A5102013180- Evolution and Paleontology Studies
- Paleontology and Evolutionary Biology
- Bacteriophages and microbial interactions
- CRISPR and Genetic Engineering
- Viral gastroenteritis research and epidemiology
- Genetic Associations and Epidemiology
- Amphibian and Reptile Biology
- Botanical Research and Applications
- Biosensors and Analytical Detection
- Catalytic Processes in Materials Science
- Pleistocene-Era Hominins and Archaeology
- Ichthyology and Marine Biology
- Catalysis and Oxidation Reactions
- Salmonella and Campylobacter epidemiology
- Literary Theory and Cultural Hermeneutics
- Cell Image Analysis Techniques
- Listeria monocytogenes in Food Safety
- Genomics and Rare Diseases
- Ecology and biodiversity studies
- Synthesis and Reactivity of Heterocycles
- Language, Metaphor, and Cognition
- Air Quality and Health Impacts
- Media, Communication, and Education
- Plant Gene Expression Analysis
- Cassava research and cyanide
Genomics England
2024
King's College London
2024
The University of Texas at Austin
2010-2023
LabCorp (United States)
2020-2023
University of Leicester
2012
Petrified Forest National Park
2010
Bristol-Myers Squibb (Germany)
2000
Yellow and red-violet betalain plant pigments are restricted to several families in the order Caryophyllales, where betacyanins play analogous biological roles anthocyanins. The initial step biosynthesis is hydroxylation of tyrosine form L-DOPA. Using gene expression experiments beets, yeast, Arabidopsis, along with HPLC/MS analysis, present study shows that two novel cytochrome P450 (CYP450) enzymes, CYP76AD6 CYP76AD5, previously described CYP76AD1 can perform this step. Co-expressing these...
Host range is a major determinant in the industrial utility of bacteriophage. A model host permits broad recognition across serovars target bacterium while avoiding cross-reactivity with commensal microbiota. Searching for naturally occurring bacteriophage ideal ranges challenging, time-consuming, and restrictive. To address this, SPTD1.NL, previously published luciferase reporter Salmonella, was used to investigate manipulation through receptor-binding protein engineering. Similar related...
Summary The key enzymatic step in betalain biosynthesis involves conversion of l ‐3,4‐dihydroxyphenylalanine ( ‐ DOPA) to betalamic acid. One class enzymes capable this is 3,4‐dihydroxyphenylalanine 4,5‐dioxygenase DODA ). In betalain‐producing species, multiple paralogs gene are maintained. This study demonstrates which function the pathway and determines residue changes required evolve a betalain‐nonfunctional into betalain‐functional . Functionalities two pairs s were tested by expression...
Abstract Salmonella is a major causative agent of foodborne illness and rapid identification this pathogen essential to prevent disease. Currently most assays require high bacterial burdens or prolonged enrichment achieve acceptable performance. A reduction in testing time without loss sensitivity critical allow food processors safely decrease product holding time. To meet need, method was developed detect using luciferase reporter bacteriophages. Bacteriophages were engineered express...
Physical examination and study of fossil vertebrates often requires partial or complete removal delicate elements from a protective substrate. Although necessary for research, such extensive exp...
Bacteriophages have been investigated for clinical utility, both as diagnostic tools and therapeutic interventions. In order to be applied successfully, a detailed understanding of the influence human matrix on interaction between bacteriophage host bacterium is required. this study, cocktail luciferase reporters was assessed functionality in containing serum spiked with Staphylococcus aureus. The inhibition signal loss sensitivity evident minimal amounts serum. This phenotype independent...
Engineered luciferase reporter bacteriophages provide specific, sensitive, rapid and low-cost detection of target bacteria address growing diagnostic needs in multiple industries. Detection methicillin-resistant Staphylococcus aureus (MRSA) nasal colonization antibiotic susceptibility play a critical supportive role preventing hospital-acquired infections facilitating stewardship. We describe the development evaluation novel phage-based MRSA screen for swab specimens. The utilizes two phages...
Field crews from The University of Texas at Austin first identified pterosaur remains the Upper Cretaceous Javelina Formation Big Bend National Park in 1971 and continued excavation these animals for decades. announcement giant Quetzalcoatlus northropi 1975 by graduate student Douglas Lawson drew worldwide attention, fossil preparators William Amaral Robert Rainey discovered several key localities a region informally called Pterodactyl Ridge that have been thoroughly collected documented....
A practical synthesis of the anti-methicillin resistant Staphylococcus aureus cephem (6R-trans)-E-7-[[[[2,5-dichloro-4-[3-[(carboxymethyl)amino]-3-oxo-1-propenyl]phenyl]-thio]-acetyl]amino]-4-[[(2-carboxy-8-oxo-5-thia-1-azabicyclo-[4.2.0]oct-2-en-3-yl)methyl]thio]-2,6-dimethyl-1-[3-(4-methylmorpholino-4-yl)propyl]-1-pyridinium, hydroxide, inner salt (BMS-247243) was developed. process developed for interchange iodide counterion in 3a to chloride 3b that essential an efficient C-3 side chain...
ABSTRACT Vertebrate fossils have been converted from natural history objects into research specimens through the act of preparation for over 200 years. All basic techniques applied to in 21st Century were already use palaeontological laboratories by first decade 1900s. It behoves any worker field be intimately familiar with processes treatment specimens, as these procedures almost always permanently alter material available interpretation. Historic treatments also complicate attempts...
Abstract The lack of bacteriophages capable infecting the Listeria species, grayi , is academically intriguing and presents an obstacle to development bacteriophage-based technologies for . We describe isolation engineering a novel L. bacteriophage, LPJP1, isolated from farm silage. With genome over 200,000 base pairs, LPJP1 first only reported jumbo bacteriophage genus. Similar other Gram-positive phages, appeared contain modified which complicated initial attempts obtain genomic sequence...
Abstract Whole genome sequencing identifies millions of genetic variants per individual. When applied to rare disease diagnosis, potentially pathogenic are prioritised for clinical interpretation, a process that may be influenced by an individual’s ancestry. We analysed protein-altering in 29,425 participants with from the UK 100,000 Genomes Project. observed disparities number across ancestry groups, up 3-fold increase African compared European ancestries. Variants non-European ancestries...
Abstract Purpose Most research on genetic screening and precision oncology is based participants of European ancestry, making it vital to evaluate the performance these approaches in diverse populations. We analysed data from 100,000 Genomes Project (100kGP) assess ancestry-related differences cancer variant prioritisation. Patients Methods To representativeness 14,775 with 100kGP, we compared recruitment ratios for self-reported ethnicities those England. For ancestry groups detection rates...
Engineered bacteriophages (phages) can be effective diagnostic reporters for detecting a variety of bacterial pathogens. Although promising biotechnology, the large-scale use these may result in unintentional release genetically modified viruses. In order to limit potential environmental impact, ability phages propagate outside laboratory was targeted. The phage SEA1 has been previously engineered facilitate food safety as an accurate and sensitive reporter Salmonella contamination. this...
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The purpose of this application, under Article 81.1 the Code, is to conserve widely used binomen Quetzalcoatlus northropi in its accustomed usage by making generic and specific names available attributing authorship Lawson (1975b).
A reduced mesoporous 5% Pd-YSZ precatalyst was observed to be very active for the Heck reaction providing up 770,000 TON. Three-phase tests have experimentally confirmed that a leached homogeneous species is responsible activity. Residual Pd concentration in solution minimal (<0.10 ppm) and therefore this material has potential applications because it can easily removed by filtration. This paper describes synthesis, structural characterization, catalytic activity of material. The prepared...
Fossils have been collected from the field in plaster and burlap jackets for more than a century.These support protect contained fossils until they can be exposed under controlled conditions laboratory.The challenging nature of work complications caused by limited time, adverse weather, or supply shortages often produce suboptimal that are less protective difficult to remove.Often, growth vegetation through rock poorly consolidated sediments compromises stability jacket contents.In such...
AbstractWe report the presence of high levels endotoxin in and low colony forming unit (CFU/g) cannabis flower samples. In some jurisdictions, samples over 100,000 CFU/g total aerobic count (TAC), are prioritized for decontamination or remediation using ozone, E-beam, X-ray gamma ray exposure. While these methods well known reducing viable units, they less effective at removing bacterial DNA endotoxin. Less is about residual bioactive that might exist on such decontaminated products. To...