A5083415520- Nuclear Structure and Function
- RNA Research and Splicing
- Plant Reproductive Biology
- Neurobiology and Insect Physiology Research
- Plant Molecular Biology Research
- RNA modifications and cancer
- Biotin and Related Studies
- Cellular transport and secretion
- Animal Behavior and Reproduction
- Insect Resistance and Genetics
- Plant and animal studies
- Insect Utilization and Effects
- Genomics and Chromatin Dynamics
- Photosynthetic Processes and Mechanisms
- Invertebrate Immune Response Mechanisms
- Insect and Pesticide Research
- Insect-Plant Interactions and Control
- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
- Medicinal Plants and Neuroprotection
- Cell death mechanisms and regulation
- Genetic Mapping and Diversity in Plants and Animals
- Moringa oleifera research and applications
Hainan University
2020-2025
University of California, Berkeley
2020-2025
Peking University
2023-2024
Sichuan University
2024
West China Hospital of Sichuan University
2024
Tsinghua University
2018-2023
Huanggang Central Hospital
2017-2022
China Agricultural University
2015-2022
State Key Laboratory of Animal Nutrition
2022
Innovative Genomics Institute
2021-2022
Insect phenoloxidase, presented as an inactive precursor prophenoloxidase (PPO) in hemolymph, catalyzes melanin formation, which is involved wound healing, pathogen killing, reversible oxygen collection during insect respiration, and cuticle eggshell formation. Mosquitoes possess 9 to 16 PPO members across different genera, a number that more than found other dipteran insects. However, the reasons for redundancy of these PPOs whether they have distinct biochemical properties physiological...
Abstract The inner nuclear membrane (INM) selectively accumulates proteins that are essential for functions; however, overaccumulation of INM results in a range rare genetic disorders. So far, little is known about how defective, mislocalized, or abnormally accumulated actively removed from the INM, especially plants and animals. Here, via analysis proximity-labeling proteomic profile INM-associated Arabidopsis , we identify critical components an protein degradation pathway. We show this...
The regulated nucleocytoplasmic exchange of macromolecules is essential for the eukaryotic cell. However, nuclear transport pathways defined by different receptors (NTRs), including importins and exportins, their significance in activating distinct stress responses are poorly understood plants. Here, we exploited a CRISPR/Cas9-based genetic screen to search modifiers CONSTITUTIVE EXPRESSION OF PATHOGENESIS-RELATED GENE 5 (cpr5), an Arabidopsis thaliana nucleoporin mutant that activates...
Abstract In flowering plants, male gametes are delivered to female for double fertilization through pollen tubes. Therefore, tube growth is crucial fertilization. Despite its importance sexual reproduction, genetic mechanisms of remain poorly understood. this study, we characterized the receptor‐like cytoplasmic protein kinase (RLCK) gene, MARIS ( MRI ) that plays critical roles in growth. preferentially expressed grains, tubes and roots. Mutation by a Ds insertion led burst after...
The nuclear basket (NB) is an essential structure of the pore complex (NPC) and serves as a dynamic multifunctional platform that participates in various critical processes, including cargo transport, molecular docking, gene expression regulation. However, underlying mechanisms are not completely understood, particularly plants. Here, we identified guanylate-binding protein (GBP)-like GTPase (GBPL3) novel NPC component Arabidopsis . Using fluorescence immunoelectron microscopy, found GBPL3...
Meiotic recombination is initiated from the formation of DNA double-strand breaks (DSBs). In Arabidopsis, several proteins, such as AtPRD1, AtPRD2, AtPRD3, AtDFO and topoisomerase (Topo) VI-like complex, have been identified playing important roles in DSB formation. Topo complex Arabidopsis may consist subunit A (Topo VIA: AtSPO11-1 AtSPO11-2) B VIB: MTOPVIB). Little known about their Here, we report on characterization MTOPVIB gene using mutant alleles mtopVIB-2 mtopVIB-3, which were...
Abstract Coronary heart disease is a kind of which causes great injury to people world‐widely. Although gene expression analyses had been performed previously, our best knowledge, systemic co‐expression analysis for this still lacking date. Microarray data coronary was downloaded from NCBI with the accession number GSE20681. Co‐expression modules were constructed by WGCNA. Besides, connectivity degree eigengenes analyzed. Furthermore, GO and KEGG enrichment on these in modules. A total 11...
Eukaryotic cells organize their genome within the nucleus with a double-layered membrane structure termed nuclear envelope (NE) as physical barrier. The NE not only shields but also spatially separates transcription from translation. Proteins of including nucleoskeleton proteins, inner and pore complexes have been implicated in interacting underlying chromatin regulators to establish higher-order architecture. Here, I summarize recent advances knowledge proteins that are involved...
Abstract Little is known about the blood‐feeding physiology of arbovirus vector Aedes aegypti although this type mosquito to transmit infectious diseases dengue, Zika, yellow fever, and chikungunya. Blood feeding in female A. essential for egg maturation transmission disease agents between human subjects. Here, we identify sulfakinin receptor gene SKR from genome show that expressed at different developmental stages varied anatomical localizations adult (at three days after eclosion), with...
Arylalkylamine N-acetyltransferase (aaNAT) catalyzes the acetylation of dopamine, 5-hydroxy-tryptamine, tryptamine, octopamine, norepinephrine and other arylalkylamines to form respective N-acetyl-arylalkylamines. Depending on products formed, aaNATs are involved in a variety physiological functions. In yellow fever mosquito, Aedes aegypti, number aaNAT-like proteins have been reported. However, primary function each individual aaNAT is yet be identified. this study we investigated Ae....
Abstract Detection of chemical cues via chemosensory receptor proteins are essential for most animals, and underlies critical behaviors, including location discrimination food resources, identification sexual partners avoidance predators. The current knowledge how detected is based primarily on data acquired from studies insects, while our understanding the molecular basis chemoreception in acari, mites particular, remains limited. poultry red mite (PRM), Dermanyssus gallinae , one important...
Arylalkylamine N-acetyltransferase (aaNAT) in Aedes aegypti is primarily involved cuticle pigmentation and formation. The reported arylalkylamine substrates are all primary amines. In this study, we report a novel substrate, secondary amine, of Ae. aaNAT1. recombinant aaNAT1 protein exhibited high activity to epinephrine, which has not been for any aaNATs previously. Structure-activity relationship study demonstrated that an epinephrine-binding site, molecular docking dynamic simulation...
In flowering plants, the male and female gametogenesis is a crucial step of sexual reproduction. Although many genes have been identified as being involved in process, genetic mechanisms underlying remains poorly understood. We reported here characterization gene, ABORTED GAMETOPHYTE 1 (AOG1) that newly essential for Arabidopsis thaliana. AOG1 expressed predominantly reproductive tissues including developing pollen grains ovules. The protein shares no significant amino acid sequence...
Summary Organ size is an important agronomic trait. Small peptides function in various stages of plant growth, but their regulatory mechanisms organ growth remain poorly understood. Here, we characterize a novel small peptide, AtZSP1, which positively regulates Arabidopsis. Loss‐of‐function mutant atzsp1‐1 exhibited organs, whereas AtZSP1 overexpression plants (p 35S:AtZSP1 #1) produced larger organs. Differentially expressed genes the shoots and p #1 were enriched cytokinin pathway. Further...
Previously, we isolated several genes that regulate the expression of type III secretion system (T3SS) in Ralstonia solanacearum OE1-1strain. Here, focused on rsp0316, which encodes a putative 2-nitropropane dioxygenase (hereafter designated NpdA). The deletion npdA substantially reduced T3SS and virulence compared to wild-type strain OE1-1, complemented could completely restore its OE1-1 strain. NpdA was highly conserved R. species NpdA-dependent not specific strain, but particularly...
Arylalkylamine N-acetyltransferase (aaNAT), considered a potential new insecticide target, catalyzes the acetylation of arylalkylamine substrates such as serotonin and dopamine and, hence, mediates diverse functions in insects. However, origin insect aaNATs (iaaNATs) evolutionary process that generates multiple mosquitoes remain largely unknown. Here, we have analyzed genomes 33 species to explore expand our understanding molecular evolution this gene family detail. We show aaNAT orthologs...