Bisphenol A (BPA), known as an environmental endocrine disruptor, is widely used a plasticizer. This study aims to investigate whether exposure in utero BPA alters the architecture, proliferative index, and genomic signature of rat mammary gland during critical stages development. Pregnant rats were gavaged with 25 microg BPA/kg body weight (BW; low-dose group) or 250 BW (high-dose from day 10 post-conception delivery. Female litters euthanized at 21, 35, 50, 100 days, glands collected....

Environmental estrogens are exogenous estrogen-mimicking compounds that can interfere with endogenous endocrine systems. Several of these disruptors have been shown to alter normal development and influence tumorigenesis in experimental models. N-butyl benzyl phthalate (BBP), a widely used plasticizer, is well-known disruptor. The aim this study was elucidate the effect prenatal exposure BBP on morphology, proliferative index, genomic signature rat mammary gland at different ages. In utero...

We have postulated that the lifetime protective effect of an early pregnancy against breast cancer is due to complete differentiation mammary gland characterized by a specific genomic signature imprinted physiological process pregnancy. For demonstrating this hypothesis we compared profile epithelium and stroma normal tissues from reduction mammoplasties performed in postmenopausal parous nulliparous women. The were separately dissected using laser capture microdissection (LCM) RNA each...

Disruption of epigenetic patterns is a major change occurring in all types cancers. Such alterations are characterized by global DNA hypomethylation, gene-promoter hypermethylation and aberrant histone modifications, may be modified environment. Nutritional factors, especially dietary lipids, have role the etiology breast cancer. Thus, we aimed to analyze influence different high fat diets on methylation modifications rat dimethylbenz(a)anthracene (DMBA)-induced cancer model. Female...

The aim of this study was to investigate the effects two phenolic compounds found in extra virgin olive oil, hydroxytyrosol (HT) and luteolin (LUT), on metabolism breast cancer (BC) cells different molecular subtypes. An untargeted metabolomics approach used characterize metabolic responses both triple-negative MDA-MB-231 hormone-responsive MCF-7 treatment with these phenols. Notably, while some were common across cell types, others dependent type, highlighting importance cellular phenotype....

Effects of a high virgin olive oil diet on the promotion stage dimethylbenz(alpha)anthracene-induced mammary carcinogenesis in rats were investigated comparison with those corn diet. Animals randomly placed into 4 groups: control, fed normolipidic control (3% oil); M, (20%); O, plus 17% and MO, after 33 days Whereas clearly stimulated carcinogenesis, reducing latency time increasing tumor incidence, multiplicity volume, led to lower incidence than former group, similar that volume even...

Abstract Background Phthalate esters like n-butyl benzyl phthalate (BBP) are widely used plasticizers. BBP has shown endocrine-disrupting properties, thus having a potential effect on hormone-sensitive tissues. The aim of this study is to determine the neonatal/prepubertal exposure (post-natal days 2–20) maturation parameters and morphology, proliferative index genomic signature rat mammary gland at different ages development (21, 35, 50 100 days). Results Here we show that increased uterine...

Renal fibrosis is characterized by an increased number of fibroblasts and excessive deposition extracellular matrix. Apoptotic cell death a physiological mechanism to limit numbers, insufficient rate may contribute fibroblast accumulation. However, little known about the regulation renal survival. The authors have studied interaction cytokines Fas receptor in apoptosis observed that murine express ligand. Tumor necrosis factor alpha (TNFalpha) agonistic anti-Fas antibodies induce time-...

In previous studies, we demonstrated that high corn oil diets promote the development of 7,12-dimethylbenz(alpha)anthracene (DMBA)-induced mammary tumors. this study, have investigated whether modulation gene expression is one mechanisms by which high-fat diet exerts such effects. Female Sprague-Dawley rats were induced with DMBA and fed normolipidic (3% oil) or (20% diet. Screening genes differentially expressed in adenocarcinomas from group compared to control was performed cDNA...

Extra-virgin olive oil (EVOO) has been hypothesized to have chemopreventive effects on breast cancer, unlike high corn (HCO) diets that stimulate it. We investigated mechanisms of these differential modulatory actions experimental mammary cancer. In 7,12-dimethylbenz(a)anthracene adenocarcinomas rats fed a EVOO, HCO and control ( n = 20 for each group), we analyzed the expression activity ErbB receptors, p21Ras its extracellular signal-regulated kinase (ERK) 1/2, Akt RalA/B effectors by...

Based on the importance of early-life events in breast cancer risk, we have investigated effects high-fat diets maturation, mammary gland development, and its susceptibility to transformation. Female Sprague-Dawley rats were fed a lowfat (LF), high corn oil (HCO), or extra-virgin olive (HOO) diet from weaning gavaged with 7,12-dimethylbenz[a]anthracene. Body weight mass increased HCO group compared LF group. The vaginal opening was advanced both groups, especially This also had body around...

High extra-virgin olive oil (EVOO) and corn diets differentially modulate experimental mammary carcinogenesis. We have investigated their influence on the initiation stage through modulation of expression xenobiotic-metabolizing enzymes (XMEs) in liver gland. Female Sprague–Dawley rats were fed a low-fat (LF), high (HCO), or EVOO (HOO) diet from weaning gavaged with 7,12-dimethylbenz(a)anthracene (DMBA). The HCO increased mRNA levels phase I CYP1A1, CYP1A2 and, to lesser extent, CYP1B1,...

Breast cancer is the most common malignancy in women worldwide, and dietary lipids are important environmental factors influencing its etiology. We have investigated effects, mechanisms associated, of high-fat diets on 7,12-dimethylbenz(a)anthracene-induced rat mammary tumors. Animals were fed a low-fat, high-corn-oil (HCO) or high-extra-virgin-olive-oil (HOO) diet from weaning after induction. The HCO had clear stimulating effect carcinogenesis, especially when intervention started...