A5050477156- Advanced NMR Techniques and Applications
- NMR spectroscopy and applications
- Physics of Superconductivity and Magnetism
- Atomic and Subatomic Physics Research
- Superconducting Materials and Applications
- Electron Spin Resonance Studies
- Solid-state spectroscopy and crystallography
- Particle accelerators and beam dynamics
- Advanced MRI Techniques and Applications
- Gyrotron and Vacuum Electronics Research
- Quantum, superfluid, helium dynamics
- Particle Accelerators and Free-Electron Lasers
- DNA and Nucleic Acid Chemistry
- Magnetic and transport properties of perovskites and related materials
- Advanced Chemical Sensor Technologies
- Identification and Quantification in Food
- Frequency Control in Power Systems
- Biochemical Analysis and Sensing Techniques
- Astronomical Observations and Instrumentation
- Advanced biosensing and bioanalysis techniques
- Adaptive optics and wavefront sensing
- ZnO doping and properties
- RNA Research and Splicing
- HIV Research and Treatment
- Astronomy and Astrophysical Research
ETH Zurich
2019-2023
Washington University in St. Louis
2017-2020
University of Iceland
2020
Noguchi Memorial Institute for Medical Research
2020
University of Ghana
2020
Solid state nuclear magnetic resonance (NMR) enables atomic-resolution characterization of the molecular structure and dynamics within complex heterogeneous samples, but it is typically insensitive. Dynamic polarization (DNP) increases NMR signal intensity by orders magnitude can be performed in combination with magic angle spinning (MAS) for sensitive, high-resolution spectroscopy. Here we report MAS DNP experiments, first time, intact human cells >40-fold enhancement a sample temperature...
Dynamic nuclear polarization (DNP) can enhance NMR sensitivity by orders of magnitude transferring spin from electron paramagnetic resonance (EPR) to NMR. However, DNP polarizing agents have deleterious effects on signals. Electron decoupling mitigate these relaxation effects. We demonstrate experiments in conjunction with and magic-angle-spinning spectroscopy. Following a diffusion period, the microwave irradiation frequency is quickly tuned on-resonance electrons agent. The performance...
Spherical rotors can be used for magic angle spinning nuclear magnetic resonance.
Fermentation products, together with food components, determine the sense, nutrition, and safety of fermented foods. Traditional methods fermentation product identification are time-consuming cumbersome, which cannot meet increasing need for extensive bioactive metabolites produced during fermentation. Hence, we propose a data-driven integrated platform (FFExplorer, http://www.rxnfinder.org/ffexplorer/) based on machine learning data 2,192,862 microbial sequence-encoded enzymes computational...
We demonstrate the construction of 7 Tesla and 12 all high-temperature-superconducting (HTS) magnets, small enough to fit on your wrist. The size magnet reduces cost fabrication, decreases fringe field permit facile siting stored energy high magnets. These HTS-based magnets are being developed for gyrotron microwave sources use in high-field nuclear magnetic resonance applications. employ a no-insulation winding technique cooled 4.2 Kelvin liquid helium cryostat. is single pancake coil, made...
We demonstrate for the first time in-cell dynamic nuclear polarization (DNP) in conjunction with flow cytometry sorting to address cellular heterogeneity of samples. Utilizing a green fluorescent protein (GFP) reporter HIV reactivation, we correlate increased 15N resonance intensity cytokine-driven reactivation human cell line model latency. As few as 10% GFP+ cells could be detected by DNP magnetic (NMR). The inclusion cytometric prior analysis DNP-NMR further boosted signal detection...
Spherical rotors are a new paradigm in magic angle spinning (MAS) solid-state nuclear magnetic resonance (NMR).The simple geometry makes smaller diameter spheres and their utilization within narrow-bore NMR probes feasible.Here we report 68 kHz frequency of 2 mm using helium gas outside the magnet demonstrate use KMnO 4 to adjust at 59.3 for MAS NMR.We observe third-order sidebands 55 Mn spectrum clearly showing kHz, with KBr nearly no first-order similar frequency.The stability was ±0.5%...
Abstract We present a compact 23 T no-insulation (NI) magnet that was wound with 60 m of 10 mm wide high temperature superconducting (HTS) tape. The consists only one pocket-sized double pancake (DP) coil an inner diameter ∼6 mm, height 20 and outer 41.6 mm. Another NI similar size but larger 8 reached slightly lower magnetic field 21 T. also smaller which HTS tape still achieved 16 During the experiments in liquid helium, each charged to current between 690 A 850 A, corresponding density...
A significant effort has recently been initiated at ETHZ for the development of high frequency, power, frequency-agile gyrotrons DNP-NMR spectroscopy. In this context an in house cavity interaction code is under development. paper, some details on new and preliminary results will be presented.
Precise alignment of the sample's spinning axis is vital for many magic angle solid state NMR experiments. Spherical rotors are a new paradigm NMR, having been demonstrated to spin stably with little risk rotor crash, but like cylindrical rotors, they have previously only utilized mechanical adjustment method set rotation angle. Here we show that by using second gas aperture within stator, spherical rotor's may be precisely pitched about without or motion stator. The 2H MAS sideband...
The no-insulation (NI) high-temperature superconductor (HTS) winding technique enables the fabrication of highly compact magnets with self-quench protection. NI pancake coils are implemented to develop a 28-T HTS magnet for operation 792-GHz gyrotron, which is microwave source dynamic nuclear polarization magnetic resonance. To this end, three all-GdBCO double (DP) were fabricated using different diameters and procedures. objective was explore mechanical coil protection mechanisms at high...
Abstract. The use of spherical rotors for magic angle spinning offers a number advantages, including improved sample exchange, efficient microwave coupling dynamic nuclear polarization magnetic resonance (NMR) experiments, and, most significantly, high frequency and stable with minimal risk rotor crash. Here we demonstrate the simple retrofitting commercial NMR probe MAS spheres solid-state NMR. We analyze series turbine groove geometries to investigate importance surface performance. Of...
<p>The no-insulation (NI) high temperature superconductor (HTS) winding technique enables the fabrication of highly compact magnets with self-quench protection. NI pancake coils are implemented to develop a 28 T HTS magnet for operation 792 GHz gyrotron, which is microwave source dynamic nuclear polarization magnetic resonance. To this end, three all-GdBCO double (DP) were fabricated using different diameters and procedures. The objective was explore mechanical coil protection...
Continuous wave (CW) dynamic nuclear polarization (DNP) is used with magic angle spinning (MAS) to enhance the typically poor sensitivity of magnetic resonance (NMR) by orders magnitude. In a recent publication we show that further enhancement obtained using frequency-agile gyrotron chirp incident microwave frequency through electron during DNP transfer. Here characterize effect chirped MAS investigating sweep time, width, center-frequency, and Rabi chirps. We advantages trityl nitroxide...
<p>The no-insulation (NI) high temperature superconductor (HTS) winding technique enables the fabrication of highly compact magnets with self-quench protection. NI pancake coils are implemented to develop a 28 T HTS magnet for operation 792 GHz gyrotron, which is microwave source dynamic nuclear polarization magnetic resonance. To this end, three all-GdBCO double (DP) were fabricated using different diameters and procedures. The objective was explore mechanical coil protection...
Abstract. The use of spherical rotors for magic angle spinning offers a number advantages including improved sample exchange, efficient microwave coupling dynamic nuclear polarization magnetic resonance (NMR) experiments and, most significantly, high frequency and stable with minimal risk rotor crash. Here we demonstrate the sim- ple retrofitting commercial NMR probe MAS spheres solid-state NMR. We analyze series turbine groove geometries to investigate importance surface on performance. Of...