Abstract Diabetic ketoacidosis (DKA) is a life‐threatening acute complication of diabetes characterized by the accumulation ketone bodies in blood. Breath acetone, ketone, directly correlates with blood ketones. Therefore, monitoring breath acetone can significantly enhance safety and efficacy care. In this work, design fabrication an InP/Pt/chitosan nanowire array‐based chemiresistive sensor reported. By incorporation chitosan as surface‐functional layer Pt Schottky contact for efficient...

Polysaccharides have key biological functions and can be harnessed for therapeutic roles, such as the anticoagulant heparin. Their complexity-e.g., >100 monosaccharides with variety in linkage branching structure-significantly complicates analysis compared to other biopolymers DNA proteins. More, improved, tools been called for, here we demonstrate that solid-state silicon nitride nanopore sensors tuned sensing conditions used reliably detect native polysaccharides enzymatic digestion...

Proteins are arguably one of the most important class biomarkers for health diagnostic purposes. Label-free solid-state nanopore sensing is a versatile technique and analyzing biomolecules such as proteins at single-molecule level. While molecular-level information on size, shape, charge can be assessed by nanopores, identification with comparable sizes remains challenge. Here, combined machine learning to address this The translocations four similarly sized using amplifiers bandwidths (BWs)...

With growing interest in solid-state nanopore sensing─a single-molecule technique capable of profiling a host analyte classes─establishing facile and scalable approaches for fabricating molecular-size pores is becoming increasingly important. The introduction fabrication by controlled breakdown (CBD) has transformed the economics accessibility fabrication. Here, we introduce design an Arduino-based, portable USB-powered CBD device, with estimated cost <150 USD, which ≈10–100× cheaper than...

In this study, we investigated the voltage and pH responsiveness of human serum transferrin (hSTf) protein using silicon nitride (SixNy) nanopores. The Fe(III)-rich holo form hSTf was dominant when > pI, while Fe(III)-free apo < pI. translocations were purely in an electrophoretic sense, thus depended on its pI solution pH. With increasing voltage, driven unfolding became prominent which indicated by trends associated with change conductance, due to translocation, excluded electrolyte...

Solid-state nanopores (SSNs) are single-molecule resolution sensors with a growing footprint in real-time bio-polymer profiling-most prominently, but far from exclusively, DNA sequencing. SSNs accessibility has increased the advent of controlled dielectric breakdown (CDB), severe fundamental challenges remain: drifts open-pore current and (irreversible) analyte sticking. These behaviors impede basic research device development for commercial applications can be dramatically exacerbated by...

Solid-state nanopore technology delivers single-molecule resolution information, and the quality of deliverables hinges on capability analysis platform to extract maximum possible events fit them appropriately. In this work, we present an with four baseline fitting methods adaptive a wide range traces (including those step or abrupt changes where pre-existing platforms fail) maximize extractable (2× improvement in some cases) multilevel event capability. The methods, increasing order...

Abstract Chemiresistive sensing is one of the most promising technologies for portable and miniaturized chemical sensing, with applications ranging from air quality monitoring to explosive detection medical diagnostics. Recently, there have been growing efforts in developing microchip based sensors operating at room temperature high sensitivity, selectivity, spatial temporal resolution, long‐term stability, cost‐effectiveness. Here, engineering highly performing gas consisting chemiresistive...

Abstract Stability, long lifetime, resilience against clogging, low noise, and cost are five critical cornerstones of solid‐state nanopore technology. Here, a fabrication protocol is described wherein &gt;1 million events obtained from single with both DNA protein at the highest available lowpass filter (LPF, 100 kHz) Axopatch 200B–the event count mentioned in literature. Moreover, total ≈8.1 reported this work encompassing two analyte classes. With kHz LPF, temporally attenuated population...

Nanopores are a prominent enabling tool for single-molecule applications such as DNA sequencing, protein profiling, and glycomics, the construction of ionic circuit elements. Silicon nitride (SiNx) is leading scaffold these <100 nm-diameter nanofluidic ion-conducting channels, but frequently challenging surface chemistry remains an obstacle to their use. We functionalized more than 100 SiNx nanopores with different terminations-acidic (Si-R-OH, Si-R-CO2H), basic (Si-R-NH2), nonionizable...

Solid-state nanopore based electro-deformation coupled with deep learning to distinguish AAV particles on their cargo content.

Controlled dielectric breakdown (CDB) of silicon nitride thin films immersed in electrolyte solution has been used to fabricate single nanofluidic channels with ∼10 nm and smaller diameters, nanopores, useful single-molecule sensing ionic circuit construction. A hand-held Tesla-coil lighter was form conductors through a thick membrane. Modifications the conventional approach were required by low-overhead Tesla-coil-assisted method (TCAM): increased resistance including water place discrete...

Figure shows hSTf protein translocating through a solid-state nanopore under an applied electric field and the resulting current traces. The transport mechanism is determined by interplay of electrophoretic electroosmotic force.

Thin membranes are highly sought-after for nanopore-based single-molecule sensing, and fabrication of such becomes challenging in the ≲10 nm thickness regime where a plethora useful molecule information can be acquired by nanopore sensing. In this work, we present scalable controllable method to fabricate silicon nitride (SixNy) with effective down ∼1.5 using standard processing chemical etching hydrofluoric acid (HF). Nanopores were fabricated controlled breakdown estimated pore diameters...

A method to directly electrolessly plate silicon-rich silicon nitride with thin gold films was developed and characterized. Films thicknesses <100 nm were grown at 3 10 °C between 0.5 h, mean grain sizes ∼20 30 nm. The is compatible plating free-standing ultrathin membranes, we successfully plated the interior walls of micropore arrays in 200 thick membranes. thus amenable coating planar, curved, line-of-sight-obscured surfaces.

Abstract This paper describes a method to gauge the stiffness of nanosized liposomes – nanoscale vesicle using custom‐made recapture platform coupled solid‐state nanopore sensor. The electrically profiles given liposome multiple times through automated reversal voltage polarity immediately following translocation instance re‐translocate same analyte provides better statistical insight at molecular level by analyzing particle compared conventional platforms. capture frequency depends on...

We describe a method for simply characterizing the size and shape of nanopore during solution-based fabrication surface modification, using only low-overhead approaches native to conventional measurements. Solution-based methods are democratizing science by supplanting traditional use charged-particle microscopes fabrication, but profiling has customarily depended on microscopic examination. Our approach exploits dependence conductance in solution size, shape, chemistry order characterize...

Solid-state nanopores are nanoscale channels through otherwise impermeable membranes. Single molecules or particles can be passed electrolyte-filled by, e.g. electrophoresis, and then detected the resulting physical displacement of ions within nanopore. Nanopore size, shape, surface chemistry must carefully controlled, on extremely challenging <10 nm-length scales. We previously developed a framework to characterize from time-dependent changes in their conductance as they being formed...

Abstract The development of nanoscale optical sensors is desirable for a broad range applications, including wearable medical‐diagnostics, biochemical detection, and environmental monitoring. Optical detection platforms based on resonant nanostructures are the golden standard miniaturized footprint high sensitivity. These function by measuring shift in resonance wavelength upon binding analytes to their surface. However, such measurements sensitive intensity fluctuations illuminating source...

Abstract The success of a nanopore experiment relies not only on the quality experimental design but also performance analysis program utilized to decipher ionic perturbations necessary for understanding fundamental molecular intricacies. An event extraction framework is developed that leverages parallel computing, efficient memory management, and vectorization, yielding significant enhancement. newly abf‐ultra‐simple function extracts key parameters from header critical operation...