Background and Objectives: Chronic rhinosinusitis with nasal polyps (CRSwNP) presently remains a difficult disease to manage. Antimicrobial defense proteins are important factors that could help characterize the role of microorganisms in CRSwNP pathogenesis, as concept microbial dysbiosis CRS is still being considered. Our aim investigate complex appearance, relative distribution interlinks human β defensin 2 (HBD-2), 3 (HBD-3), 4 (HBD-4), cathelicidin LL 37 (LL 37) chronic (CRSwNP)-affected...

Background and Objectives. The aim of this study was to compare the distribution proliferation markers (Ki-67, NF-κβ), tissue-remodeling factors (MMP-2, MMP-9, TIMP-2, TIMP-4), vascular endothelial growth factor (VEGF), interleukins (IL-1 IL-10), human beta defensins (HβD-2 HβD-4) Sonic hedgehog gene protein in cholesteatoma control skin. Methods. Nineteen patient tissues seven skin materials from cadavers were included stained immunohistochemically. Results. Statistically discernible...

Background and Objectives: Chronic rhinosinusitis (CRS) is a condition that affects as much 10.9% of the population and, along with presence nasal polyps, associated significant morbidity decreased quality life. Studies on molecular pathways have been activated in polyp tissue are mainly based cytokine concentration detection. Therefore, our aim to investigate complex appearance, relative distribution interlinks IL-1, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12 Ki 67 chronic polyps (CRSwNP)...

Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a chronic inflammation of the mucosa nose and paranasal sinuses presence polyps, affecting between 2.7% 4.4% population. Cytokine analysis has become important in research on inflammatory mechanisms CRSwNP. Therefore, our aim to investigate complex appearance, relative distribution, interlinks IL-1, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12, Ki-67 Methods: Samples were obtained from 19 patients previously diagnosed CRSwNP recurrence...

The aim of this study was to describe the appearance and distribution tissue remodeling markers (MMP-2, MMP-9, TIMP-2, TIMP-4), Sonic hedgehog gene protein (Shh), pro- anti-inflammatory cytokines (IL-1, IL-10), transcription factor (NF-κβ), proliferation marker (Ki-67), angiogenetic (VEGF), defensins (HβD-2, HβD-4) pediatric cholesteatoma. Sixteen cholesteatoma samples were obtained from children, eleven skin controls cadavers. Tissues stained for MMP-2, TIMP-4, Shh, IL-1, IL-10, NF-κβ,...

Vocal nodules (or Singer's nodules) are benign vocal cord structures which commonly encountered by clinicians. Though phonetic trauma/abuse is thought to be the main cause of development nodules, exact etiopathogenesis remains unknown. Hence, we compared immunohistochemical markers for proliferation (Ki-67), apoptosis (TUNEL), growth (EGFR), ischemia (VEGF), inflammation (IL-1α and 10), immunoreactive innervation (PGP 9.5), in nodule tissue samples obtained from 10 females (17-56 years)...

The upper respiratory tract harbors diverse communities of commensal, symbiotic, and pathogenic organisms, originating from both the oral nasopharyngeal microbiota. Among primary sites microbial colonization in airways are adenoids. Alterations adenoid microbiota have been implicated development various conditions, including secretory otitis media.This study aims to employ 16S rRNA genetic sequencing identify most common bacteria present on surface adenoids children with media effusion...

OBJECTIVE The post nasal drip is a very common symptom of sinusitis, allergic rhinosinusopathy, the gastroesophageal reflux disease, but there are some patients, who have drip, sensation foreign body in nasopharynx and non-specific irritant cough with no other symptoms or signs sinus inflammations allergy. In posterior rhinoscopy mucus discharge seen. Ethiology pathogenesis this syndrome evolution still unclear. aim study was to identify neuropeptide appearance distribution nasopharyngeal...

Background and Objectives: the upper respiratory tract harbors highest bacterial density in whole system. Adenoids, which are located nasopharynx, a major site of colonies airways. Our goal was to use culture-independent molecular techniques identify breadth diversity adenoid vegetations children suffering from chronic rhinosinusitis obstructive sleep apnea. Materials methods: total, 21 samples were investigated using amplification sequencing V3-V4 hypervariable region 16S rRNA gene....

Background: An acquired cholesteatoma is a benign but locally aggressive lesion in the middle ear. It characterized by chronic inflammation and destruction of surrounding bone. Therefore, aim this study was to compare defensins HβD-2 HβD-4; pro- anti-inflammatory cytokines IL-1α IL-10; proliferation marker Ki-67; transcription factor NF-κβ; angiogenetic VEGF; Sonic hedgehog gene protein SHH; remodeling factors MMP-2, MMP-9, TIMP-2, TIMP-4 adult pediatric tissue, these groups with control...

The adenoids, primary sites of microbial colonization in the upper airways, can influence development various conditions, including otitis media with effusion (OME). Alterations adenoid microbiota have been implicated pathogenesis such conditions.

The main goal of our study was to describe the transcription factor (NF-κβ), angiogenetic (VEGF), and remodeling markers (MMP-9 TIMP-4) cholesteatoma tissue compared control skin tissue. There are still uncertainties how transcription, factors affect course.Eight specimens were retrieved from children, seven - adults, controls cadavers. Obtained material immunohistochemically stained for NF-κβ, MMP-9, TIMP-4, VEGF. Non-parametric statistic methods used.A statistically significant higher...

The objective of the present study was to elucidate distribution interleukins and cytokines in mucous membranes nose nasopharynx patients presenting with post-nasal drip syndrome. included 20 this pathological condition volunteers who comprised control group. samples were examined use immunohistochemical methods identify protein gene product 9.5, interleukin-6, interleukin-10, tumour necrosis factor-alpha, nuclear factor-kB, beta-defensin. It shown that syndrome is characterized by enhanced...

The nasal cavity lined by mucosa, is a significant part of respiratory system human. However, there are no studies aimed to detect molecular phenotype healthy and normal functioning obtained after rhinoseptoplasty procedure, understand its physiology growth inflammation processes. Thus, our aim identify human mucosa cytokines, neuropeptide-containing innervation cell proliferation markers form control group for further tissue investigation polyposis as the next step research. study included...

Smoking, laryngopharyngeal reflux, and vocal fold abuse can promote the development of Reinke's oedema, leading to dysfunction injury. The aim work was investigate appearance distribution proliferation marker Ki-67 (Ki-67), interleukin 10 (IL-10), 1 alpha (IL-1α), protein gene peptide 9.5 (PGP 9.5) in oedema-affected larynx tissue.A routine histological immunohistochemical oedema control group patient analysis conducted. We used biotin-streptavidin biochemical method detect Ki-67, IL-10,...

∗Pauls Stradins Clinical University Hospital, Rīga Stradiņš †Pauls Riga, Latvia Address correspondence and reprint requests to Alise Adoviča, M.S., University, Dzirciema iela 16, Riga 1007, Latvia; E-mail: [email protected] The authors disclose no conflicts of interest.

Abstract Background: Reinke’s oedema (benign diffuse swelling of the vocal folds) induced various morphological changes occurs in superficial lamina propria also known as space. Smoking, laryngopharyngeal reflux and fold abuse can promote development this condition well lead to dysfunction injury. Patients with complain problems. 3,47/1000 is estimation general population prevalence oedema. Investigation distribution appearance Ki-67, IL-1α, IL-10 PGP 9.5 oedema-affected larynx tissue...

Cleft lip and palate are the most common congenital malformations which require early primary cleft surgery in all infants. The surgical treatment of deformities results formation scar tissue. However, scarred tissue has sparsely been immunohistochemically evaluated to date. Herein, we report differences cellular expression various proteins nearby healthy a 35-year-old patient who underwent correction during infancy. showed basal cell proliferation, prominent cysts, fibrotic connective An...

Objective: Tonsillectomy, cryptolysis of the tonsils and removal various formations in pharynx are most common elective operations otorhinolaryngology. Pain early postoperative period is still complication these procedures, which can adversely affect patient's daily life. Considering potential analgesic anti-inflammatory effect essential oil preparations, as well their ability to be easily applied locally affected mucosal surface, it worth considering use reduce pain dysphagia upper...

In the pedagogy of classical vocal singing, it can be difficult to determine human voice fach, especially for aspiring vocalists. Hence, an objective metric-based system determination is needed. present study, we investigated anthropological and aerodynamic parameters 60 professional singers with a professionally confirmed singing range.