A5004179364- Transplantation: Methods and Outcomes
- Cardiac and Coronary Surgery Techniques
- Organ Transplantation Techniques and Outcomes
- Renal Transplantation Outcomes and Treatments
- Angiogenesis and VEGF in Cancer
- Cardiac Valve Diseases and Treatments
- Ubiquitin and proteasome pathways
- Animal Virus Infections Studies
- Cardiac electrophysiology and arrhythmias
- Cardiac, Anesthesia and Surgical Outcomes
- Surgical Simulation and Training
- Mechanical Circulatory Support Devices
- Heart rate and cardiovascular health
- Heart Failure Treatment and Management
- Airway Management and Intubation Techniques
- Coronary Interventions and Diagnostics
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Nerve Injury and Rehabilitation
- Cardiac Structural Anomalies and Repair
- Cardiac Imaging and Diagnostics
- Venous Thromboembolism Diagnosis and Management
- Reconstructive Surgery and Microvascular Techniques
- Cardiac Ischemia and Reperfusion
University of Leeds
2018-2025
Manchester University NHS Foundation Trust
2012-2020
University of Manchester
2012-2020
Manchester Academic Health Science Centre
2017-2020
Centre for Inflammation Research
2014-2018
Wythenshawe Hospital
2009-2017
Edge Hill University
2017
Passenger leukocyte transfer from the donor lung to recipient is intrinsically involved in acute rejection. Direct presentation of alloantigen expressed on leukocytes recognized by T cells, promoting cellular We utilized ex vivo perfusion (EVLP) study passenger migration lungs into and evaluate effects depletion prior transplantation. For this purpose, female pigs received male left either following 3 h EVLP or retrieved using standard protocols. Recipients were monitored for 24 sequential...
Ex vivo normothermic perfusion offers an alternative method of organ preservation, allowing donor kidneys to be reanimated and evaluated prior transplantation. Beyond it can used characterize the immunological contribution kidney in isolation. Furthermore, has potential as immunomodulatory strategy manipulate transplantation.Explanted porcine underwent 6 hours perfusion. Sequential perfusate samples were collected leukocytes characterized via flow cytometry. An inflammatory profile was...
The use of an open vein harvesting (OVH) technique for saphenous (SVH) is associated with wound complications and delayed patient mobilization. This has led to the development minimally invasive (MIVH) techniques, such as standard bridging endoscopic SVH (EVH). randomized trial was established assess immediate clinical outcome satisfaction in our centre.A total 150 consecutive patients were prospectively into three groups. Group 1 consisted 50 who underwent OVH, 2 a (SBT) 3 EVH. Each group...
Current consensus statements maintain that endoscopic vein harvesting (EVH) should be standard care in coronary artery bypass graft surgery, but quality and clinical outcomes have been questioned. The VICO trial (Vein Integrity Clinical Outcomes) was designed to assess the impact of different methods on vessel damage whether this contributes after grafting.In single-center, randomized trial, patients undergoing grafting with an internal mammary 1 4 grafts were recruited. All veins harvested...
Surgical knots on the suture line provide an anchoring function, but also represent a potential source of infection and irritation donor leg after coronary artery bypass surgery. Knotless barbed sutures were designed to prevent knot-related complications. This study compared wound complication rates between patients receiving traditional monofilament those knotless for closure leg. One hundred forty-two randomized into two groups. Group 1 (n = 70) received 2 72) sutures. All wounds assessed...
The introduction of endoscopic saphenous vein harvesting (ESVH) has been reported to decrease saphenectomy-associated wound pain and infection, compared with the traditional open conventional (OCSVH) technique. Despite all these benefits, rate adoption among surgeons variable. Criticism this technique centres on risk injury at time harvest its potential detrimental effect structural viability long-term patency. aim our study is investigate endothelial preservation grafts harvested by various...
Introduction: Many donor organs contain significant leukocyte reservoirs which upon transplantation activate recipient leukocytes to initiate acute rejection. We aimed assess whether ex vivo heart perfusion promotes immunodepletion and alters the inflammatory status of organ prior transplantation. Methods: Isolated porcine hearts underwent hypothermic, cardioplegic for 8 hours. Leukocyte populations were quantified in left ventricle samples by flow cytometry. Cell-free DNA, cytokines...
It remains possible that the benefit from beta-blockers (BBs) in chronic heart failure (CHF) may not entirely be derived a class-specific effect. Several experimental reports have alluded to capability of immunomodulation by individual BBs. Given increasingly recognized importance immune system pathogenesis CHF, we studied effects BBs on circulating these patients. Blood samples CHF outpatients were prospectively analyzed using flow cytometry and gating software. Results against...
OBJECTIVESEndoscopic vein harvesting is one of the most popular minimally invasive vein-harvesting techniques for coronary artery bypass graft surgery. It associated with improved cosmetic outcome and fewer wound-related problems compared conventional open technique. However, its efficacy regard to conduit damage long-term patency has recently been questioned. Learning curve-associated trauma a major impact on quality incidence failure post-surgery. In an attempt address this problem, we...
Abstract Increased resting heart rate is an independent modifiable risk factor for the development of cardiovascular disease. Numerous studies have demonstrated improved clinical outcomes with reduction in patients coronary artery disease and failure, but its role transplanted hearts not yet established. Sinus tachycardia more common transplant recipients due to graft denervation. Although a large number recognized increased as predictor native atherosclerosis overall cardiac mortality,...
Investigators have proposed that cardiovascular magnetic resonance (CMR) should restrictions similar to those of ionizing imaging techniques. We aimed investigate the acute effect 1.5 T CMR on leucocyte DNA integrity, cell counts, and function in vitro, a large cohort patients vivo.In vitro study: peripheral blood mononuclear cells (PBMCs) were isolated from healthy volunteers, histone H2AX phosphorylation (γ-H2AX) expression, functional parameters quantified using flow cytometry under...
Abstract The SARS-CoV-2 coronavirus is responsible for the COVID-19 outbreak, which overwhelmed millions of people worldwide; hence, there an urgency to identify appropriate antiviral drugs. This study focuses on screening compounds that inhibit RNA-dependent RNA-polymerase (RdRp) essential RNA synthesis required replication positive-strand viruses. Computational against RdRp using Food and Drug Administration (FDA)-approved drugs identified ten prominent with binding energies more than −...
Vascular endothelial growth factor receptor 2 (VEGFR2) regulates function and angiogenesis. VEGFR2 undergoes ubiquitination which programs this for trafficking proteolysis but the ubiquitin-modifying enzymes involved are ill-defined. Herein, we used a reverse genetics screen of human E2 family ubiquitin-conjugating to identify gene products regulate proteolysis. We find that depletion either UBE2D1 or UBE2D2 in cells cause rise steady-state levels. This plasma membrane levels impact on...
In multicellular organisms, a variety of lipid-protein particles control the systemic flow triacylglycerides, cholesterol and fatty acids between cells in different tissues. The chemical modification by oxidation these can trigger pathological responses, mediated group membrane proteins termed scavenger receptors. lectin-like oxidised low-density lipoprotein (LOX-1) receptor binds to oxidized low density (oxLDL) mediates both signaling trafficking outcomes. Here, we identified 5 synthetic...
Objective The aim of the study was to assess whether use carbon dioxide insufflation has any impact on integrity long saphenous vein comparing 2 types endoscopic harvesting and traditional open harvesting. Methods A total 301 patients were prospectively randomized into 3 groups. Group 1 control arm (n = 101), group closed tunnel (carbon dioxide) 100) (open harvesting) 100). Each assessed determine systemic level partial arterial dioxide, end-tidal pH. Three blood samples obtained at...
Background. Donor kidneys contain a large reservoir of passenger leucocytes that contribute to acute rejection via direct alloantigen presentation and pro-inflammatory cytokine secretion. However, the early contribution these cells following revascularization has not previously been described. We performed secondary, high-volume preservation flush cold storage characterize inflammatory donor kidney upon reperfusion. Methods. Porcine were retrieved using protocol analogous current UK clinical...