A5069145942- Prostate Cancer Treatment and Research
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- PI3K/AKT/mTOR signaling in cancer
- Wnt/β-catenin signaling in development and cancer
- Cancer-related gene regulation
- Estrogen and related hormone effects
- Immune Cell Function and Interaction
- Hippo pathway signaling and YAP/TAZ
- Immunotherapy and Immune Responses
- Reproductive System and Pregnancy
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- T-cell and B-cell Immunology
- Ubiquitin and proteasome pathways
- Hormonal and reproductive studies
- Heat shock proteins research
- Cancer, Lipids, and Metabolism
- Protein Hydrolysis and Bioactive Peptides
- Eicosanoids and Hypertension Pharmacology
- HIV Research and Treatment
- Genital Health and Disease
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Reproductive Physiology in Livestock
- Cancer-related molecular mechanisms research
Nanjing Agricultural University
2020-2025
Chinese PLA General Hospital
2025
Anhui Medical University
2025
Harvard University
2012-2024
Beth Israel Deaconess Medical Center
2012-2024
Nankai University
2009-2024
Soochow University
2010-2024
First Affiliated Hospital of Soochow University
2010-2024
National Clinical Research Center for Digestive Diseases
2024
First Affiliated Hospital Zhejiang University
2023-2024
Testosterone supplementation in men decreases fat mass; however, the mechanisms by which it inhibits mass are unknown. We hypothesized that testosterone adipogenic differentiation of preadipocytes activation androgen receptor (AR)/beta-catenin interaction and subsequent translocation this complex to nucleus thereby bypassing canonical Wnt signaling. tested hypothesis 3T3-L1 cells differentiate form medium. found these express AR dihydrotestosterone dose-dependently inhibited as analyzed Oil...
Fusion of the androgen receptor-regulated (AR-regulated) TMPRSS2 gene with ERG in prostate cancer (PCa) causes androgen-stimulated overexpression ERG, an ETS transcription factor, but critical downstream effectors ERG-mediating PCa development remain to be established. Expression SOX9 factor correlated TMPRSS2:ERG fusion 3 independent cohorts, and ERG-dependent expression was confirmed by RNAi fusion-positive VCaP cell line. has been shown mediate ductal morphogenesis fetal maintain...
Abstract Metastatic castration-resistant prostate cancer is typically lethal, exhibiting intrinsic or acquired resistance to second-generation androgen-targeting therapies and minimal response immune checkpoint inhibitors 1 . Cellular programs driving in both cells remain poorly understood. We present single-cell transcriptomes from 14 patients with advanced cancer, spanning all common metastatic sites. Irrespective of treatment exposure, adenocarcinoma pervasively coexpressed multiple...
Accumulating evidence suggests that the matrix (MA) protein of retroviruses plays a key role in virus assembly by directing intracellular transport and membrane association Gag polyprotein. In this report, we show MA human immunodeficiency type 1 is also critical for incorporation viral Env proteins into mature virions. Several deletions introduced domain (p17) polyprotein did not greatly affect synthesis processing or formation Analysis revealed normal levels Pol these mutant virions, but...
The matrix domain of human immunodeficiency virus type 1 Gag polyprotein was studied for its role in assembly. Deletion and substitution mutations caused a dramatic reduction production. Mutant polyproteins were myristoylated had high affinity membrane association. Immunofluorescence staining revealed large accumulation mutant precursors the cytoplasm, while wild-type proteins primarily associated with cell surface membrane. These results suggest defect intracellular transport precursors....
Androgen receptors (ARs) are phosphorylated at multiple sites in response to ligand binding, but the kinases mediating AR phosphorylation and importance of these function have not been established. Here we show that cyclin-dependent kinase 1 (Cdk1) mediates Ser-81 increases protein expression, Cdk1 inhibitors decrease phosphorylation, transcriptional activity prostate cancer (PCa) cells. The decline expression mediated by Cdk inhibitor roscovitine was prevented proteosome inhibitors,...
The transcription factor SOX9 is critical for prostate development, and dysregulation of implicated in cancer (PCa). However, the SOX9-dependent genes pathways involved both normal neoplastic epithelium are largely unknown. Here, we performed ChIP sequencing analysis transcriptome profiling PCa cells determined that positively regulates multiple WNT pathway genes, including those encoding receptors (frizzled [FZD] lipoprotein receptor-related protein [LRP] family members) downstream...
Abstract SOX9 is a transcription factor that plays critical role in the development of multiple tissues. We previously reported normal human adult prostate was restricted to basal epithelium. also expressed subset cancer (PCa) cells and increased relapsed hormone-refractory PCa. Moreover, expression PCa cell lines enhanced tumor proliferation β-catenin regulated. Here we report additional vivo results showing highly during fetal by epithelial expanding into mesenchyme, suggesting it may...
Evodiamine (EVO), an extraction from the traditional Chinese medicine Evodia rutaecarpa, has been reported to possess anti-inflammatory, anti-tumor and other pharmacological activities. However, effectiveness of EVO relieve dextran sodium sulfate (DSS)-induced ulcerative colitis (UC) not evaluated. In this study, protective effects mechanisms on DSS-induced UC mice were investigated. The results indicated that treatment with ameliorated body weight loss, disease activity index (DAI), colon...
PIK3CA mutations occur in ∼8% of cancers, including ∼40% HR-positive breast where the PI3K-alpha (PI3Kα)-selective inhibitor alpelisib is FDA approved combination with fulvestrant. Although prior studies have identified resistance mechanisms, such as PTEN loss, clinically acquired to PI3Kα inhibitors remains poorly understood. Through serial liquid biopsies and rapid autopsies 39 patients advanced cancer developing inhibitors, we observe that 50% acquire genomic alterations within PI3K...
Noncanonical Wnt signaling by WNT5a has oncogenic and tumor suppressive activities, but downstream pathways mediating these specific effects remain to be fully established. In a subset of prostate cancer organoid culture xenograft models, inhibition synthesis stimulated growth, whereas or mimetic peptide (Foxy5) markedly suppressed growth. caused ROR2-dependent decrease in YAP1 activity, which was associated with increased phosphorylation MST1/2, LATS1, MOB1, YAP1, indicating Hippo pathway...
In-frame stop codons were introduced into the coding region of human immunodeficiency virus type 1 (HIV-1) transmembrane protein (gp41). Truncation 147 amino acids from carboxyl terminus gp41 (TM709) significantly decreased stability and cell surface expression viral Env proteins, while truncation 104 (TM752) did not. 43 or more generated mutant viruses which noninfectious in several CD4+ T lymphoid lines fresh peripheral blood mononuclear cells. Analysis virions revealed reduced...
SOX9 is a member of the SOX [Sry-related high-mobility group (HMG) box] family HMG DNA-binding domain transcription factors and required for development differentiation multiple cell lineages. This report shows that basal epithelial cells express in normal prostate, with no detectable expression luminal cells. In contrast, expressed primary prostate cancers vivo, at higher frequency recurrent cancer lines (LNCaP, CWR22, PC3, DU145). message protein levels were increased by treatment glycogen...
The vpr gene of human immunodeficiency virus type 1 (HIV-1) is one the seven accessory genes that are believed to have roles in replication cycle. We report here detection a 13 kD protein sucrose gradient-purified HIV-1. This was not detected cells infected with having truncated lacks potential encode for 26 C-terminal amino acid residues. These findings raise possibility virion-associated proteins may be involved early life cycle HIV-1 and suggest region essential its expression.
Loss of PTEN is a common event in many cancers and leads to hyperactivation the PI3K-AKT signaling pathway. The mechanisms by which AKT isoforms mediate phenotypes associated with inactivation cancer have not been defined. Here, we show that AKT2 exclusively required for PTEN-deficient prostate tumor spheroid maintenance, whereas AKT1 dispensable. shRNA silencing but promotes regression xenografts. Mechanistically, upregulates p21 proapoptotic protein BAX downregulates insulin-like growth...
Whether the human tumor virus, Epstein-Barr Virus (EBV), promotes breast cancer remains controversial and a potential mechanism has remained elusive. Here we show that EBV can infect primary mammary epithelial cells (MECs) express receptor CD21. infection leads to expansion of early MEC progenitor with stem cell phenotype, activates MET signaling enforces differentiation block. When MECs were implanted as xenografts, cooperated activated Ras accelerated formation cancer. Infection in...
This study investigated interactions between SRY, the Y chromosome encoded male sex determining factor, and androgen receptor (AR). Coexpression of AR SRY caused marked repression transcriptional activity on a series androgen-responsive reporter genes. Mammalian one- two-hybrid experiments demonstrated an AR-SRY interaction mediated by DNA binding domain. Precipitations with glutathione S-transferase fusion proteins indicated that were direct domain high mobility group box Transient...