The Schizosaccharomyces pombe spo20-KC104 mutation was originally isolated in a screen for sporulation-deficient mutants, and the mutant exhibits temperature-sensitive growth. Herein, we report that S. pombe, spo20 + is essential fission yeast cell viability constitutively expressed throughout life cycle. We also demonstrate thespo20 gene product structurally homologous to Saccharomyces cerevisiae Sec14, major phosphatidylinositol transfer protein of budding yeast. This structural homology...

Sporulation in the fission yeast Schizosaccharomyces pombe is a unique biological process that plasma membrane of daughter cells assembled de novo within mother cell cytoplasm. A double unit called forespore (FSM) constructed dynamically during meiosis. To obtain dynamic view FSM formation, we visualized living by using green fluorescent protein fused with Psy1, an FSM-resident protein, together nucleus or microtubules. The assembly initiates prophase II, and four FSMs expand synchronous...

Rheb GTPase and the Tsc1-Tsc2 protein complex, which serves as a GTPase-activating for Rheb, play critical roles in regulation of cell growth response to extracellular conditions. In Schizosaccharomyces pombe, regulate cycle progression, onset meiosis, uptake amino acids. cells lacking Tsc2 (Δtsc2), acid transporter Aat1, is normally expressed on plasma membrane under starvation conditions, confined Golgi. Here, we show that loss either pub1+, encoding an E3 ubiquitin ligase, or any1+,...

The spindle pole body (SPB) of Schizosaccharomyces pombe is required for assembly the forespore membrane (FSM) during meiosis. Before de novo biogenesis FSM, meiotic SPB forms outer plaques, an event referred to as modification. A constitutive component, Spo15, plays indispensable role in modification and sporulation. Here, we analyzed two sporulation-specific genes, spo13 + spo2 , which are not progression nuclear divisions, but essential Spo13 a 16-kDa coiled-coil protein, Spo2 15-kDa...

Abstract Rheb, a Ras-like small GTPase conserved from human to yeast, controls Tor kinase and plays central role in the regulation of cell growth depending on extracellular conditions. Rhb1 (a fission yeast homolog Rheb) regulates amino acid uptake as well response nitrogen starvation. In this study, we generated two mutants, rhb1-DA4 rhb1-DA8, characterized them genetically. The V17A mutation within G1 box defined for GTPases was responsible Q52R I76F switch II domain rhb1-DA8. events—the...

In Schizosaccharomyces pombe, neither intracellular sorting nor ubiquitination of amino acid permeases is well understood. the present study, we show that permease Aat1p in S. pombe depends on presence a nitrogen source growth medium. Under nitrogen-sufficient conditions, appeared to be stably localized at Golgi apparatus. contrast, under nitrogen-insufficient was sorted plasma membrane. Over time, membrane-localized internalized and lumen vacuole, where it degraded. Sorting vacuolar...

CENP-A determines the identity of centromere. Because position and size centromere its number per chromosome must be maintained, distribution is strictly regulated. In this study, we have aimed to understand mechanisms regulate (Cnp1SP) in fission yeast. A mutant ufd1+ gene (ufd1-73) encoding a cofactor Cdc48 ATPase sensitive Cnp1 expressed at high level allows mislocalization Cnp1. The centromeric chromatin increased ufd1-73 even when normal level. preexisting cdc48+ (cdc48-353) phenocopies...

Mutations in the human Tsc1 and Tsc2 genes predispose to tuberous sclerosis complex (TSC), a disorder characterized by wide spread of benign tumors. proteins form serve as GTPase-activating protein (GAP) for Rheb, GTPase regulating downstream kinase, mTOR. The genome Schizosaccharomyces pombe contains tsc1(+) tsc2(+), homologs Tsc2, respectively. In this study we analyzed gene expression profile on genomewide scale found that deletion either or tsc2(+) affects induction upon nitrogen...

The fission yeast spo20 + gene encodes a glycerophospholipid‐transfer protein. mutants are unable to assemble the forespore membrane properly. Here we studied structural integrity of spindle pole body (SPB) in spo20‐H6 during meiosis. Meiotic cells expressing GFP‐tagged SPB marker protein, Spo15‐GFP, showed an excess number SPBs, some which were not localized poles and termed ‘pseudo‐SPBs’. Formation spindles for meiosis I was significantly delayed cells, although morphology segregation...

Cells cease to proliferate above their growth-permissible temperatures, a ubiquitous phenomenon generally attributed heat damage cellular macromolecules. We here report that, in the presence of rapamycin, potent inhibitor Target Rapamycin Complex 1 (TORC1), fission yeast

Abstract Mei4 is a key sporulation-specific transcription factor in fission yeast. Ectopic expression of vegetative cells caused formation nucleated membranous compartments, which shared common features with normal forespore membranes, thereby perturbing nuclear division. These results suggest why development-specific factors must be strictly controlled.

The centromere is a chromosomal locus where microtubule attachment site, termed kinetochore, assembled in mitosis. In most eukaryotes, with the exception of holocentric species, each chromosome contains single distinct centromere. A an additional undergoes successive rounds anaphase bridge formation and breakage, or triggers cell cycle arrest imposed by DNA damage replication checkpoints. We report here study Schizosaccharomyces pombe to characterize mutant (cnp3-1) gene encoding homolog...

The human TSC2 gene, mutations in which predispose individuals to the disease tuberous sclerosis complex (TSC), encodes a GTPase-activating protein for GTPase RHEB. Loss of results constitutive activation RHEB and its target mammalian rapamycin (mTOR). We have previously reported that fission yeast (Schizosaccharomyces pombe) Tf2 retrotransposons (hereafter Tf2s) are abnormally induced upon nitrogen starvation cells lacking tsc2+ gene (Δtsc2), homolog with dominant-active mutation...

Myocardial calcification secondary to acute myocarditis is a rare but possibly life-threatening complication. We report 43-year-old woman with minimal change nephrotic syndrome who developed sepsis caused by Escherichia coli. simultaneously detected the complication of in patient. Although echocardiography showed hypokinesis apical segment when was diagnosed, no sign myocardial observed. After two weeks, CT same area. still observed 12 months later, patient's cardiac function had improved.

A 71-year-old Japanese man with progressive kidney failure was referred to our hospital. Laboratory tests showed elevated IgG4 levels. Contrast-enhanced computed tomography (CT) revealed soft tissue surrounding the left and right atrophic kidney. histopathological examination inflammation fibrosis rich IgG4-positive cells in thickened capsule, but not parenchyma. Poor enhancement on contrast-enhanced CT wrinkling of glomerular capillaries pathological tissues were also observed. These...

Abstract Cells cease to proliferate above their growth-permissible temperatures, a ubiquitous phenomenon generally attributed protein denaturing and heat damage other cellular macromolecules. We here report that, in the presence of macrolide compound rapamycin, fission yeast Schizosaccharomyces pombe can at high temperatures that normally arrest its growth. Rapamycin is potent inhibitor kinase complex TOR Complex 1 (TORC1), consistently, mutations TORC1 subunit RAPTOR/Mip1 substrate Sck1...

Abstract CENP-A, a variant of histone H3, determines the identity centromere, chromosome locus at which microtubule attachment site termed kinetochore is assembled. Because position and size centromere its number per must be maintained for faithful segregation chromosomes, distribution CENP-A strictly regulated. In this study, we have aimed to understand mechanisms regulate by genetic approach in fission yeast Schizosaccharomyces pombe . A mutant ufd1 + gene ( ufd1-73 ) encoding cofactor...

Cells cease to proliferate above their growth-permissible temperatures, a ubiquitous phenomenon generally attributed protein denaturing and heat damage other cellular macromolecules. We here report that, in the presence of macrolide compound rapamycin, fission yeast Schizosaccharomyces pombe can at high temperatures that normally arrest its growth. Rapamycin is potent inhibitor kinase complex TOR Complex 1 (TORC1), consistently, mutations TORC1 subunit RAPTOR/Mip1 substrate Sck1...