A5069105512- 3D Printing in Biomedical Research
- Viral Infectious Diseases and Gene Expression in Insects
- Electrospun Nanofibers in Biomedical Applications
- Microfluidic and Bio-sensing Technologies
- Nanoparticles: synthesis and applications
- Surgical Sutures and Adhesives
- Wastewater Treatment and Nitrogen Removal
- Pancreatic function and diabetes
- Photoacoustic and Ultrasonic Imaging
- Bone Tissue Engineering Materials
- Polymer Surface Interaction Studies
- Inflammatory mediators and NSAID effects
- Tissue Engineering and Regenerative Medicine
- Nanocomposite Films for Food Packaging
- Neuroendocrine Tumor Research Advances
- Graphene and Nanomaterials Applications
- Cancer Cells and Metastasis
- Nanoplatforms for cancer theranostics
- Microbial Metabolic Engineering and Bioproduction
- Wound Healing and Treatments
- Gold and Silver Nanoparticles Synthesis and Applications
- Essential Oils and Antimicrobial Activity
- Cancer, Hypoxia, and Metabolism
Institute of Chemical Technology
2018-2021
National Chemical Laboratory
2011-2017
Council of Scientific and Industrial Research
2011
Hislop College
2011
In this study, we have combined the wound-healing properties of two biodegradable polymers, viz., starch and gelatin, reinforced their mechanical strength through cross-linking. Further, scaffolds polymer combination were used to support an organotypic culture human skin for wound healing. Human dermal fibroblasts (HDFs) epidermal keratinocytes (HEKs) isolated seeded on days 1 7, respectively. The scaffold was then air-lifted develop a stratified layer. Hematoxylin eosin (H&E) staining...
Abstract Though cell culture‐based protein production processes are rarely carried out under batch mode of operation, line and initial process development operations usually in due to simplicity operation widely used scale down platforms like shake flasks. Nutrient feeding, if performed, is achieved by bolus addition concentrated feed solution at different intervals, which leads large transient increases nutrient concentrations. One negative consequence increased waste metabolite production....
pH in animal cell cultures decreases due to production of metabolites like lactate. control via measurement and base addition is not easily possible small-scale culture formats tissue-culture flasks shake flasks. A hydrogel-based system reported for situ maintenance without such formats, demonstrated maintain between 6.8 7.2 a suspension CHO line CD medium 7.3 7.5 adherent A549 cells DMEM:F12 containing 10% FBS. This maintenance, along with our previous report hydrogels controlled nutrient...
Bone regeneration and repair are complex processes with the potential of added complications, like delayed repair, fracture non-union, post-surgical infections. These conditions remain a challenge globally, pressurizing economy patients suffering from these conditions. Applications nanotechnology (NBT) in field medicine have provided medium for several approaches to support global challenges. Tissue engineering is one such that has been on rise last three decades through utilization NBT...
Inorganic phosphate (P i ) is an essential ion involved in diverse cellular processes including metabolism. Changes metabolism upon long term adaptation to P limitation have been reported E. coli . Given the role of , may also result metabolic changes animal cells. In this study, we adapted CHO cells producing recombinant IgG limiting conditions for 75 days. Not surprisingly, showed better survival under limitation. Here, report finding that such growth characteristics compared control batch...
Abstract 3D cell cultures are regarded as a better and more relevant approach for screening drugs therapeutics, particularly due to their likeness with the in vivo conditions. Spheroids offer an intermediate platform between vitro models, conducting tumor‐based investigations. In this study, simple setup was developed consistent generation of lung co‐culture spheroids, which were using cancer lines A549, NCI H460, fibroblast cells WI‐38. The potential these spheroids evaluating toxicity...
Microcarrier technology has emerged as one of the promising platforms for large scale production anchorage dependent cells. Cells grown on these carriers have been utilized applications ranging from tissue engineering to vaccine production. Porous chitosan microcarriers (CMC), having diameter in range 250 – 300 µm and suitability cell adhesion proliferation, were prepared evaluated this study. CMC cross-linked by ionic gelation method, using sodium tripolyphosphate, freeze dried obtain...