A5044282058- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Respiratory viral infections research
- Cytomegalovirus and herpesvirus research
- Animal Virus Infections Studies
- COVID-19 epidemiological studies
- RNA and protein synthesis mechanisms
- Monoclonal and Polyclonal Antibodies Research
- Tuberculosis Research and Epidemiology
- SARS-CoV-2 detection and testing
- Bacterial Identification and Susceptibility Testing
- Viral Infections and Vectors
- Animal Disease Management and Epidemiology
- Infectious Diseases and Tuberculosis
- Antimicrobial Peptides and Activities
- Mycobacterium research and diagnosis
- Menstrual Health and Disorders
- Urinary Tract Infections Management
- Prostate Cancer Diagnosis and Treatment
- COVID-19 Pandemic Impacts
- Vector-borne infectious diseases
- T-cell and B-cell Immunology
- RNA modifications and cancer
Karolinska University Hospital
2010-2024
Karolinska Institutet
2010-2024
A novel coronavirus (SARS-CoV-2) first detected in Wuhan, China, has spread rapidly since December 2019, causing more than 100,000 confirmed infections and 4000 fatalities (as of 10 March 2020). The outbreak been declared a pandemic by the WHO on Mar 11, 2020. Here, we explore how seasonal variation transmissibility could modulate SARS-CoV-2 pandemic. Data from routine diagnostics show strong consistent four endemic coronaviruses (229E, HKU1, NL63, OC43) parameterise our model for using...
A novel coronavirus (SARS-CoV-2) first detected in Wuhan, China, has spread rapidly since December 2019, causing more than 80,000 confirmed infections and 2,700 fatalities (as of Feb 27, 2020). Imported cases transmission clusters various sizes have been reported globally suggesting a pandemic is likely. Here, we explore how seasonal variation transmissibility could modulate SARS-CoV-2 pandemic. Data from routine diagnostics show strong consistent the four endemic coronaviruses (229E, HKU1,...
Pre-existing SARS-CoV-2-reactive T cells have been identified in SARS-CoV-2-unexposed individuals, potentially modulating COVID-19 and vaccination outcomes. Here, we provide evidence that functional cross-reactive memory CD4 + cell immunity against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is established early childhood, mirroring seroconversion with seasonal human OC43. Humoral cellular immune responses OC43 SARS-CoV-2 were assessed children (paired samples at age two...
Descendants of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant now account for almost all SARS-CoV-2 infections. The and its sublineages have spike glycoproteins that are highly diverged from pandemic founder first-generation vaccine strain, resulting in significant evasion monoclonal antibody therapeutics vaccines. Understanding how commonly elicited antibodies can broaden to cross-neutralize escape variants is crucial. We isolate IGHV3-53, using "public"...
The urinary tract is frequently being exposed to potential pathogens and rapid defence mechanisms are therefore needed. Cathelicidin, a human antimicrobial peptide expressed secreted by bladder epithelial cells protects the from infection. Here we show that vitamin D can induce cathelicidin in bladder. We analyzed tissue postmenopausal women for expression of cathelicidin, before after three-month period supplementation with 25-hydroxyvitamin D3 (25D3). Cell culture experiments were...
We report a rapid increase in enterovirus D68 (EV-D68) infections, with 139 cases reported from eight European countries between 31 July and 14 October 2021. This upsurge is line the seasonality of EV-D68 was presumably stimulated by widespread reopening after COVID-19 lockdown. Most were identified September, but more are to be expected coming months. Reinforcement clinical awareness, diagnostic capacities surveillance urgently needed Europe.
Worldwide outbreaks of enterovirus D68 (EV-D68) in 2014 and 2016 have caused serious respiratory neurological disease. We collected samples from several European countries during the 2018 outbreak determined 53 near full-length genome ('whole genome') sequences. These sequences were combined with 718 whole 1,987 VP1-gene publicly available In 2018, circulating strains clustered into multiple subgroups B3 A2 subclades, different phylogenetic origins. Clusters subclade emerged primarily US...
The SARS-CoV-2 Omicron 1 Variant of Concern (B.1.1.529) has spread rapidly in many countries. With a spike that is highly diverged from the pandemic founder, it escapes most available monoclonal antibody therapeutics 2,3 and erodes vaccine protection 4 . A public class IGHV3-53-using neutralizing antibodies 5,6 typically fails to neutralize variants carrying mutations receptor-binding motif 7–11 , including Omicron. As this are likely elicited people following infection or vaccination, their...
Abstract Enterovirus D68 (EV-D68) infections are associated with severe respiratory disease and acute flaccid myelitis (AFM). The European Non-Polio Network (ENPEN) aimed to investigate the epidemiological genetic characteristics of EV-D68 its clinical impact during fall-winter season 2021–2022. From 19 countries, 58 institutes reported 10 481 (6.8%) EV-positive samples which 1004 (9.6%) were identified as (including 852 samples). Clinical data for 969 cases; 78.9% in children (0–5 years);...
We report an enterovirus D68 (EV-D68) outbreak in Stockholm Sweden 2016. Between 22 August and 25 September EV-D68 was detected 74/495 respiratory samples analysed at the Karolinska University Hospital. During peak week, 30/91 (33%) were positive. Viral protein (VP)P4/VP2 sequencing revealed that cases caused by B3 lineage strains. Forty-four (59%) EV-D68-positive patients children aged ≤ 5 years. Ten had severe or neurological symptoms one died.
An understanding of differences in clinical phenotypes and outcomes COVID-19 compared with other respiratory viral infections is important to optimise the management patients plan healthcare. Herein we sought investigate such positive for SARS-CoV-2 influenza, syncytial virus (RSV) viruses. We performed a retrospective cohort study hospitalised adults children (≤15 years) who tested SARS-CoV-2, influenza A/B, RSV, rhinovirus, enterovirus, parainfluenza viruses, metapneumovirus, seasonal...
Enterovirus D68 (EV-D68) was detected in 93 patients from five European countries between 1 January 2019 and 15 2020, a season with expected low circulation. Patients were primarily children (n = 67, median age: 4 years), 59 required hospitalisation had severe neurologic manifestations. Phylogenetic analysis revealed two clusters the B3 subclade A2/D. This circulation of EV-D68 associated neurological manifestations stresses importance surveillance diagnostics beyond peak years.
In 2018, an upsurge in echovirus 30 (E30) infections was reported Europe. We conducted a large-scale epidemiologic and evolutionary study of 1,329 E30 strains collected 22 countries Europe during 2016-2018. Most cases affected persons 0-4 years age (29%) 25-34 (27%). Sequences were divided into 6 genetic clades (G1-G6). (53%) sequences belonged to G1, followed by G6 (23%), G2 (17%), G4 (4%), G3 (0.3%), G5 (0.2%). Each clade encompassed unique individual recombinant forms; G1 displayed >2...
Bloodstream infection following a transrectal prostate biopsy is well-known and feared complication. Previous studies have shown an increase in multi-resistant bacterial infections as consequence of higher usage antibiotics investigated populations. Our aim was to analyze resistance patterns positive blood cultures, after biopsies Stockholm, Sweden, where the use has been low decreasing during last 10 years.From three pathology laboratories reports examinations were retrieved (n = 56,076)...
Worldwide outbreaks of enterovirus D68 (EV-D68) in 2014 and 2016 have caused serious respiratory neurological disease. To investigate diversity, spread, evolution EV-D68 we performed near full-length deep sequencing fifty-four samples obtained Sweden during the outbreaks. In most samples, intrapatient variability was low dominated by rare synonymous variants, but three patients showed evidence dual infections with distinct variants from same subclade. Interpatient a very strong temporal...
Quantiferon-TB Gold Plus (QFT-Plus) is an interferon gamma release assay used to diagnose latent tuberculosis (LTB). A borderline range (0.20 0.99 IU/ml) around the cutoff (0.35 has been suggested for earlier QFT version.
In 2014, an outbreak of enterovirus D68 (EV-D68) was observed in North America, with cases severe respiratory illness and a possible etiological link to acute flaccid paralysis. EV-D68 has also been reported from European countries, but no data Sweden are available. This study investigated specimens collected during July-October 2014 30 Swedish children aged 0-9 years who were positive for and/or rhinovirus routine clinical PCR. Seven samples typed as by VP4/VP2 sequencing. Two genetically...
Abstract Polymerase chain reaction (PCR) detection has become the gold standard for diagnosis and typing of enterovirus (EV) human parechovirus (HPeV) infections. Its effectiveness depends critically on using appropriate sample types high assay sensitivity as viral loads in cerebrospinal fluid samples from meningitis sepsis clinical presentation can be extremely low. This study evaluated specificity currently used commercial in‐house diagnostic assays. Accurately quantified RNA transcript...
The purpose of this study was to compare the Quantiferon-TB Gold Plus test analysed with chemiluminescence immunoassay (CLIA) (EIA). One hundred and twenty-five clinical specimens submitted Karolinska University Hospital for analysis were used analyse agreement CLIA EIA assays. imprecision on determined by pooled samples antigen tubes (TB1 TB2), a reference material from National Institute Biological Standards Control (NIBSC 82/587) two controls test. Recovery TB1 TB2 NIBSC 82/587....
Tick-borne encephalitis (TBE) is an emerging infection causing CNS of various severity. Good knowledge the incidence in population and defined risk areas important communication vaccination recommendations. The aim this study was to investigate potential underreporting by retrospectively diagnose TBE among patients with viral infections unknown etiology a region for TBE, define variables associated performed serology at time infection. Epidemiological data microbiological diagnostics cases...
Background Worldwide outbreaks of enterovirus D68 (EV-D68) in 2014 and 2016 have caused serious respiratory neurological disease. Methods We collected samples from several European countries during the 2018 out-break determined 53 near full-length genome (‘whole genome’) sequences. These sequences were combined with 718 whole 1,987 VP1-gene publicly available Findings In 2018, circulating strains clustered into multiple subgroups B3 A2 subclades, different phylogenetic origins. Clusters...
Background Despite the unprecedented measures implemented globally in early 2020 to prevent spread of SARS-CoV-2, Sweden, as many other countries, experienced a severe first wave during COVID-19 pandemic. Aim We investigated introduction and SARS-CoV-2 into Sweden. Methods analysed stored respiratory specimens (n = 1,979), sampled 7 February–2 April 2020, by PCR for sequenced PCR-positive specimens. Sequences generated from newly detected cases positive February–June 954) were combined with...