The Munc13/UNC-13 family protein Ync13 is essential for septum integrity and cytokinesis in fission yeast. To further explore the mechanism of functions, spontaneous suppressors ync13 mutants, which can suppress colony-formation defects lysis phenotype mutant cells, are isolated characterized. One suppressor bst1- s27, shows cytokinetic contractile ring constriction, septation, daughter-cell separation, similar to bst1Δ mutant. Bst1, a predicted GPI inositol deacylase, was an uncharacterized...

MADS6, JAGGED (JAG), and DROOPING LEAF (DL) are key regulators of floral organ patterns in rice (Oryza sativa); however, how they work together specifying organs remains to be determined. Here, we extensively analyzed the coordination mechanism. Genetic interactions showed that all double/triple mutant combinations mads6-5 with jag and/or dl-sup7 generated an inflorescence from spikelet center lemma-like (LLOs) at periphery, indicating these genes synergistically promote specification,...

Cytokinesis is crucial for integrating genome inheritance and cell functions. In multicellular organisms, Rho-guanine nucleotide exchange factors (GEFs) Rho GTPases are key regulators of division-plane specification contractile-ring formation during cytokinesis, but how they regulate early steps cytokinesis in fission yeast remains largely unknown. Here we show that putative Rho-GEF Gef2 Polo kinase Plo1 coordinate to control the medial cortical localization function anillin-related protein...

The evening complex (EC) plays a critical role in photoperiod flowering Arabidopsis. Nevertheless, the underlying functions of individual components and coordinate regulation mechanism EC genes rice remain to be elucidated. Here, we characterized LUX ARRHYTHMO (LUX) perception coordinating vegetative growth rice. Non-functional alleles OsLUX extremely extended phase, leading photoperiod-insensitive late great increase grain yield. displayed an obvious diurnal rhythm expression with peak at...

Rho GTPases regulate the actin cytoskeleton in all eukaryotes. Fission yeast Cdc42 is involved cable assembly and formin For3 regulation. We isolated cdc42-879 as a thermosensitive strain with localization defects. In multicopy suppressor screening, we identified pob1(+) of thermosensitivity. Pob1 overexpression also partially restores cables mutant strain. interacts this GTPase regulates and/or stability. The C-terminal pleckstrin homology (PH) domain required for binding. binds to through...

Cytokinesis is the last step of cell-division cycle, which requires precise spatial and temporal regulation to ensure genetic stability. Rho guanine nucleotide exchange factors (Rho GEFs) GTPases are among key regulators cytokinesis. We previously found that putative Rho-GEF Gef2 coordinates with Polo kinase Plo1 control medial cortical localization anillin-like protein Mid1 in fission yeast. Here we show an adaptor protein, Nod1, colocalizes contractile ring its precursor nodes. Like gef2,...

Abstract A major question about cytokinesis concerns the role of septin proteins, which localize to division site in all animal and fungal cells but are essential for only some cell types. For example, Schizosaccharomyces pombe, four septins site, deletion genes produces a modest delay separation. To ask if S. pombe function redundantly cytokinesis, we conducted synthetic-lethal screen septin-deficient strain identified seven mutations. One mutation affects Cdc4, myosin light chain that is...

Background and Purpose H 2 S induces vasodilatation by opening K ATP channels but it may also affect other ion channels. The aim of this study was to investigate the effect on intestinal motility in rats its underlying mechanism. Experimental Approach tension muscle strips, afferent firing mesenteric nerves, length duodenal smooth cells whole‐cell membrane potential dorsal root ganglion ( DRG ) neurons were monitored. S‐producing enzymes located immunofluorescence staining. Key results NaHS...

Both farnesyl diphosphate synthase (FPS) and geranylgeranyl (GGPS) are key enzymes in the synthesis of various isoprenoid-containing compounds proteins. Here, we describe two novel Schizosaccharomyces pombe genes, fps1(+) spo9(+), whose products similar to FPS primary structure, but functions differ from one another. Fps1 is essential for vegetative growth, whereas, a spo9 null mutant exhibits temperature-sensitive growth. Expression fps1(+), not suppresses lethality Saccharomyces cerevisiae...

The spindle pole body (SPB) of Schizosaccharomyces pombe is required for assembly the forespore membrane (FSM) during meiosis. Before de novo biogenesis FSM, meiotic SPB forms outer plaques, an event referred to as modification. A constitutive component, Spo15, plays indispensable role in modification and sporulation. Here, we analyzed two sporulation-specific genes, spo13 + spo2 , which are not progression nuclear divisions, but essential Spo13 a 16-kDa coiled-coil protein, Spo2 15-kDa...

OsMADS16, a class B floral organ identity gene, plays pivotal role in stamen formation rice. To date, little is known about the interacting partners of OsMADS16 except for several MADS-box proteins. In this study, we constructed high-quality cDNA library young panicles (< 5 cm length) and performed yeast two-hybrid (Y2H) screening using as bait. Eleven candidate proteins with were identified by Y2H validated BiFC Co-IP assays. Subcellular localization results further confirmed possibility...

RETINOBLASTOMA-RELATED (RBR) is an essential gene in plants, but its molecular function outside of role cell cycle entry remains poorly understood. We characterized the functions OsRBR1 and OsRBR2 plant growth development rice using both forward- reverse-genetics methods. The two genes were coexpressed performed redundant roles vegetative organs exhibited separate flowers. was highly expressed floral meristem regulated expression homeotic to ensure organ formation. Mutation caused loss...

Monascus -fermented quinoa alleviates the hyperlipidemia in mice induced by a high-fat diet regulating metabolic pathways such as phenylalanine, tyrosine, and tryptophan metabolism.

Pom2 is predicted to be a dual-specificity tyrosine-phosphorylation regulated kinase (DYRK) related Pom1 in Schizosaccharomyces pombe. DYRKs share domain capable of catalyzing autophosphorylation on tyrosine and exogenous phosphorylation serine/threonine residues. Here we show that functionally different from the well-characterized Pom1, although they 55% identity complements Pom1. localizes mitochondria throughout cell cycle contractile ring during late stages cytokinesis. Overexpression...

Crop reproductive development is vulnerable to heat stress, and the genetic modulation of thermotolerance during phase, especially early stage, remains poorly understood. We isolated a Poaceae-specific FAR-RED ELONGATED HYPOCOTYLS3 (FHY3)/FAR-RED IMPAIRED RESPONSE1 (FAR1)family transcription factor, Thermo-sensitive Spikelet Defects 1 (TSD1), derived from transposase in rice (Oryza sativa) TSD1 was highly expressed spikelets, induced by heat, specifically enhanced spikelet morphogenesis....

Sugar transporter protein family is very common among different organisms in nature and plays a key role the transportation, absorption, synthesis of secondary metabolites. To investigate functional characteristics sugar proteins Monascus pilosus, cDNA sequence gene MpST collected from M. pilosus CICC 5045 was cloned this research. Furthermore, bioinformatics methods molecular docking techniques were applied to analyze protein. The obtained results revealed that coding region 1677 bp length,...

The methanol content and volatile aroma components were determined, sensory evaluation analysis conducted. Results showed that the contents of all six sweet potato wine samples met limits national standard, a total 258 substances detected. Among them, alcohols phenols have highest in sample BBC-1 brewed family workshops, esters BBY-2 distillery, acids ketones BBC-4 aldehydes BBY-1 distillery. results BBC-2 has strongest most elegant aroma, perfect typicality, unique taste. comprehensive are...

Monacolin K (MK), produced by Monascus fermentation, has significant hypolipidemic effects. In this study, under solid-state fermentation (SSF) quinoa was used as substrate to investigate the effect of MK production species quinoa. The results single factor experiment showed that amount loaded, addition water substrate, and temperature change time have a on Monascus-fermented It found neither M. pilosus-fermented nor ruber-fermented citrinin. Consequently, these three factors were selected...

Abstract Stromal cells in the tumor microenviroment play an important role development and progression of nasopharyngeal carcinoma (NPC). Aim this study is to identify stromal cell related genes which may promote distant metastasis NPC. Materials methods: Differently expressed were accessed by comparing microarray. Then analyzed using statistics tools. Five selected validated 112 specimens. Results: We have identified 3360 up-regulated (P &amp;lt; .05)and 2327 down-regulated(P .05) versus...