A5063902045- Urinary Tract Infections Management
- Escherichia coli research studies
- Antimicrobial Resistance in Staphylococcus
- Antibiotic Resistance in Bacteria
- Urinary Bladder and Prostate Research
- Bacterial Identification and Susceptibility Testing
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Gut microbiota and health
- Pelvic floor disorders treatments
- Streptococcal Infections and Treatments
- Vitamin D Research Studies
- Antimicrobial Peptides and Activities
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Microbial infections and disease research
- Bladder and Urothelial Cancer Treatments
- Yersinia bacterium, plague, ectoparasites research
- Probiotics and Fermented Foods
- Immune Response and Inflammation
- Menstrual Health and Disorders
- Cell Image Analysis Techniques
- Biochemical effects in animals
- Biochemical and Structural Characterization
- Clostridium difficile and Clostridium perfringens research
Karolinska Institutet
2009-2022
Institut für Tier-, Natur- und Umweltethik
2022
Karolinska University Hospital
2009-2018
University of Veterinary Medicine Hannover, Foundation
2017
Faktor 10 – Institut für nachhaltiges Wirtschaften gemeinnützige
2006-2007
Bacterial growth in multicellular communities, or biofilms, offers many potential advantages over single-cell growth, including resistance to antimicrobial factors. Here we describe the interaction between biofilm-promoting components curli fimbriae and cellulose of uropathogenic E. coli endogenous defense urinary tract. We also demonstrate impact this interplay on pathogenesis tract infections. Our results suggest that exhibit differential complementary functions. Both these biofilm were...
Objectives: The aim of this study was to analyse coagulase-negative staphylococci (CoNS) for their resistance antimicrobial agents approved the control pathogens involved in bovine mastitis, with particular reference macrolide and/or lincosamide (ML) and genes involved. Methods: A total 298 CoNS collected between 2003 2005 Germany from cases subclinical mastitis dairy cows were identified species level investigated MICs by broth microdilution. ML-resistant isolates subjected plasmid...
The urinary tract is frequently being exposed to potential pathogens and rapid defence mechanisms are therefore needed. Cathelicidin, a human antimicrobial peptide expressed secreted by bladder epithelial cells protects the from infection. Here we show that vitamin D can induce cathelicidin in bladder. We analyzed tissue postmenopausal women for expression of cathelicidin, before after three-month period supplementation with 25-hydroxyvitamin D3 (25D3). Cell culture experiments were...
Estrogen supports urothelial defense against infection by increasing the expression of antimicrobial peptides and protecting epithelial integrity.
Summary Major long-term complications in patients with diabetes are related to oxidative stress, caused by the hyperglycaemia characteristic for mellitus. The anti-oxidant coenzyme Q10 (CoQ10) has therefore been proposed as a beneficial supplement treatment. Apart from its anti-oxidative function, CoQ10 appears modulate immune functions largely unknown mechanisms. aim of this study was investigate effect on antimicrobial peptides and natural killer (NK) cells, both innate components...
ObjectivesTo date, very little is known about lincosamide resistance plasmids in staphylococci with only a single lnu(A)-carrying staphylococcal plasmid having been sequenced completely. The aim of this study was to characterize small isolated from bovine coagulase-negative (CoNS).
Vitamin D stimulates production of the endogenous antimicrobial peptides cathelicidin and β-defensin-2, which are expressed in urinary tract. We investigated vitamin status levels β-defensin-2 their association with tract infection (UTI).The study included 120 children under three years age: 76 UTIs 44 otherwise healthy congenital hydronephrosis. Serum 25-hydroxycholecalciferol were measured by direct competitive electro-chemiluminescence immunoassay, plasma concentrations analysed...
We compared the newly approved BacT/Alert Virtuo blood culture system to 3D using 115 clinical bacterial and fungal isolates in 784 simulated bottles. The time detection was reduced by roughly 20% (P< 0.0001) while rate did not differ.
The human leukemia cell line HL-60 is considered an alternative culture model to study neutrophil differentiation and migration. aim of this was characterize the suitability cells differentiated neutrophil-like (nHL-60) as substitute for blood-derived neutrophils investigate interaction with Staphylococcus aureus.For purpose, antimicrobial activity, bacterial uptake, production reactive oxygen species release extracellular traps (NETs) by nHL-60 were analyzed compared primary using aureus...
Neutrophil extracellular trap (NET) formation is described as a tool of the innate host defence to fight against invading pathogens. Fibre-like DNA structures associated with proteins such histones, cell-specific enzymes and antimicrobial peptides are released, thereby entrapping It has been reported that several bacteria able degrade NETs by nucleases thus evade NET-mediated entrapment. Here we studied ability three different Yersinia serotypes induce NETs. We found common enterocolitica...
Agar plate-based biofilm of enterobacteria like Escherichia coli is characterized by expression the extracellular matrix components amyloid curli and cellulose exopolysaccharide, which can be visually enhanced upon addition dye Congo Red, resulting in a red, dry, rough (rdar) colony morphology. Expression rdar morphotype depends on transcriptional regulator CsgD occurs predominantly at ambient temperature model strains. In contrast, commensal pathogenic isolates frequently express...
Vitamin D deficiency is a common health problem with consequences not limited to bone and calcium hemostasis. Low levels have also been linked tuberculosis other respiratory infections as well autoimmune diseases. We previously shown that supplementation vitamin can induce the antimicrobial peptide cathelicidin during ex vivo infection of human urinary bladder. In rodents, however, expression therefore this D-related effect fighting bacterial invasion relevant. To determine if had further...
The clinical demand on rapid microbiological diagnostic is constantly increasing. PCR coupled to electrospray ionization-mass spectrometry, PCR/ESI-MS, offers detection and identification of over 750 bacteria Candida species directly from specimens within 6 hours. In this study, we investigated the performance IRIDICA BAC LRT Assay for bacterial pathogens in 121 bronchoalveolar lavage (BAL) samples that were received consecutively at our laboratory BAL culture. Commensal or pathogenic...
Objectives: The complete nucleotide sequence of the 6913 bp plasmid pSTE2 from Staphylococcus lentus, which mediates inducible resistance to tetracyclines, macrolides and lincosamides, was determined. analysed for potential reading frames structural features gain insight into its development ancestor plasmids. Methods: Plasmid transformed aureus RN4220. Suitable restriction fragments were cloned E. coli vectors sequenced. In vitro susceptibility testing performed confirm phenotype mediated...