicles were compared before and after emergence (2.8 mm long) in association with loss DT, reinduction DT by an osmotic treatment. To separate induced treatment from those comparison extended 5 long emerged for which could no longer reinduced, albeit drought...
Abstract To gain insights into the natural variation of root hydraulics and its molecular components, genotypic differences related to water transport plasma membrane intrinsic protein (PIP) aquaporin expression were investigated in 13 accessions Arabidopsis (Arabidopsis thaliana). The hydraulic conductivity excised systems (Lpr) showed a 2-fold among accessions. contribution aquaporins uptake was characterized using as inhibitors mercury, propionic acid, azide. aquaporin-dependent...
Abstract Dolichols are long-chain unsaturated polyisoprenoids with multiple cellular functions, such as serving lipid carriers of sugars used for protein glycosylation, which affects trafficking in the endoplasmic reticulum. The biological functions dolichols plants largely unknown. We isolated an Arabidopsis thaliana mutant, lew1 (for leaf wilting1), that showed a leaf-wilting phenotype under normal growth conditions. LEW1 encoded cis-prenyltransferase, when expressed Escherichia coli...
Late embryogenesis-abundant (LEA) proteins are one of the components involved in desiccation tolerance (DT) by maintaining cellular structures dry state. Among them, MtPM25, a member group 5 is specifically associated with DT Medicago truncatula seeds. Its function unknown and its classification as LEA protein remains elusive. Here, evidence provided that MtPM25 hydrophobic, intrinsically disordered shares characteristics canonical proteins. Screening protective activities testing various...
The concentration and composition of the gliadin glutenin seed storage proteins (SSPs) in wheat flour are most important determinants its end-use value. In cereals, synthesis SSPs is predominantly regulated at transcriptional level by a complex network involving least five cis-elements gene promoters. high-molecular-weight subunits (HMW-GS) encoded two tightly linked genes located on long arms group 1 chromosomes. Here, we sequenced annotated HMW-GS promoters 22 electrophoretic alleles to...
Aquaporins, which facilitate the diffusion of water across biological membranes, are key molecules for regulation transport at cell and organ levels. We recently reported that hydrogen peroxide (H2O2) acts as an intermediate in Arabidopsis root aquaporins response to NaCl salicylic acid (SA).1 Its action involves signaling pathways internalization from surface. The present addendum connects these findings another recent work describes multiple phosphorylations C-terminus expressed plasma...
Summary Wheat grain storage proteins ( GSP s) make up most of the protein content and determine flour end‐use value. The synthesis accumulation s depend highly on nitrogen (N) sulfur (S) availability it is important to understand underlying control mechanisms. Here we studied how einkorn Triticum monococcum ssp. ) proteome responds different amounts N S supply during development. composition at maturity was clearly impacted by nutrition treatments, due early changes in rate filling....
Summary The quality of wheat grain is mainly determined by the quantity and composition its storage proteins ( GSP s). Grain consist low‐ high‐molecular‐weight glutenins LMW ‐ GS HMW , respectively) gliadins. synthesis these essentially regulated at transcriptional level availability nitrogen sulfur. regulation network has been extensively studied in barley where BLZ 1 2, members basic leucine zipper bZIP ) family, activate hordeins. To date, wheat, only ortholog Storage Protein Activator...
Grain storage proteins (GSPs) quantity and composition determine the end-use value of wheat flour. GSPs consists low-molecular-weight glutenins (LMW-GS), high-molecular-weight (HMW-GS) gliadins. GSP gene expression is controlled by a complex network DNA-protein protein-protein interactions, which coordinate tissue-specific protein during grain development. The regulatory has been most extensively studied in barley, particularly two transcription factors (TFs) DNA binding with One Finger...
Wheat grain end-use value is determined by complex molecular interactions that occur during development, including those in the cell nucleus. However, our knowledge of how nuclear proteome changes development limited. Here, we analyzed proteins developing wheat grains collected cellularization, effective grain-filling, and maturation phases respectively. Nuclear were extracted separated two-dimensional gel electrophoresis. Image analysis revealed 371 299 reproducible spots gels with first...