A5085440942- Advanced NMR Techniques and Applications
- Atomic and Subatomic Physics Research
- NMR spectroscopy and applications
- Diverse Educational Innovations Studies
- RNA Research and Splicing
- Superconducting Materials and Applications
- Electron Spin Resonance Studies
- Solid-state spectroscopy and crystallography
- Amyotrophic Lateral Sclerosis Research
- Education Systems and Policy
- Mycotoxins in Agriculture and Food
- Scientific Research and Discoveries
- Advancements in Battery Materials
- Plant Pathogens and Fungal Diseases
- Electronic Health Records Systems
- Advanced biosensing and bioanalysis techniques
- Chronic Disease Management Strategies
- Magnetic and Electromagnetic Effects
- Tree Root and Stability Studies
- Wheat and Barley Genetics and Pathology
- HIV Research and Treatment
- Botany and Plant Ecology Studies
- Artificial Intelligence in Healthcare
- Data Quality and Management
- Quantum, superfluid, helium dynamics
ETH Zurich
2020-2023
Washington University in St. Louis
2018-2020
Judson University
2018-2019
University of Arizona
2015
Robert Wood Johnson Foundation
2015
Agency for Healthcare Research and Quality
2015
United States Department of Agriculture
2010
Aspergillus flavus has two morphotypes, the S strain and L strain, that differ in aflatoxin-producing ability other characteristics. Fungal communities on maize dominated by of A. have repeatedly been associated with acute aflatoxin poisonings Kenya, where management tools to reduce levels are needed urgently. isolates (n = 290) originating from produced Kenya belonging morphotype were tested for potential. A total 96 atoxigenic was identified four provinces sampled. The placed into 53...
The Department of Veterans Affairs Healthcare Administration (VHA) is supported by one the largest integrated health care information systems in United States. VHA's Corporate Data Warehouse (CDW) was developed 2006 to accommodate massive amounts data being generated from more than 20 years use and streamline process knowledge discovery application. This article describes developments research associated with transition into world Big analytics through CDW utilization. majority studies...
Spherical rotors can be used for magic angle spinning nuclear magnetic resonance.
Dynamic nuclear polarization (DNP) is used to improve the inherently poor sensitivity of magnetic resonance spectroscopy by transferring spin from electrons nuclei. However, DNP radicals within sample can have detrimental effects on spins close polarizing agent. Chirped microwave pulses and electron decoupling (eDEC) attenuate these in model systems, but this approach yet be applied intact cells or cellular lysates. Herein, we demonstrate for first time exceptionally fast 1H T1DNP times just...
We demonstrate for the first time in-cell dynamic nuclear polarization (DNP) in conjunction with flow cytometry sorting to address cellular heterogeneity of samples. Utilizing a green fluorescent protein (GFP) reporter HIV reactivation, we correlate increased 15N resonance intensity cytokine-driven reactivation human cell line model latency. As few as 10% GFP+ cells could be detected by DNP magnetic (NMR). The inclusion cytometric prior analysis DNP-NMR further boosted signal detection...
Spherical rotors are a new paradigm in magic angle spinning (MAS) solid-state nuclear magnetic resonance (NMR).The simple geometry makes smaller diameter spheres and their utilization within narrow-bore NMR probes feasible.Here we report 68 kHz frequency of 2 mm using helium gas outside the magnet demonstrate use KMnO 4 to adjust at 59.3 for MAS NMR.We observe third-order sidebands 55 Mn spectrum clearly showing kHz, with KBr nearly no first-order similar frequency.The stability was ±0.5%...
Precise alignment of the sample's spinning axis is vital for many magic angle solid state NMR experiments. Spherical rotors are a new paradigm NMR, having been demonstrated to spin stably with little risk rotor crash, but like cylindrical rotors, they have previously only utilized mechanical adjustment method set rotation angle. Here we show that by using second gas aperture within stator, spherical rotor's may be precisely pitched about without or motion stator. The 2H MAS sideband...
Abstract. The use of spherical rotors for magic angle spinning offers a number advantages, including improved sample exchange, efficient microwave coupling dynamic nuclear polarization magnetic resonance (NMR) experiments, and, most significantly, high frequency and stable with minimal risk rotor crash. Here we demonstrate the simple retrofitting commercial NMR probe MAS spheres solid-state NMR. We analyze series turbine groove geometries to investigate importance surface performance. Of...
Cryogenic magic angle spinning (MAS) is a standard technique utilized for dynamic nuclear polarization (DNP) in solid-state magnetic resonance (NMR). Here we describe the optimization and implementation of stator cryogenic MAS with 9.5 mm diameter spherical rotors, allowing DNP experiments on large sample volumes. Designs rotor build recent advancements spheres take step further to incorporate insert eject temperature-independent stability ±1 Hz. At field 7 T at 2.0 kHz temperature 105-107...
RNA recognition motif-type RNA-binding domain containing proteins (RBDPs) participate in metabolism including regulating mRNA stability, nuclear-cytoplasmic shuttling, and splicing. Rbm45 is an RBDP first cloned from rat brain expressed spatiotemporally during neural development. More recently, RBM45 has been associated with pathological aggregates the human neurological disorders amyotrophic lateral sclerosis, frontotemporal lobar degeneration, Alzheimer’s. developmental protein musashi-1...
Summary RNA recognition motif-type RNA-binding domain containing proteins (RBDPs) participate in metabolism including regulating mRNA stability, nuclear-cytoplasmic shuttling, and splicing. Rbm45 is an RBDP first cloned from rat brain expressed spatiotemporally during neural development. More recently, RBM45 has been associated with pathological aggregates the human neurological disorders amyotrophic lateral sclerosis, frontotemporal lobar degeneration, Alzheimer’s. developmental protein...
Abstract. Cryogenic magic-angle spinning (MAS) is a standard technique utilized for Dynamic Nuclear Polarization (DNP) in solid state nuclear magnetic resonance (NMR). Here we describe the optimization and implementation of stator cryogenic MAS with spherical rotors, allowing DNP experiments on large sample volumes. Designs rotor build recent advancements spheres, take step further to incorporate sample-insert/eject temperature-independent stability +/- 1 Hz. At field 7 T at 2.0 kHz...
Abstract. The use of spherical rotors for magic angle spinning offers a number advantages including improved sample exchange, efficient microwave coupling dynamic nuclear polarization magnetic resonance (NMR) experiments and, most significantly, high frequency and stable with minimal risk rotor crash. Here we demonstrate the sim- ple retrofitting commercial NMR probe MAS spheres solid-state NMR. We analyze series turbine groove geometries to investigate importance surface on performance. Of...